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Acta Parasitologica

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Volume 60, Issue 2


The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model

Ayelet Livneh
  • Pediatric Division, Assaf Harofeh Medical Center, Zerifin 70300, Israel, Affiliated to the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
  • Other articles by this author:
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/ Ilan Youngster
  • Pediatric Division, Assaf Harofeh Medical Center, Zerifin 70300, Israel, Affiliated to the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yossef El-On
  • Parasitology Laboratory, Soroka University Medical Center, Ben-Gurion University of the Negev, P.O.B. 653, Beer-Sheva 8410501, Israel
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Matitiahu Berkovitch
  • Pediatric Division, Assaf Harofeh Medical Center, Zerifin 70300, Israel, Affiliated to the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ibrahim Abu-Kishk
  • Corresponding author
  • Pediatric Division, Assaf Harofeh Medical Center, Zerifin 70300, Israel, Affiliated to the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
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  • Other articles by this author:
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Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/ap-2015-0048


We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.

Keywords: Leishmania major; hyperbaric oxygen therapy; mouse model; methylbenzethonium chloride; paromomycin sulphate


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About the article

Received: 2014-05-25

Revised: 2014-11-20

Accepted: 2014-12-01

Published Online: 2015-03-25

Published in Print: 2015-06-01

Citation Information: Acta Parasitologica, Volume 60, Issue 2, Pages 345–349, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0048.

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