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Acta Parasitologica

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Volume 60, Issue 4 (Dec 2015)

Issues

Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo

Roger D. Wumba
  • Corresponding author
  • Department of Tropical Medicine, University Clinic of Kinshasa, Faculty of Medicine, University of Kinshasa, Kinshasa, the Democratic Republic of Congo
  • Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Josué Zanga
  • Department of Tropical Medicine, University Clinic of Kinshasa, Faculty of Medicine, University of Kinshasa, Kinshasa, the Democratic Republic of Congo
  • Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kennedy M. Mbanzulu
  • Department of Tropical Medicine, University Clinic of Kinshasa, Faculty of Medicine, University of Kinshasa, Kinshasa, the Democratic Republic of Congo
  • Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Madone N. Mandina
  • Division of Infectious Diseases, Department of Internal Medicine, University Hospital of Kinshasa, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Aimé K. Kahindo
  • Department of Tropical Medicine, University Clinic of Kinshasa, Faculty of Medicine, University of Kinshasa, Kinshasa, the Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Michel N. Aloni
  • Division of Hemato-oncology and Nephrology, Department of Paediatrics, University Hospital of Kinshasa, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mathilde B. Ekila
  • Division of Infectious Diseases, Department of Internal Medicine, University Hospital of Kinshasa, School of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-09-25 | DOI: https://doi.org/10.1515/ap-2015-0090

Abstract

Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.

Keywords: Cryptosporidium spp; molecular biology; HIV/AIDS; Kinshasa; Democratic Republic of Congo; Africa

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About the article

Received: 2014-12-11

Revised: 2015-04-05

Accepted: 2015-05-08

Published Online: 2015-09-25

Published in Print: 2015-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0090.

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