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Acta Parasitologica

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Volume 60, Issue 4 (Dec 2015)

Issues

Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt

Ahmed El-Morsey
  • Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, 33 El Bohouth St. (former El-Tahrir St.), Dokki, P.O.12622, Giza, Egypt
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mahmoud El-Seify / Abdel-Razik Y. Desouky / Mohamed M. Abdel-Aziz
  • Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, 33 El Bohouth St. (former El-Tahrir St.), Dokki, P.O.12622, Giza, Egypt
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Khaled Mohamed El-Dakhly
  • Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Samy Kasem / Walied Abdo
  • Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, KafrElsheikh University, Egypt
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mohie Haridy
  • Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hiroki Sakai
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tokuma Yanai
  • Corresponding author
  • Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
  • Email
  • Other articles by this author:
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Published Online: 2015-09-25 | DOI: https://doi.org/10.1515/ap-2015-0098

Abstract

A single morphologic type of Sarcocystis cysts found in two out of 43 examined common coots, Fulica atra, is considered to represent a new species for which the name Sarcocystis atraii n. sp. is proposed and its description is provided. Coots were hunted from the vicinity of Brolos Lake located at KafrElsheikh province, Egypt. The structural morphology of the revealed sarcocysts was described using light and transmission electron microscopy. Sarcocysts were found in the leg and thigh muscles. The cysts were microscopic and measured 165−850 μm in length × 50−85 μm in width. Histologically; the sarcocyst wall was wavy and had minute undulations. Ultrastructurally, it measured 1−3 μm in thickness and possessed many mushroom-like villar protrusions sometimes originating from other mushroom-like villar protrusions that measured approximately 0.5−2 μm in length and up to 2 μm in width, with the presence of electron dense ground substance of 300 nm to 1 μm thick. The bradyzoites were elongated, banana-shaped and measured 7.5−14 × 1.5−2.5 μm, with centrally or terminally located nuclei. The ultrastructural features of the cyst wall belonged to type 24. On the basis of sequencing and phylogenic analyses for 18S rRNA , 28S rRNA genes and ITS-1 region; S. atraii n. sp. is considered a genetically distinct species, being most closely related to avian Sarcocystis spp. whose definitive hosts are predatory mammals.

Keywords: Sarcocystis atraii n.sp.; common coot (Fulica atra); morphology; molecular identification; phylogeny

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About the article

Received: 2015-03-09

Revised: 2015-04-14

Accepted: 2015-05-22

Published Online: 2015-09-25

Published in Print: 2015-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2015-0098.

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