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Acta Parasitologica

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Volume 61, Issue 1 (Jan 2016)

Issues

Expression analysis of viscerotropic leishmaniasis gene in Leishmania species by real-time RT-PCR

Hossein Reisi Nafchi
  • Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Other articles by this author:
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/ Elham Kazemi-Rad
  • Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
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/ Mehdi Mohebali
  • Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
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/ Reza Raoofian / Niloofar Bavarsad Ahmadpour
  • Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
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/ Mohammad Ali Oshaghi
  • Departments of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
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/ Homa Hajjaran
  • Corresponding author
  • Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Email
  • Other articles by this author:
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Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/ap-2016-0011

Abstract

Viscerotropic leishmaniasis (VTL) is a parasitic disease with non-specific manifestations caused by Leishmania tropica. Specific antigens produced by Viscerotropic leishmaniasis gene have been used for diagnosis of VTL. The aim of this study was to compare the expression level of VTL gene among the viscerotropic L. tropica isolates (n: 3) and visceral L. infantum isolates (n: 4). Also, the expression level was compared in L. tropica (n: 21) and L. major (n: 8) isolates, the main causes of cutaneous leishmaniasis in Iran by real time-RT-PCR. Results showed viscerotropic leishmaniasis gene was expressed in all 3 species; L. tropica, L. major and L. infantum. The most expression rate was in L. tropica and L. major as the cutaneous species and the lowest in visceral isolates including L. infantum and viscerotropic L. tropica strains respectively.

Conclusion: Results revealed that VTL gene can play an important role in visceralization process of L. tropica although there are other mechanisms to keep parasite visceralized. According to these primary results, increased the expression level of VTL gene probably could contribute to inhibit the invasive behavior of Leishmania parasites. However, more experimental researches are needed to confirm this idea.

Keywords : Viscerotropic leishmanisis gene; real time RT- PCR; Leishmania species; Iran

References

  • Alvar J., Velez I.D., Bern C., Herrero M., Desjeux P., Cano J., Jannin J., Den Boer M., Team W.L.C. 2012. Leishmaniasis worldwide and global estimates of its incidence. PloS one, 7, e35671. DOI: 10.1371/journal.pone.0035671CrossrefGoogle Scholar

  • Colebunders R., Depraetere K., Verstraeten T., Lambert J., Hauben E., Van Marck E., Maurer T., Bañuls A.L., Dujardin J.C. 1999. Unusual cutaneous lesions in two patients with visceral leishmaniasis and HIV infection. Journal of the American Academy of Dermatology, 41, 847-850. DOI: 10.1016/S0190-9622(99)70342-7CrossrefGoogle Scholar

  • Dillon D.C., Day C.H., Whittle J.A., Magill A.J., Reed S.G. 1995. Characterization of a Leishmania tropica antigen that detects immune responses in Desert Storm viscerotropic leishmaniasis patients. Proceedings of the National Academy of Sciences, 92, 7981-7985. DOI:10.1073/pnas.92.17.798CrossrefGoogle Scholar

  • Gasser R.A., Magill A.J., Oster C.N., Tramont E.C. 1991. The threat of infectious disease in Americans returning from Operation Desert Storm. New England Journal of Medicine, 324, 859-864. DOI: 10.1056/NEJM199103213241229CrossrefGoogle Scholar

  • Hajjaran H., Mohebali M., Akhavan A., Taheri A., Barikbin B., Soheila N.S. 2013a. Unusual presentation of disseminated cutaneous leishmaniasis due to Leishmania major Case reports of four Iranian patients. Asian Pacific Journal of Tropical Medicine, 6, 333-336. DOI: 10.1016/S1995-7645(13) 60066-8Web of ScienceCrossrefGoogle Scholar

  • Hajjaran H., Mohebali M., Mamishi S., Vasigheh F., Oshaghi M.A., Naddaf S.R., Teimouri A., Edrissian G.H., Zarei Z. 2013b. Molecular identification and polymorphism determination of cutaneous and visceral leishmaniasis agents isolated from human and animal hosts in Iran. BioMed Research International, Article ID: 789326. 7 Pages. DOI: 10.1155/ 2013/789326Web of ScienceGoogle Scholar

  • Hajjaran H., Mohebali M., Teimouri A., Oshaghi M.A., Mirjalali H., Kazemi-Rad E., Shiee M.R., Naddaf S.R. 2014. Identification and phylogenetic relationship of Iranian Strains of various Leishmania species isolated from cutaneous and visceral cases of Leishmaniasis based on N acetyl glucosamine-1-phosphate transferase gene. Infection, Genetics and Evolution, 26, 203-212. DOI: 10.1016/j.meegid.2014.05.026Web of ScienceCrossrefGoogle Scholar

  • Hajjaran H., Mohebali M., Zarei Z., Edrissian G.H. 2007. Leishmania tropica: Another etiological agent of canine visceral leishmaniasis in Iran. Iranian Journal of Public Health, 36, 85-88.Google Scholar

  • Jafari S., Hajiabdolbaghi M., Mohebali M., Hajjaran H., Hashemian H. 2010. Disseminated leishmaniasis caused by Leishmania tropica in HIV-positive patients in the Islamic Republic of Iran. Eeastern Meditteranean Health Journal, 16, 340-343. PMID: 20795452Google Scholar

  • Kazemi-Rad E., Mohebali M., Khadem-Erfan M.B., Hajjaran H., Hadighi R., Khamesipour A., Rezaie S., Saffari M., Raoofian R., Heidari M. 2013a. Overexpression of ubiquitin and amino acid permease genes in association with antimony resistance in Leishmania tropica field isolates. The Korean Journal of Parasitology, 51, 413-419. DOI: 10.3347/kjp.2013.51.4.413CrossrefWeb of ScienceGoogle Scholar

  • Kreutzer R.D., Grogl M., Neva F.A., Fryauff D.J., Magill A.J., Aleman-Munoz M.M. 1993. Identification and genetic comparison of leishmanial parasites causing viscerotropic and cutaneous disease in soldiers returning from Operation Desert Storm. The American Journal of Tropical Medicine and Hygiene, 49, 357-363. PMID: 8372957Google Scholar

  • Livak K.J., Schmittgen T.D. 2001. Analysis of relative gene expression data using Real Time quantitative PCR and the 2 Delta delta ct Method. Methods, 25, 402-408. DOI: 10.1006/meth. 2001.1262CrossrefGoogle Scholar

  • Livneh A., Youngster I., El-On Y., Berkovitch M., Abu-Kishk I. 2015. The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model. Acta Parasitol, 60, 345-349. DOI: 10.1515/ap-2015-0048Web of ScienceCrossrefGoogle Scholar

  • Magill A.J., Grogl M., Gasser R.A.Jr., Sun W., Oster C.N. 1993. Visceral infection caused by Leishmania tropica in veterans of Operation Desert Storm. New England Journal of Medicine, 328, 1383-1387. DOI: 10.1056/NEJM199305133 281904CrossrefGoogle Scholar

  • Mohebali M., Malmasi A., Hajjaran H., Jamshidi S., Akhoundi B., Rezaei M., Janitabar S., Zarei H., Charehdar S. 2011. Disseminated leishmaniasis caused by Leishmania tropica in a puppy from Karaj, Central Iran. Iranian Journal of Parasitology, 6, 69-73. PMCID: PMC3279879Google Scholar

  • Mondal S., Roy J.J., Bera T. 2014. Generation of adenosine triphosphate in Leishmania donovani amastigote forms. Acta Parasitologica, 59, 11-6. DOI: 10.2478/s11686-014-0203-9Myler P.J., Fasel N. 2008. Leishmania: after the genome. Caister Academic Press, Horizon Scientific Press CrossrefGoogle Scholar

  • Peacock C.S., Seeger K., Harris D., Murphy L., Ruiz J.C., Quail M.A., Peters N., Adlem E., Tivey A., Aslett M. 2007. Comparative genomic analysis of three Leishmania species that cause diverse human disease. Nature Genetics, 39, 839-847. DOI: 10.1038/ng2053Web of ScienceCrossrefGoogle Scholar

  • Pfaffl M.W., Horgan G.W., Dempfle L. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30, e36. DOI: 10.1093/nar/30.9.e36 CrossrefGoogle Scholar

  • Raja K.M., Khan A.A., Hameed A., Rahman S.B. 1998. Unusual clinical variants of cutaneous leishmaniasis in Pakistan. British Journal of Dermatology, 139, 111-113. DOI: 10. 1046/j.1365-2133.1998.02325.x CrossrefGoogle Scholar

  • World Health Organization (WHO) authors. 2010. Control of the leishmaniases: report of a meeting of the WHO Expert Commitee on the Control of Leishmaniases. WHO Technical Report Series 949, Geneva, Switzerland. pp. 1-187 Google Scholar

About the article

Received: 2015-01-01

Revised: 2015-06-20

Accepted: 2015-09-15

Published Online: 2015-12-30

Published in Print: 2016-01-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0011.

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