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Acta Parasitologica

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Volume 60, Issue 2 (Jun 2015)

Issues

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

Silvana Carnevale
  • Corresponding author
  • Instituto Nacional de Enfermedades Infecciosas – ANLIS “Dr. Carlos G. Malbran”, Avenida Velez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
  • Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Avenida Rivadavia 1917, (CP 1033), Ciudad de Buenos Aires, Argentina;
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María Laura Pantano
  • Instituto Nacional de Enfermedades Infecciosas – ANLIS “Dr. Carlos G. Malbran”, Avenida Velez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Laura Kamenetzky
  • Instituto de Microbiologia y Parasitologia Medica (IMPaM), UBA-CONICET, Paraguay 2155 piso 13, (CP 1121), Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jorge Bruno Malandrini
  • Facultad de Ciencias de la Salud, Universidad Nacional de Catamarca, Maestro Quiroga 1ra. Cuadra, (CP 4700) San Fernando del Valle de Catamarca, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Claudia Cecilia Soria
  • Facultad de Ciencias de la Salud, Universidad Nacional de Catamarca, Maestro Quiroga 1ra. Cuadra, (CP 4700) San Fernando del Valle de Catamarca, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jorge Néstor Velásquez
  • Hospital Municipal de Infecciosas “Dr. Francisco Javier Muniz”, Uspallata 2272, (CP 1282) Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0030

Abstract

Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.

Keywords: Fasciola hepatica; PCR; faeces

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About the article

Received: 2014-05-28

Revised: 2014-10-26

Accepted: 2014-11-28

Published Online: 2015-03-25

Published in Print: 2015-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0030.

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