Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

4 Issues per year


IMPACT FACTOR 2016: 1.160
5-year IMPACT FACTOR: 1.185

CiteScore 2016: 1.24

SCImago Journal Rank (SJR) 2016: 0.532
Source Normalized Impact per Paper (SNIP) 2016: 0.721

Online
ISSN
1896-1851
See all formats and pricing
More options …
Volume 60, Issue 2 (Jun 2015)

Issues

Frequencies of dhfr/dhps multiple mutations and Plasmodium falciparum submicroscopic gametocyte carriage in Gabonese pregnant women following IPTp-SP implementation

Marielle K. Bouyou-Akotet
  • Corresponding author
  • Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante, Libreville, Gabon
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marie-Louise Tshibola
  • Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante, Libreville, Gabon
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Denise P. Mawili-Mboumba
  • Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante, Libreville, Gabon
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Julie Nzong
  • Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante, Libreville, Gabon
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Noemi Bahamontes-Rosa / Gladys Tsoumbou-Bakana
  • Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante, Libreville, Gabon
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maryvonne Kombila
Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0031

Abstract

This study analyzed the relationship between intermittent preventive treatment with sulfadoxine-pyrimethamine (SP) (IPTp-SP), the rate of multiple resistant parasites and of submicroscopic gametocyte carriage among pregnant women at the beginning of IPTp implementation in Gabon (2005) and six years after (2011). The detection of pfdhfr and pfdhps gene mutations was performed by PCR-RFLP in Plasmodium (P.) falciparum positive samples collected from pregnant women in 2005 and 2011. Gametocytes carriage was detected by Pfs25mRNA amplification using QT-NASBA. Data were analyzed according to the time of collection (study period) and IPTp-SP doses. The proportion of isolates with at least a triple Pfdhfr mutation (n = 39/42, 92.9% versus 100%, n = 78/78)) and of those isolates with the S108N/C59R/N51I/S436A/A437G multiple mutation (17.9% versus 75.6%) significantly increased between 2005 and 2011 (p<0.01). Mutations I164L and A581G were not found, while higher proportions of 436 and 437 mutations were detected in 2011.A trend toward a higher frequency of isolates with five mutations was observed in women who received two SP doses (p<0.01). Pfs25mRNA was found in 6.8 % (n = 3/44) and 34.6% (n = 27/78) of the samples collected in 2005 and 2011 respectively (p<0.01). In 2011, 74.0% (n = 20/27) of women with detected submicroscopic gametocytes carried parasites with the S108N/C59R/N51/S436A/A437G multiple mutation. All the ten delivering women who received three IPTp-SP doses had a submicroscopic Plasmodium falciparum infection, but none had detected gametocytes. Following IPTp-SP implementation, an increase in the frequency of multiple mutant parasites and of submicroscopic gametocyte carriage was observed among pregnant women living in Gabon.

Keywords: Malaria; Pregnancy; IPTp-SP; Resistance; Gametocytaemia

References

  • Aubouy A., Jafari S., Huart V., Migot-Nabias F., Mayombo J., Durand R., Bakary M., Le Bras J., Deloron P. 2003. DHFR and DHPS genotypes of Plasmodium falciparum isolates from Gabon correlate with in vitro activity of pyrimethamine and cycloguanil, but not with sulfadoxine-pyrimethamine treatment efficacy. Journal of Antimicrobial Chemotherapy, 53, 43-49CrossrefGoogle Scholar

  • Barnes K.I., Little F., Mabuza A., Mngomezulu N., Govere J., Durrheim D., Roper C., Watkins B., White N.J., 2008. Increased gametocytemia after treatment: an early parasitological indicator of emerging sulfadoxine-pyrimethamine resistance in falciparum malaria. Journal Infectious Diseases, 197, 1605-1613. DOI: 10.1086/587645Web of ScienceCrossrefGoogle Scholar

  • Bauer M., Polzin S., Patzelt D.2003. Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains? Forensic Science International, 138, 94-103CrossrefPubMedGoogle Scholar

  • Bertin G., Briand, V., Bonaventure D., Carrieu A., Massougbodji A., Cot M., Deloron P. 2011. Molecular markers of resistance to sulphadoxine-pyrimethamine during intermittent preventive treatment of pregnant women in Benin. Malaria Journal, 10, 196-203. DOI: 10.1186/1475-2875-10-196PubMedCrossrefGoogle Scholar

  • Bousema T., Drakeley, C. 2011. Epidemiology and infectivity of Plasmodium falciparum and Plasmodium vivax gametocytes in relation to malaria control and elimination. Clinical Microbiology Review, 24, 377-410. DOI: 10.1128/CMR.00051-10CrossrefWeb of ScienceGoogle Scholar

  • Bouyou-Akotet, M.K., Mawili-Mboumba D.P., Kendjo E., Mabika- Mamfoumbi M., Ngoungou E.B., Dzeing-Ella A., Pemba-Mihindou M., Ibinga E., Efame-Eya E., MCRU team., Planche T., Kremsner P.G., Kombila M. 2009. Evidence of decline of malaria in the general hospital of Libreville, Gabon from 2000 to 2008. Malaria Journal, 8, 300-308Web of ScienceGoogle Scholar

  • Bouyou-Akotet M.K., Mawili-Mboumba D.P., Kombila M. 2013. Antenatal care visit attendance, intermittent preventive treatment and bed net use during pregnancy in Gabon. BMC Pregnancy and Childbirth, 13, 52-57. DOI: 10.1186/1471-2393-13-52Web of ScienceCrossrefGoogle Scholar

  • Bouyou-Akotet M.K., Nzenze-Afene S., Ngoungou E.B., Kendjo E., Owono-Medang M., Lekana-Douki J.B., Obono-Obiang G., Mounanga M., Kombila M., 2010a. Burden of malaria during pregnancy at the time of IPTp/SP implementation in Gabon. American Journal of Tropical Medicine and Hygiene, 82, 202-209. DOI: 10.4269/ajtmh.2010.09-0267CrossrefGoogle Scholar

  • Bouyou-Akotet M.K., Mawili-Mboumba D.P., Tchantchou T.D., Kombila M., 2010b. High prevalence of sulfadoxine/pyrimethamineresistant alleles of Plasmodium falciparum isolates in pregnant women at the time of introduction of intermittent preventive treatment with sulfadoxine/pyrimethamine in Gabon. Journal of Antimicrobial Chemotherapy, 65, 438-41. DOI: 10.1093/ jac/dkp467Web of ScienceCrossrefGoogle Scholar

  • Duraisingh M.T., Curtis J., Warhurst, D.C. 1998. Plasmodium falciparum: detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion. Experimental Parasitology, 9, 1-8CrossrefGoogle Scholar

  • Eisele T.,P., Larsen D.A., Anglewicz P.A., Keating J., Yukich J., Bennett A., Hutchinson P., Steketee R.W. 2012.. Malaria prevention in pregnancy, birthweight, and neonatal mortality: a meta-analysis of 32 national cross-sectional datasets in Africa. Lancet Infectious Diseases, 12, 942-949. DOI: 10.1016/ S1473-3099(12)70222-0CrossrefWeb of ScienceGoogle Scholar

  • Gesase S., Gosling R.D., Hashim R., Ord R., Naidoo I., Madebe R., Mosha J.F., Joho A., Mandia V., Mrema H., Mapunda E., Savael Z., Lemnge M., Mosha F.W., Greenwood B., Roper C., Chandramohan D. 2009. High resistance of Plasmodium falciparum to sulphadoxine/pyrimethamine in northern Tanzania and the emergence of dhps resistance mutation at Codon 581. PLoS One, 4, e4569. DOI: 10.1371/journal.pone. 0004569CrossrefGoogle Scholar

  • Hallett R.L., Dunyo S., Ord R., Jawara M., Pinder M., Randall A., Alloueche A., Walraven G., Targett G.A., Alexander N., Sutherland C.J. 2006. Chloroquine/sulphadoxine-pyrimethamine for gambian children with malaria: transmission to mosquitoes of multidrug-resistant Plasmodium falciparum. PLoS Clinical Trials 2006, 1 (3):e15Google Scholar

  • Harrington W.E., Mutabingwa T.K., Muehlenbachs A., Sorensen B., Bolla M.C., Fried M., Duffy P.E. 2009. Competitive facilitation of drug-resistant Plasmodium falciparum malaria parasites in pregnant women who receive preventive treatment. Proceedings of the National Academy of Sciences of the United States of America, 106, 9027-32. DOI: 10.1073/ pnas.0901415106CrossrefGoogle Scholar

  • Iriemenam N.C., Shah M., Gatei W., van Eijk A.M., Ayisi J., Kariuki S., Vanden Eng J., Owino S.O., Lal A.A., Omosun Y.O., Otieno K., Desai M., ter Kuile F.O., Nahlen B., Moore J., Hamel M.J., Ouma P., Slutsker L., Shi Y.P. 2011. Temporal trends of sulphadoxine-pyrimethamine (SP) drug-resistance molecular markers in Plasmodium falciparum parasites from pregnant women in western Kenya. Malaria Journal, 11, 134-140. DOI: 10.1186/1475-2875-11-134CrossrefGoogle Scholar

  • Karl S., Gurarie D., Zimmerman P.A., King C.H., St Pierre T.G., Davis T.M. 2011. A sub-microscopic gametocyte reservoir can sustain malaria transmission. PLoS One, 6: e20805. DOI: 10.1371/journal.pone.0020805CrossrefGoogle Scholar

  • Kast K., Berens-Riha N., Zeynudin A., Abduselam N., Eshetu T., Loscher T., Wieser A., Shock J., Pritsch M. 2013. Evaluation of Plasmodium falciparum gametocyte detection in different patient material. Malaria Journal, 12, 438-447. DOI: 10.1186/1475-2875-12-438CrossrefPubMedGoogle Scholar

  • Lin J.T., Mbewe B., Taylor S.M., Luntamo M., Meshnick S.R., Ashorn P. 2013. Increased prevalence of dhfr and dhps mutants at delivery in Malawian pregnant women receiving intermittent preventive treatment for malaria. Tropical Medicine and International Health, 18, 175-8. DOI: 10.1111/tmi.12028CrossrefWeb of ScienceGoogle Scholar

  • Luntamo M., Rantala A.M., Meshnick S.R., Cheung Y.B., Kulmala T., Maleta K., Ashorn P. The effect of monthly sulfadoxinepyrimethamine, alone or with azithromycin, on PCR-diagnosed malaria at delivery: a randomized controlled trial. PLoS One. 2012, 7, e41123Google Scholar

  • Mawili-Mboumba D.P., Bouyou Akotet M.K., Kendjo E., Nzamba J., Medang M.O., Mbina J.R., Kombila M., MCORU team., 2013a. Increase in malaria prevalence and age of at risk population in different areas of Gabon. Malaria Journal, 12, 3-11. DOI: 10.1186/1475-2875-12-3Web of ScienceCrossrefGoogle Scholar

  • Mawili-Mboumba D.P., Bouyou Akotet M.K., Ngoungou E.B., Kombila M. 2010. Evaluation of rapid diagnostic tests for malaria case management in Gabon. Diagnostic Microbiology and Infectious Diseases, 66, 162-168. DOI: 10.1016/j.diagmicrobio. 2009.09.011CrossrefGoogle Scholar

  • Mawili-Mboumba D.P., Nikiema R.., Bouyou-Akotet M.K., Bahamontes- Rosa N., Traore A., Kombila M. 2013b. Sub-microscopic gametocyte carriage in febrile children living in different areas of Gabon. Malaria Journal, 2013;12:375-381. DOI: 10.1186/1475-2875-12-375Web of ScienceCrossrefGoogle Scholar

  • Menard D., Yapou F., Manirakiza A., Djalle D., Matsika-Claquin M.D., Talarmin A. 2006. Polymorphism in pfcrt, pfmdr1, dhfr genes and in vitro responses to antimalarials in Plasmodium falciparum isolates from Bangui, Central African Republic. American Journal of Tropical Medicine and Hygiene, 75, 381-387Google Scholar

  • Mendez F., Herrera S., Murrain B., Gutierrez A., Moreno L.A., Manzano M., Munoz A., Plowe C.V. 2007. Selection of antifolateresistant Plasmodium falciparum by sulfadoxine-pyrimethamine treatment and infectivity to Anopheles mosquitoes. American Journal of Tropical Medicine and Hygiene, 77, 438-343Google Scholar

  • Mombo-Ngoma G., Oyakhirome S., Ord R, Gabor J.J., Greutelaers K.C., Profanter K., Greutelaers B., Kurth F., Lell B., Kun J.F., Issifou S., Roper C., Kremsner P.G., Grobusch M.P. 2011. High prevalence of dhfr triple mutant and correlation with high rates of sulfadoxine-pyrimethamine treatment failures in vivo in Gabonese children. Malaria Journal, 10, 123-128. DOI: 10.1186/1475-2875-10-123Web of ScienceCrossrefGoogle Scholar

  • Ndiaye D., Dieye B., Ndiaye Y.D., Van Tyne D., Daniels R., Bei A.K., Mbaye A., Valim C., Lukens A., Mboup S., Ndir O., Wirth D.F., Volkman S. 2014. Polymorphism in dhfr/dhps genes, parasite density and ex vivo response to pyrimethamine in Plasmodium falciparum malaria parasites in Thies, Senegal. International Journal for Parasitology: Drugs and Drug Resistance, 3, 135-142. DOI: 10.1016/j.ijpddr.2013.07.001CrossrefWeb of ScienceGoogle Scholar

  • Nzila A.M., Mberu E.K., Sulo J., Dayo H., Winstanley P.A., Sibley C.H., Watkins W.M. 2000. Towards an understanding of the mechanism of pyrimethamine-sulfadoxine resistance in Plasmodium falciparum: genotyping of dihydrofolate reductase and dihydropteroate synthase of Kenyan parasites. Antimicrobial Agents and Chemotherapy, 44, 991-996PubMedCrossrefGoogle Scholar

  • Okell L.C., Bousema T., Griffin J.T., Ouedraogo A.L., Ghani A.C., Drakeley C.J. 2012. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nature Communication, 3, 1237-1246. DOI: 10.1038/ncomms2241CrossrefGoogle Scholar

  • Picot S., Olliaro P., de Monbrison F., Bienvenu A.L., Price R.N., Ringwald, P. 2009. A systematic review and meta-analysis of evidence for correlation between molecular markers of parasite resistance and treatment outcome in falciparum malaria.PubMedGoogle Scholar

  • Malaria Journal, 8, 89-98. DOI: 10.1186/1475-2875-8-89CrossrefPubMedGoogle Scholar

  • Schneider P., Bousema J.T., Gouagna L.C., Otieno S., van de Vegte- Bolmer M., Omar S.A., Sauerwein R.W. 2007. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. American Journal of Tropical Medicine and Hygiene, 76, 470-474Google Scholar

  • Schneider P., Bousema T., Omar, S., Gouagna L., Sawa, P., Schallig H., Sauerwein R., 2006. (Sub)microscopic Plasmodium falciparum gametocytaemia in Kenyan children after treatment with sulphadoxine-pyrimethamine monotherapy or in combination with artesunate. International Journal for Parasitology, 36, 403-408PubMedCrossrefGoogle Scholar

  • Taylor S.M., Antonia A.L., Chaluluka E., Mwapasa V., Feng G., Molyneux M.E., ter Kuile F.O., Meshnick S.R., Rogerson S.J. 2012. Antenatal receipt of sulfadoxine-pyrimethamine does not exacerbate pregnancy-associated malaria despite the expansion of drug-resistant Plasmodium falciparum: clinical outcomes from the QuEERPAM study. Clinical Infectious Diseases, 55, 42-50. DOI: 10.1093/cid/cis301CrossrefWeb of ScienceGoogle Scholar

  • World Health Organization, 2004. A Strategic Framework for Malaria Prevention and Control during Pregnancy in the Africa Region. WHO Regional Office for Africa. AFR/MAL/2004/01Google Scholar

  • World Health Organization, 2012. Intermittent Preventive Treatment of malaria in pregnancy using SulfadoxinePyrimethamine (IPTp-SP). Updated WHO Policy Recommendation. Global Malaria Programme Malaria Policy Advisory Committee Meeting 11-13 September 2012, WHO, HQ, Session 4 Google Scholar

About the article

Received: 2014-04-22

Revised: 2014-10-12

Accepted: 2014-12-05

Published Online: 2015-03-25

Published in Print: 2015-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0031.

Export Citation

© 2015 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
M.K. Bouyou-Akotet, D.P. Mawili-Mboumba, E. Kendjo, S. Moutandou Chiesa, M.L. Tshibola Mbuyi, G. Tsoumbou-Bakana, J. Zong, N. Ambounda, and M. Kombila
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2016, Volume 110, Number 6, Page 333
[2]
Jacques-Mari Ndong Ngomo, Denise Patricia Mawili-Mboumba, Noé Patrick M’Bondoukwe, Rosalie Nikiéma Ndong Ella, and Marielle Karine Bouyou Akotet
Malaria Research and Treatment, 2016, Volume 2016, Page 1

Comments (0)

Please log in or register to comment.
Log in