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Acta Parasitologica

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Volume 60, Issue 3 (Sep 2015)

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Molecular phylogeny and ultrastructure of Myxobolus cf. cuneus, a parasite of patinga hybrid and Henneguya pseudoplatystoma, a parasite of pintado hybrid

Tiago Milanin
  • Corresponding author
  • Animal Biology Department, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, CEP 13083-970, Campinas, SP, Brazil
  • Email
  • Other articles by this author:
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/ Antônio Augusto Mendes Maia
  • Veterinary Medicine Department, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Rua Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Márcia Ramos Monteiro Silva
  • Veterinary Medicine Department, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Rua Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mateus Maldonado Carriero
  • Department of Genetics and Evolution, Universidade Federal de São Carlos (UFSCar), São Carlos, SP/Brazil, Rodovia Washington Luis, Km. 235, CEP 13565-905, São Carlos, SP, Brazil
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  • De Gruyter OnlineGoogle Scholar
/ Edson Aparecido Adriano
  • Animal Biology Department, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, CEP 13083-970, Campinas, SP, Brazil
  • Department of Biological Science, Universidade Federal de São Paulo, Rua Professor Artur Riedel, 275, Jardim Eldorado, CEP 09972-270 Diadema, SP, Brazil
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-16 | DOI: https://doi.org/10.1515/ap-2015-0061

Abstract

Through morphological, histopathological and ultrastructural analysis of Myxobolus cuneus Adriano, Arana et Cordeiro, 2006 and Henneguya pseudoplatystoma Naldoni, Arana, Maia, Ceccarelli, Tavares, Borges, Pozo et Adriano, 2009 were identified infecting pacu respectively (Piaractus mesopotamicus) and hybrid pintado (Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum) taken from Brazilian fish farms. The present study describes 18S rDNA sequencing of Myxobolus cf. cuneus infecting the spleen of farmed patinga, a hybrid fish resulting from the crossing of P. mesopotamicus x Piaractus brachypomus, and H. pseudoplatystoma found in farmed hybrid pintado from the state of Sao Paulo, Brazil. The study also provides new details of the host-parasite interface of M. cf. cuneus, which reveal that the plasmodial wall is composed of a single membrane connected to the plasmodium ectoplasm by numerous pinocytic canals. The plasmodia also displayed asynchronous development but had disporic pansporoblasts at different developmental stages; immature and mature spores were found at different depth levels of the plasmodium. Maximum likelihood phylogenetic analysis showed that M. cf. cuneus appeared as a sister species of Henneguya pellucida Adriano, Arana et Cordeiro, 2005 in a sub-clade composed mainly of myxosporean parasites of characiforms, and that H. pseudoplatystoma clustered in a sub-clade composed of Henneguya/Myxobolus spp. parasites of siluriform fish.

Keywords : Myxobolus; Henneguya; Phylogeny; 18S rDNA; Ultrastructure

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About the article

Received: 2014-10-14

Revised: 2015-01-23

Accepted: 2015-03-13

Published Online: 2015-06-16

Published in Print: 2015-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0061.

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