Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

More options …
Volume 60, Issue 3


Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers

Ivica Králová-Hromadová / Eva Bazsalovicsová / Aleksander W. Demiaszkiewicz
Published Online: 2015-06-16 | DOI: https://doi.org/10.1515/ap-2015-0077


The giant liver fluke, Fascioloides magna, is a veterinary important liver parasite of free living and domestic ruminants. This originally North American parasite was introduced along with its cervid hosts to Europe where it has established three permanent natural foci - in northern Italy, central and southern parts of the Czech Republic and the Danube floodplain forests. The first record on fascioloidosis in Poland originated from the Lower Silesian Forest in south-western Poland and since then an occurrence of F. magna in this country has not been documented. Recently, the parasitological examination of red deer (Cervus elaphus elaphus) from the Lower Silesian Wilderness (south-western Poland) revealed the presence of F. magna eggs. In order to determine the genetic interrelationships of the Polish giant liver fluke individuals, they were molecularly analyzed by mitochondrial cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) and compared with haplotypes of so far studied European populations of the parasite. The study revealed the genetic uniformity of F. magna specimens from Poland with part of individuals from the Czech natural focus.

Note: Nucleotide sequence data reported in this paper are available in the GenBankTM, EMBL and DDBJ databases under the accession numbers KP635008-9.

Keywords : Liver flukes; invasive species; mitochondrial haplotype; parasites of cervids


  • Almarza N. 1935. The liver fluke in sheep (in German). Description of new species. Infectious diseases and Hygiene of Pets, 47, 195-202Google Scholar

  • Apostolo C. 1996. The naturalistic aspects: flora, fauna and the environment. In: (Eds. M. Lupo, M. Paglieri, C. Apostolo, E. Vaccarino and M. Debernardi) La Mandria Storia e natura del Parco. Edizioni Eda, Torino, 67−98Google Scholar

  • Bazsalovicsová E., Králová-Hromadová I., Špakulová M., Reblánová M., Oberhauserová K. 2010. Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia, 47, 76-82. DOI: 10.2478/s11687-010-0011-1Web of ScienceCrossrefGoogle Scholar

  • Bazsalovicsová E., Králová-Hromadová I., Radvánszky J., Beck R. 2013. The origin of the giant liver fluke, Fascioloides magna (Trematoda: Fasciolidae) from Croatia determined by highresolution melting screening of mitochondrial cox1 haplotypes. Parasitology Research, 112, 2661-2666. DOI: 10.1007/ s00436-013-3433-0Web of ScienceCrossrefGoogle Scholar

  • Chroustová E., Hůlka J., Jaroš J. 1980. Prevence a terapie fascioloidózy skotu bithionosulfoxidem. Veterinární medicína, Praha, 25, 557-563. (in Czech) Google Scholar

  • Demiaszkiewicz A.W. 2014. Migrations and the introduction of wild ruminants as a source of parasite exchange and emergence of new parasitoses. Annals of Parasitology, 60, 25-30Google Scholar

  • Erhardová-Kotrlá B. 1971. The occurrence of Fascioloides magna (Bassi, 1875) in Czechoslovakia, Publishing House of the Czechoslovak Academy of Sciences, Academia, Prague, 155 pp.Google Scholar

  • Kašný M., Beran L., Siegelová V., Siegel T., Leontovyč R., Beránková K., Pankrác J., Kostáková M., Horák P. 2012. Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Veterinární Medicína, 57, 101-109Google Scholar

  • Králová-Hromadová I., Špakulová M., Horáčková E., Turčeková Ľ., Novobilský A., Beck R., Koudela B., Marinculić A., Rajský D., Pybus M. 2008. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology, 94, 58-67. DOI: 10.1645/GE-1324.1CrossrefWeb of ScienceGoogle Scholar

  • Králová-Hromadová I., Bazsalovicsová E., Štefka J., Špakulová M., Vávrová S., Szemes T., Tkach V., Pybus M. 2011. Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. International Journal for Parasitology, 41, 373-383. DOI: 10.1016/j.ijpara.2010.10.010CrossrefWeb of ScienceGoogle Scholar

  • Majoros G., Sztojkov V. 1994. Appearance of the large American liver fluke Fascioloides magna (Bassi, 1875) (Trematoda: Fasciolata) in Hungary. Parasitologia Hungarica, 27, 27−38.Google Scholar

  • Marinculić A., Džakula N., Janicky Z., Lućinger S., Živićniak T. 2002. Appearance of American liver fluke (Fascioloides magna Bassi, 1875) in Croatia - a case report. Veterinarski Archiv, 72, 319−325Google Scholar

  • Mariković D., Kukolj V., Aleksić-Kovačević S., Jovanović M., Knežević M. 2013. The role of hepatic myofibroblasts in liver cirrhosis in fallow deer (Dama dama) naturally infected with giant liver fluke (Fascioloides magna). BMC Veterinary Research 9, 45. DOI:10.1186/1746-6148-9-45Web of ScienceCrossrefGoogle Scholar

  • Novobilský A., Horáčková E., Hirtová L., Modrý D., Koudela B. 2007. The giant liver fluke Fascioloides magna (Bassi, 1875) in cervids in the Czech Republic and potential of its spreading to Germany. Parasitology Research, 100, 549-553. DOI: 10.1007/s00436-006-0299-4Web of SciencePubMedCrossrefGoogle Scholar

  • Pybus M.J. 2001. Liver flukes. In: (Eds. W.M. Samuel, M.J. Pybus and A.A. Kocan) Parasitic diseases of wild mammals, 2nd edition. Iowa State Press, Iowa City, 121-149Google Scholar

  • Pyziel A.M., Demiaszkiewicz A.W., Kuligowska I. 2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from South-Western Poland (Lower Silesian wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523-525. DOI: 10.2478/ pjvs-2014-0071CrossrefWeb of ScienceGoogle Scholar

  • Rajský D., Patus A., Bukovjan K. 1994. Prvý nález Fascioloides magna (Bassi, 1875) na Slovensku. Slovenský Veterinársky Časopis, 19, 29−30. (in Slovak) Google Scholar

  • Salomon S. 1932. Fascioloides magna bei deutschem Rotwild. Berliner Tierärztliche Wochenschrift, 48, 627-628. (in German) Google Scholar

  • Skog A., Zachos F.E., Rueness E.K., Feulner P.G.D., Mysterud A., Langvatn R., Lorenzini R., Hmwe S.S., Lehoczky I., Hartl G.B., Stenseth N.C., Jakobsen K.S. 2009. Phylogeography of red deer (Cervus elaphus) in Europe. Journal of Biogeography, 36, 66-77. DOI: 10.1111/j.1365-2699.2008.01986.x CrossrefWeb of ScienceGoogle Scholar

  • Slusarski W. 1955. Studia nad europejskimi przedstawicielami przywry Fasciola magna (Bassi, 1875) Stiles, 1894. Acta Parasitologica Polonica, 3, 1-59. (in Polish) Google Scholar

  • Ullrich K. 1930. Über das Vorkommen von seltenen oder wenig bekannten Parasiten der Säugetiere und Vögel in Böhmen und Mähren. Prager Archiv Tiermedicine, 10 A (1/2), 19−43. (in German) Google Scholar

  • Werle E., Schneider C., Renner M., Volker M., Fiehn W. 1994. Convenient singlestep, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354-4355 CrossrefGoogle Scholar

  • Winkelmayer R., Prosl H. 2001. Riesenleberegel − jetzt auch bei uns? Österreichisches Weidwerk, 3, 42−44. (in German)Google Scholar

About the article

Received: 2015-01-16

Revised: 2015-02-23

Accepted: 2015-04-18

Published Online: 2015-06-16

Published in Print: 2015-09-01

Citation Information: Acta Parasitologica, Volume 60, Issue 3, Pages 544–547, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0077.

Export Citation

© W. Stefański Institute of Parasitology, PAS.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

L. Juhásová, I. Králová-Hromadová, M. Zeleňáková, P. Blišťan, and E. Bazsalovicsová
Helminthologia, 2017, Volume 54, Number 2
Ludmila Juhásová, Ivica Králová-Hromadová, Eva Bazsalovicsová, Gabriel Minárik, Jan Štefka, Peter Mikulíček, Lenka Pálková, and Margo Pybus
Parasites & Vectors, 2016, Volume 9, Number 1

Comments (0)

Please log in or register to comment.
Log in