Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

More options …
Volume 60, Issue 3


Molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, Scomber japonicus

Niichiro Abe
  • Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mitsuru Okamoto
  • Shimane Prefectural Fisheries Technology Center, 25-1 Setogashima-cho, Hamada, Shimane 697-0051, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-16 | DOI: https://doi.org/10.1515/ap-2015-0080


Didymozoids found in the muscles of marine fish are almost always damaged because they are usually found after being sliced. Therefore, identifying muscle-parasitizing didymozoids is difficult because of the difficulty in collecting non-damaged worms and observing their organs as key points for morphological identification. Moreover, muscle-parasitizing didymozoids are not easily found because they parasitize at the trunk muscles. Therefore, muscle-parasitizing didymozoid classification has not progressed because there are few opportunities to detect them. Our recent report was the first to describe the usefulness of sequencing analysis for discrimination among muscle-parasitizing didymozoids. Recently, we found a didymozoid in the trunk muscle of a chub mackerel Scomber japonicus. The present study genetically compares the present isolate with other muscle-parasitizing didymozoids. The present isolate differs markedly from the previously unidentified didymozoid from an Atlantic mackerel S. scombrus by phylogenetic analysis of 18S rDNA. It also differs from other muscle-parasitizing didymozoids from other host species based on phylogenetic analyses of 18S, 28S rDNAs, and coxI loci. These results suggest that sequencing analysis is useful for the discrimination of muscle-parasitizing didymozoids. Combining the present data with earlier data for sequencing analysis, muscle-parasitizing didymozoids from seven marine fish species were classified as seven species. We proposed appellations for six distinct muscle-parasitizing didymozoids for future analysis: sweetlips fish type from Diagramma pictum and Plectorhinchus cinctus, red sea bream type from Pagrus major, flying fish type from Cypselurus heterurus, Atlantic mackerel type from Scomber scombrus, chub mackerel type from S. japonicus, and purple rockcod type from Epinephelus cyanopodus.

Keywords : Classification; Didymozoidae; didymozoid; Scomber japonicus; trunk muscle


  • Abe N., Okamoto M., Maehara T. 2014. Molecular characterization of muscle-parasitizing didymozoids in marine fishes. Acta Parasitologica, 59, 354-358. DOI: 10.2478/s11686-014-0234-2Web of ScienceCrossrefGoogle Scholar

  • Cribb T.H., Williams A. 1992. Gonapodasmius williamsoni sp. n. (Digenea: Didymozoidae) from the pink snapper, Pagrus auratus (Teleostei: Sparidae) in western Australia. Journal of Helminthological Society of Washington, 59, 153-158Google Scholar

  • Ishii N. 1935. Studies of the family Didymozooidae (Monticelli, 1888). Japanese Journal of Zoology, 6, 279-335Google Scholar

  • Mladineo I., Bott N.J., Nowak B.F., Block B.A. 2010. Multilocus phylogenetic analyses reveal that habitat selection drives the speciation of Didymozoidae (Digenea) parasitizing Pacific and Atlantic bluefin tunas. Parasitology, 137, 1013-1025. DOI: 10.1017/S0031182009991703Web of ScienceCrossrefGoogle Scholar

  • Momoyama K., Tensha K. 2006. Ugly-looking parasitic infections and other abnormalities of wild fish and shellfish caught in the coastal or inland waters around or in Yamaguchi Prefecture. Bulletin of Yamaguchi Prefectural Fishery Research Center, 4, 143-161Google Scholar

  • Nakajima K., Sugiyama T., Egusa S. 1974. A trematodes, Gonapodasmius okushimai ISHII, found in the muscle of red sea bream, Pagrosomus major. Fish Pathology, 8, 175-176Google Scholar

  • Okamoto M. 2011. The parasites of fishes and shellfishes caught in the southwestern Sea of Japan off Shimane Prefecture. Report of Shimane Prefectural Fishery Technology Center, 3, 55-68Google Scholar

  • Okushima A. 1921. A new aschelminth parasitic in muscle of Pagrosomus major. Fukuoka Ika Daigaku Zasshi, 14, 463-470Google Scholar

  • Olson P.D., Cribb T.H., Tkach V.V., Bray R.A., Littlewood D.T.J. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology, 33, 733-755. DOI: 10.1016/S0020-7519(03)00049-3CrossrefGoogle Scholar

  • Pascual S., Abollo E., Azevedo C. 2006. Host-parasite interaction of a muscle-infecting didymozoid in the Atlantic mackerel Scomber scombrus L. ICES Journal of Marine Science, 63, 169-175. DOI: 10.1016/j.icesjms.2005.08.010CrossrefGoogle Scholar

  • Pozdnyakov S.E., Gibson D.I. 2008. Family Didymozooidae Monticelli, 1888. In Keys to the Trematoda Vol. 3, Bray R.A., Gibson D.I., Jones A. (Eds.). CAB International and Natural Museum, London, U.K., pp. 848Google Scholar

  • Yagi K., Terashima H., Takano K., Uraguchi K. 2007. Molecular identification of animal foreign substances in foods and food products: Identification of a parasite in the muscle of Crescent sweetlips Plectorhinchus cinctus. Report of Hokkaido Institute of Public Health, 57, 97-99Google Scholar

About the article

Received: 2014-10-10

Revised: 2015-03-23

Accepted: 2015-03-26

Published Online: 2015-06-16

Published in Print: 2015-09-01

Citation Information: Acta Parasitologica, Volume 60, Issue 3, Pages 557–562, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0080.

Export Citation

© W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in