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Acta Parasitologica

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Volume 60, Issue 2 (Jun 2015)

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Molecular evidence of Theileria equi infection in Hyalomma anatolicum ticks infested on sero-positive Indian horses

Jai Bhagwan
  • ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125 001, Haryana, India
  • Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ashok Kumar
  • Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rajender Kumar / Liza Goyal / Parveen Goel
  • Department of Veterinary Medicine, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sanjay Kumar
Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0045

Abstract

A sizeable Indian equine population is considered to be pre-immune carrier of Theileria equi infection. In this study we confirmed the presence of T. equi specific DNA in Hyalomma anatolicum ticks which were infested on sero-positive horses. Fifty two Indigenous horses were randomly selected from endemic areas and their blood and tick samples were collected. Tick salivary glands and blood samples were processed for separation of DNA and serum, respectively. Serum samples were analyzed by EMA-2ELISA and nine horses were found positive for T. equi specific antibodies. Species-specific primers were designed from EMA-2 gene of T. equi, so as to amplify 398 bp fragment in PCR. The gene fragment was amplified in PCR on the DNA samples (from blood) from these nine sero-positive horses. Corresponding six tick’s DNA samples collected from these nine seropositive animals were observed positive in PCR. Further, qPCR assay demonstrated presence of T. equi DNA in infected tick’s salivary glands, which was also confirmed by microscopic examination of infected acinar. This study concluded that Hyalomma anatolicum ticks infested on T. equi seropositive horses have sporozoite developmental stage in their salivary glands, which is an evidence for transmitting potential of these tick among Indian horse population.

Keywords: Babesia equi; Theileria equi; Tick; Hyalomma anatolicum; ELISA; PCR

References

  • Baldani C.D., Hilario E., Nakaghi A.C., Bertolini M.C., Machado R.Z. 2011. Production of recombinant EMA-1 protein and its application for the diagnosis of Theileria equi using an enzyme immunoassay in horses from Sao Paulo State, Brazil. Revista Brasileira de Parasitologia Veterinária, 20, 54-60Web of ScienceGoogle Scholar

  • Bashiruddin J.B., Camma C., Rebelo E. 1999. Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16S rRNA gene. Veterinary Parasitology, 84, 75-83CrossrefGoogle Scholar

  • Battsetseg B., Lucero S., Xuan X., Claveria F., Byambaa B., Battur B., Boldbaatar D., Batsukh Z., Khaliunaa T., Battsetseg G., Igarashi I., Nagasawa H., Fujisaki K. 2002. Detection of equine Babesia spp. gene fragments in Dermacentor nuttalli Olenev 1929 infesting mongolian horses, and their amplification in egg and larval progenies. Journal of Veterinary Medical Science, 64,727-30Google Scholar

  • Battsetseg B., Xuan X., Ikadai H., Bautista J.L., Byambaa B., Boldbaatar D., Battur B., Battsetseg G., Batsukh Z., Igarashi I., Nagasawa H., Mikami T., Fujisaki K. 2001. Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks. International Journal for Parasitology, 31, 384-6CrossrefPubMedGoogle Scholar

  • Boldbaatar D., Xuan X., Battsetseg B., Igarashi I., Battur B., Batsukh Z., Bayambaa B., Fujisaki K. 2005. Epidemiological study of equine piroplasmosis in Mongolia. Veterinary Parasitology, 127, 29-32Google Scholar

  • De Waal, D.T. 1992. Equine piroplasmosis: a review. British Veterinary Journal, 148, 6-14 Google Scholar

  • Friedhoff K.T. and Soule C. 1996. An account of equine babesiosis. Review Scientific et Technique Office Internationale des Epizooties, 15, 1191-1201Google Scholar

  • Geevarghese G., Fernandes S., Kulkarni S.M. 1997. A checklist of Indian ticks (Acari: Ixodoidea). The Indian Journal of Animal Sciences, 67, 566-574Google Scholar

  • Haque M., Jyoti, Singh N.K., Rath S.S. 2010. Prevalence of Theileria annulata infection in Hyalomma anatolicum anatolicum in Punjab state, India. Journal of Parasitic Diseases, 34, 48-51Google Scholar

  • Huang, X., Xuan X., Yokoyama N., Xu L., Suzuki H., Sugimoto C., Nagasawa H., Fujisaki K., Igarashi I. 2003. High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential in immunodiagnosis. Journal of Clinical Microbiology, 41, 1147-51Google Scholar

  • Hussain M.H., Saqib M., Raza F., Muhammad G., Asi M.N., Mansoor M.K., Saleem M., Jabbar A. 2014. Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab,Pakistan. Veterinary Parasitology, 202, 248-256Web of ScienceGoogle Scholar

  • Ikadai H., Sasaki M., Ishida H., Matsuu A., Igarashi I., Fujisaki K., Oyamada T. 2007. Molecular evidence of Babesia equi trans mission in Haemaphysalis longicornis. American Journal of Tropical Medicine and Hygiene, 76, 694-7Google Scholar

  • Katoh K., Misawa K., Kuma K., Miyata T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059-3066PubMedCrossrefGoogle Scholar

  • Kim C.M., Blanco L.B.C., Alhassan A., Iseki H., Yokoyama N., Xuan X., Igarashi I. 2008. Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples. Veterinary Parasitology, 151, 158-163Web of ScienceGoogle Scholar

  • Knowles D.P. Jr., Kappmeyer L.S., Stiller D., Hennager S.G., Perryman L.E. 1992. Antibody to a recombinant merozoite protein epitope identified horses infected with Babesia equi. Journal of Clinical Microbiology, 30, 3122-3126PubMedGoogle Scholar

  • Kumar S., Kumar R. 2007. Diagnosis of Babesia equi infection in equines: An update on the methods available. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 2, 1-14Google Scholar

  • Kumar S., Malhotra D.V., Sangwan A.K., Goel P., Kumar A., Kumar S. 2007. Infectivity rate and transmission potential of Hyalomma anatolicum anatolicum ticks for Babesia equi infection. Veterinary Parasitology, 144, 338-343Web of ScienceGoogle Scholar

  • Kumar S., Sugimoto C., Igarashi I. 2005. Equine babesiosis in Asia. In: Asian Parasitology - Toxoplasmosis and Babesiosis in Asia (Vol 4) Yano A, Nam HW, Anuar K, Shen J, Saito A and Igarashi I. Pub The Federation of Asian Parasitologist, Japan pp. 187-195Google Scholar

  • Kumar S., Kumar R., Gupta A.K., Yadav S.C., Goyal S.K., Khurana S.K., Singh R.K. 2013a. Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population. Veterinary Parasitology, 198, 10-17Web of ScienceGoogle Scholar

  • Kumar Y., Malhotra D.V., Nichani A.K., Dhar S., Kumar S. 2013b. Immunokinetics of Theileria equi specific antibodies A comparison in serial and single dilution ELISA antibody end titres. Turkish Journal of Veterinary and Animal Sciences, 37, 429-433Web of ScienceGoogle Scholar

  • Kumar S., Kumar R., Gupta A.K., Dwivedi S.K. 2008. Passive transfer of Theileria equi antibodies to neonate foals of immune tolerant mares. Veterinary Parasitology, 151, 80-85Web of ScienceGoogle Scholar

  • Lori A., Gabrielli S., Calderini P., Moretti A., Pietrobelli M., Tampieri M.P., Galuppi R., Cancrini G. 2010. Tick reservoirs for piroplasm in central and northern Italy. Veterinary Parasitology, 170, 291-296 Web of ScienceGoogle Scholar

  • Mehlhorn H., Schein E. 1988. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998. Parasitology Research, 84, 467-75Google Scholar

  • Nicolaiewsky T.B., Richter M.F., Lunge V.R., Cunha C.W., Delagostin O., Ikuta N., Fonseca A.S., da Silva S.S., Ozaki L.S. 2001. Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction. Veterinary Parasitology, 101, 9-21Google Scholar

  • Rampersad J., Cesar E., Campbell M., Samlal M., Ammons D.A. 2003. Field evaluation of PCR for the routine detection of Babesia equi in horses. Veterinary Parasitology, 114, 81-87Google Scholar

  • Sambrook J., Russell D.W. 2001 Molecular cloning a laboratory manual. Third ed. Cold Spring Harbour Laboratory Press, New York Google Scholar

  • Seo M.G., Yun S.H., Choi S.K., Cho G.J., Park Y.S., Kwon O.D., Cho K.H., Kim T.H., Jeong K.S., Park S.J., Kwon Y.S., Kwak D. 2011. Seroprevalence of equine piroplasms in the Republic of Korea. Veterinary Parasitology, 179, 224-6Web of ScienceGoogle Scholar

  • Tanaka T., Xuan X., Ikadai H., Igarashi I., Nagasawa H., Fujisaki K., Mikami T., Suzuki N. 1999. Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA. International Journal for Parasitology, 29, 1803-1808Google Scholar

  • Tiwari A., Singh N.K., Singh H., Jyoti, Bhat S.A., Rath S.S. 2013. Prevalence of Theileria annulata infection in Hyalomma anatolicum anatolicum collected from crossbred cattle of Ludhiana, Punjab. Journal of Parasitic Diseases. DOI: 10.1007/s12639-013-0279-4 CrossrefGoogle Scholar

  • Untergasser A., Nijveen N., Rao X., Bisseling T., Geurts R., Leunissen J.A.M. 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Research, 35, W71-W74. DOI: 10.1093/nar/gkm306Web of ScienceCrossrefGoogle Scholar

  • Xuan X., Larsen A., Ikadai H., Tanaka T., Igarashi I., Nagasawa H., Fujisaki K., Toyoda Y., Suzuki N., Mikami T. 2001. Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 705-9Google Scholar

  • Yin H., Lu W., Luo J. 1997. Babesiosis in China. Tropical Animal Health and Production, 4 Suppl, 11S-15S Google Scholar

About the article

Received: 2014-06-11

Revised: 2014-12-27

Accepted: 2015-01-21

Published Online: 2015-03-25

Published in Print: 2015-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0045.

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© 2015 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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