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Acta Parasitologica

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Volume 61, Issue 1 (Jan 2016)

Issues

In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis

Mohd Saeed
  • Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Ha’il, Ha’il, PO Box 2440, Kingdom of Saudi Arabia
  • Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mohd Adnan
  • Corresponding author
  • Department of Clinical Nutrition, College of Applied Medical Sciences, University of Ha’il, Ha’il, PO Box 2440, Kingdom of Saudi Arabia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Saif Khan
  • Department of Clinical Nutrition, College of Applied Medical Sciences, University of Ha’il, Ha’il, PO Box 2440, Kingdom of Saudi Arabia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eyad Al-Shammari
  • Department of Clinical Nutrition, College of Applied Medical Sciences, University of Ha’il, Ha’il, PO Box 2440, Kingdom of Saudi Arabia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Huma Mustafa
Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/ap-2016-0015

Abstract

Lymphatic filariasis (LF) is a chronic disease and is caused by the parasites Wuchereria bancrofti (W. bancrofti), Brugia malayi (B. malayi) and Brugia timori (B. timori). In the present study, Setaria cervi (S. cervi), a bovine filarial parasite has been used. Previously, it has been reported that the S. cervi shares some common proteins and antigenic determinants with that of human filarial parasite. The larval stages of filarial species usually cannot be identified by classical morphology. Hence, molecular characterization allows the identification of the parasites throughout all their developmental stages. The genomic DNA of S. cervi adult were isolated and estimated spectrophotometrically for the quantitative presence of DNA content. Screening of DNA sequences from filarial DNA GenBank and Expressed Sequence Tags (EST’s) were performed for homologous sequences and then multiple sequence alignment was executed. The conserved sequences from multiple sequence alignment were used for In Silico primer designing. The successfully designed primers were used further in PCR amplifications. Therefore, in search of a promising diagnostic tool few genes were identified to be conserved in the human and bovine filariasis and these novel primers deigned may help to develop a promising diagnostic tool for identification of lymphatic filariasis.

Keywords : Diagnostic tool; lymphatic filariasis; bovine filarial parasite; Setaria cervi

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About the article

Received: 2014-04-13

Revised: 2015-07-09

Accepted: 2015-09-24

Published Online: 2015-12-30

Published in Print: 2016-01-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0015.

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