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Acta Parasitologica

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Volume 60, Issue 4 (Dec 2015)

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Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from southern Poland

Joanna K. Strzelczyk
  • Corresponding author
  • Chair and Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808 Zabrze, Poland
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/ Jadwiga Gaździcka
  • Department of Parasitology, School of Pharmacy, Medical University of Silesia in Katowice, Jedności 8 Str., 41-200 Sosnowiec, Poland
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/ Piotr Cuber
  • Department of Parasitology, School of Pharmacy, Medical University of Silesia in Katowice, Jedności 8 Str., 41-200 Sosnowiec, Poland
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/ Marek Asman
  • Department of Parasitology, School of Pharmacy, Medical University of Silesia in Katowice, Jedności 8 Str., 41-200 Sosnowiec, Poland
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/ Gizela Trapp
  • Chair and Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808 Zabrze, Poland
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/ Karolina Gołąbek
  • Chair and Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808 Zabrze, Poland
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/ Marzena Zalewska-Ziob
  • Chair and Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808 Zabrze, Poland
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/ Magdalena Nowak-Chmura
  • Pedagogical University of Cracow, Institute of Biology, Department of Invertebrate Zoology and Parasitology Podbrzezie 1 Str., 31-054 Cracow, Poland
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/ Krzysztof Siuda
  • Pedagogical University of Cracow, Institute of Biology, Department of Invertebrate Zoology and Parasitology Podbrzezie 1 Str., 31-054 Cracow, Poland
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/ Andrzej Wiczkowski
  • Chair and Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Jordana 19 Str., 41-808 Zabrze, Poland
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/ Krzysztof Solarz
  • Department of Parasitology, School of Pharmacy, Medical University of Silesia in Katowice, Jedności 8 Str., 41-200 Sosnowiec, Poland
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Published Online: 2015-09-25 | DOI: https://doi.org/10.1515/ap-2015-0095

Abstract

In 2008-2011 ticks were collected from southern Poland. Out of 6336 individuals collected and identified as Ixodes ricinus, 768 (2 larvae, 84 nymphs, 417 females, 265 males) were included in molecular study. The aim of this study was to investigate the prevalence and types of genospecies of Borrelia burgdorferi sensu lato in ticks. The polymerase chain reaction (PCR) was applied to detect the presence of pathogens in ticks. Subsequently the amplified DNA was digested with TasI enzyme. The infection rate was 15% (116) of examined ticks. PCR-RFLP analysis allowed distinguishing three genospecies of B. burgdorferi s.l.: B. burgdorferi sensu stricto, B. afzelii, and B. garinii. RFLP analyses of 116 positive samples revealed 96 (83%) monoinfections and 13 (11%) coinfections, whereas unidentified genospecies were present in 7 (6%) of positive samples. In the case of monoinfections, B. burgdorferi s.s. was the predominant species of pathogen in infected ticks - 61.4%. Other genospecies: B. garinii and B. afzelii were detected in 22.9% and 15.6% of the samples, respectively. To sum up, 15 % of ticks were infected by B. burgdorferi s.l which increases the risk of human infections in the recreational areas of southern Poland. Furthermore, there is a need to increase public awareness and implement more preventive measures concerning Lyme disease.

Keywords: Borrelia burgdorferi sensu lato; Ixodes ricinus; ticks; genospecies; Poland

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About the article

Received: 2014-08-28

Revised: 2015-05-07

Accepted: 2015-05-13

Published Online: 2015-09-25

Published in Print: 2015-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0095.

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