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Acta Parasitologica

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Volume 60, Issue 3 (Sep 2015)

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Ophthalmology hospital wards contamination to pathogenic free living Amoebae in Iran

Zohreh Lasjerdi
  • Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Other articles by this author:
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/ Maryam Niyyati
  • Corresponding author
  • Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Email
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/ Jacob Lorenzo-Morales
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Tenerife, Canary Islands, Spain
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/ Ali Haghighi
  • Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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/ Niloofar Taghipour
  • Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Published Online: 2015-06-16 | DOI: https://doi.org/10.1515/ap-2015-0057

Abstract

The present study was conducted to determine the occurrence of potentially pathogenic free-living amoeba in ophthalmology wards in reference hospitals in Iran. Since an increasing number of Acanthamoeba Keratitis cases after eye surgery and eye trauma have been recently observed in this country, it could be possible that the disinfection procedures undertaken in the clinical setting may not have a good hygiene and disinfection procedures, hence the aim of this study. Therefore, 42 dust and biofilm samples were collected from different areas of ophthalmology wards and checked for the presence of FLA using morphological criteria, PCR based analysis and DNA sequencing. Of the 42 samples from dust and biofilm sources, 18(42.86%) isolates were found to contain FLA and 12(92.3%) isolates belonged to Acanthamoeba T4 genotype. Isolation of the pathogenic genotype T4 from medical instruments, including slit lamp in corneal wards, may be a threat for patients undergoing eye surgery in these wards. Other FLA isolated in this study included Acanthamoeba genotype T5, Vahlkampfia sp, Naegleria australiensis, Vermamoeba vermiformis and Echinamoeba exudans. To our knowledge, this is the first report of the presence of potentially pathogenic FLA in ophthalmology wards in Iran. Improved disinfection methods and monitoring of hospitals ward are thus necessary in this area in order to minimize the risk of infection in patients.

Keywords : Free-living amoeba; hospital; ophthalmology wards; Iran

References

  • Cengiz A.M., Harmis N., Stapleton F. 2000. Coincubation of Acanthamoeba castellanii with strains of Pseudomonas aeruginosa alters the survival of amoeba. Clinical Experimental Ophthalmology, 28, 191-193. DOI: 10.1046/j.1442-9071.2000.00291.x CrossrefGoogle Scholar

  • Chan L.L., Mak J.W., Low Y.T., Koh T.T., Ithoi I., Mohamed S.M. 2011. Isolation and characterization of Acanthamoeba spp. from air conditioners in Kuala Lumpur, Malaysia. Acta Tropica, 117, 23-30. DOI: 10.1016/j.actatropica.2010.09.004Web of ScienceCrossrefGoogle Scholar

  • Khan N.A., Jarroll E.L., Paget T.A. 2002. Molecular and Physiological Differentiation Between Pathogenic and Nonpathogenic Acanthamoeba. Current Microbiology, 45, 197-202. DOI: 10.1007/s00284-001-0108-3CrossrefGoogle Scholar

  • Khan N.A. 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiology Reviews, 30, 564-595. DOI: 10.1111/j.1574-6976.2006.00023.x CrossrefGoogle Scholar

  • Kong H.H., Chung D.I. 1996. PCR and RFLP variation of conserved region of small subunit ribosomal DNA among Acanthamoeba isolates assigned to either A. castellanii or A. polyphaga. Korean Journal Of Parasitology, 34, 127-134. DOI: org/10.3347/kjp.1996.34.2.127Google Scholar

  • Kuiper M.W., Valster R.M., Wullings B.A., Boonstra H., Smidt H., der Kooij D.V. 2006. Quantitative Detection of the Free-Living Amoeba Hartmannella vermiformis in Surface Water by Using Real-Time PCR.Applied and Environmental Microbiology, 72, 5750-5756. DOI: 10.1128/AEM.00085-06CrossrefGoogle Scholar

  • Maghsood A.H., Sissons J., Rezaeian M., Nolder D., Warhurst D., Khan N.A. 2005. Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. Journal of Medical Microbiology, 54, 755-759. DOI: 10.1099/jmm.0.45970-0CrossrefGoogle Scholar

  • Lasjerdi Z., Niyyati M., Haghighi A., Shahabi S., Tahvildar-biderouni F., Taghipour N., Eftekhar M., Nazemalhosseini Mojarad E. 2011. Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitology Research, 109, 575-580. DOI: 10.1007/s00 436-011-2288-5CrossrefWeb of ScienceGoogle Scholar

  • Ledee D.R., Iovieno A., Miller D., Mandal N., Diaz M., Fell J., Fini M.E., Alfonso E.C. 2009. Molecular identification of T4 and T5 genotypes in Acanthamoeba keratitis patients. Journal Of Clinical Microbiology, 47, 1458-1462. DOI: 10.1128/JCM. 02365-08Web of ScienceCrossrefGoogle Scholar

  • Lorenzo-Morales J., Ortega-Rivas A., Foronda P., Martiınez E., Valladares B. 2005. Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitology Research, 95, 273-277. DOI:10.1007/s00436-005-1301-2CrossrefGoogle Scholar

  • Lorenzo-Morales J., Ortega-Rivas A., Martiıınez E., Khoubbane M., Artigas P., Periago M.V., Foronda P., Abreu-Acosta N., Valladares B., Mas-Coma S. 2006. Acanthamoeba isolates belonging to T1, T2, T3, T4 and T7 genotypes from environmental freshwater samples in the Nile Delta region, Egypt. Acta Tropica, 100,63-69. DOI: 10.1016/j.actatropica.2006. 09.008CrossrefGoogle Scholar

  • Lorenzo-Morales J., Martinez-Carretero E., Batista N., Alvarez- Marin J., Bahaya Y.,Walochnik J., Valladares B. 2007. Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella. Parasitology Research, 102, 167-169Web of ScienceGoogle Scholar

  • Niyyati M., Lorenzo-Morales J., Rezaie S., Rahimi F., Mohebali M., Maghsood A.H., Motevalli-Haghi A., Martin-Navarro C.M., Farnia S.H., Valladares B., Rezaeian M. 2009a. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Experimental Parasitology, 121, 242-245. DOI: 10.1016/j.exppara.2008.11.003Web of ScienceCrossrefGoogle Scholar

  • Niyyati M., Lorenzo-Morales J., Rezaie S., Rahimi F., Mohebali M., Maghsood A.M. 2009b. Isolation and genotyping of potentially pathogenic Acanthamoeba strains from dust sources in Iran. Transactions Of The Royal Society Of Tropical Medicine and Hygiene, 103, 425-427. DOI: 10.1016/j.trstmh.2008.12.007Web of ScienceCrossrefGoogle Scholar

  • Niyyati M., Lorenzo-Morales J., Rezaeian M., Martin-Navarro C.M., Motevalli- Haghi A., Maciver S.K., Valladares B. 2009c. Isolation of Balamuthia mandrillaris from urban dust, free of known infectious involvement. Parasitology Research, 106,279-281.DOI: 10.1007/s00436-009-1592-9CrossrefWeb of ScienceGoogle Scholar

  • Niyyati M., Lorenzo-Morales J., Rezaie S., Rahimi F., Martin- Navarro C.M., Mohebali M., Maghsood A.H., Farnia S., Valladares B.,Rezaeian M. 2010. First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Experimental Parasitology, 126,89-90. DOI:10.1016/j.exppara.2009.10.009Web of ScienceCrossrefGoogle Scholar

  • Page F.C. 1988. A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, Ambleside, UK. ISBN 1871105021, 9781871105025Google Scholar

  • Pélandakis M., Pernin P. 2002. Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment. Applied and Environmental Microbiology, 68, 2061-2065. DOI: 10.1128/AEM.68.4.2061-2065.2002CrossrefGoogle Scholar

  • Sambrook J., Fristsch E.F., Maniatis T. 1989. Molecular cloning. A laboratory manual, 2nd ed. Cold Spring Harbour Laboratory Press. DOI: 10.1016/0003-2697(90)90595-Z CrossrefGoogle Scholar

  • Schroeder J.M., Booton G.C., Hay J., Niszl I.A., Seal D.V., Markus M.B., Fuerst P.A., Byers T.J. 2001. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. Journal Of Clinical Microbiology, 39, 1903-1911. DOI: 10.1128/JCM.39.5.1903-1911.2001CrossrefGoogle Scholar

  • Schuster F.L.,Visvesvara G.S. 2004. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. International Journal Of Parasitology, 34, 1001-1027. DOI: 10.1016/j.ijpara.2004.06.004CrossrefGoogle Scholar

  • Spanakos G., Tzanetou K., Miltsakakis D., Patsoula E., Malamou- Lada E., Vakalis N.C. 2006. Genotyping of pathogenic Acanthamoebae isolated from clinical samples in Greece-report of a clinical isolate presenting T5 genotype. Parasitology International, 55, 147-149. DOI: 10.1016/j.parint.2005.12.001CrossrefGoogle Scholar

  • Zegans M. E., Becker H.I., Budzik J. and O’Toole G. 2002. The role of bacterial biofilms in ocular infections. DNA and Cell Biology, 21, 415-420. DOI:10.1089/10445490260099700CrossrefGoogle Scholar

  • Ziak P., Ondriska F., Mrva M. 2003. Acanthamoeba keratitis after use of soft contact lenses. Case report (in Slovak). Czesk and Slovak Ophthalmology, 59, 352-358Google Scholar

About the article

Received: 2014-11-03

Revised: 2015-01-24

Accepted: 2015-03-04

Published Online: 2015-06-16

Published in Print: 2015-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0057.

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