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Acta Parasitologica

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1896-1851
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Volume 61, Issue 1 (Jan 2016)

Issues

First report of Cystoisospora belli parasitemia in a patient with acquired immunodeficiency syndrome

Jorge Néstor Velásquez / Cecilia Alicia di Risio / Cristina Beatriz Etchart / Agustín Víctor Chertcoff
  • Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina
  • Other articles by this author:
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/ Mónica Gabriela Nigro
  • Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María Laura Pantano
  • Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina
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/ Bibiana Alba Ledesma
  • Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina
  • Other articles by this author:
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/ Natalia Vittar / Silvana Carnevale
  • Corresponding author
  • Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina
  • Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
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  • Other articles by this author:
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Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/ap-2016-0023

Abstract

Cystoisospora belli in patients with the acquired immunodeficiency syndrome (AIDS) has been described as cause of chronic diarrhea and disseminated cystoisosporosis. Diagnosis of intestinal cystoisosporosis can be achieved at the tissue level in the villus epithelium of the small bowel. Disseminated cystoisosporosis is diagnosed by microscopy identification of unizoite tissue cysts in the lamina propria of the intestine. We report a case of disseminated cystoisosporosis in a human immunodeficiency virus (HIV)-infected patient with detection of parasitemia. We studied a 39-year old patient with AIDS and chronic diarrhea by analysis of stool and duodenal biopsy samples. Blood samples were also collected and examined by light microscopy and molecular techniques for C. belli DNA detection. The unizoite tissue cyst stages were present in the lamina propria, with unsporulated oocysts in feces. Zoites were present in blood smears and DNA of C. belli was detected in blood samples. Our study identified a new stage in the life cycle of C. belli. Detection of parasitemia is a novel and noninvasive tool for diagnosis of disseminated cystoisosporosis.

Keywords : Cystoisospora belli; parasitemia; AIDS; unizoite tissue cyst; PCR

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About the article

Received: 2015-06-29

Revised: 2015-09-09

Accepted: 2015-10-13

Published Online: 2015-12-30

Published in Print: 2016-01-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0023.

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