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Acta Parasitologica

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Volume 60, Issue 2


PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

Maria-Larisa Ivanescu
  • Department of Clinics, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, 3, M. Sadoveanu, 700490, Iasi, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dumitru Acatrinei
  • Department of Clinics, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, 3, M. Sadoveanu, 700490, Iasi, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ionuţ Pavel
  • Department of Clinics, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, 3, M. Sadoveanu, 700490, Iasi, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tatiana Sulesco
  • Institute of Zoology, Academy of Sciences of Moldova, 1 Academy Street, MD-2028, Chişinău, Moldova
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Liviu Miron
  • Corresponding author
  • Department of Clinics, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" University of Agricultural Sciences and Veterinary Medicine, 3, M. Sadoveanu, 700490, Iasi, Romania
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0040


The members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the Anopheles maculipennis complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of A. maculipennis among which: 58 A. atroparvus, 18 A. melanoon, 2 A. labranchiae, 52 A. maculipennis and 87 A. messeae. The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the A. maculipennis available in GenBank. The Species A. labranchiae is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the A. labranchiae sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.

Keywords: Anopheles maculipennis complex; Malaria; Europe


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About the article

Received: 2014-01-24

Revised: 2014-12-09

Accepted: 2015-01-09

Published Online: 2015-03-25

Published in Print: 2015-06-01

Citation Information: Acta Parasitologica, Volume 60, Issue 2, Pages 283–289, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0040.

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