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Acta Parasitologica

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Volume 61, Issue 2 (Jun 2016)

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Validation of the TrichinEasy® digestion system for the detection of Anisakidae larvae in fish products

Gaetano Cammilleri
  • Corresponding author
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Michele Chetta
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Antonella Costa
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Stefania Graci
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Rosaria Collura
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Maria Drussilla Buscemi
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Maria Cusimano
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Angelina Alongi
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Deborah Principato
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Giuseppe Giangrosso
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Antonio Vella
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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/ Vincenzo Ferrantelli
  • Centro di Referenza Nazionale per le Anisakiasi (C.Re.N.A) Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, Italy
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Published Online: 2016-03-30 | DOI: https://doi.org/10.1515/ap-2016-0048

Abstract

Anisakis and other parasites belonging to the Anisakidae family are organisms of interest for human health, because of their high zoonotic potential. Parasites belonging to this family can cause Anisakiasis, a parasitological disease caused by the ingestion of raw, infested fish products. Furthermore, evidence from the EFSA (European Food Safety Authority; EFSA 2010) has highlighted the allergological potential of nematodes belonging to the Anisakis genre. The detection and identification of Anisakidae larvae in fish products requires an initial visual inspection of the fish sample, as well as other techniques such as candling, UV illumination and artificial digestion. The digestion method consists of the simulation of digestive mechanics, which is made possible by the utilization of HCl and pepsin, according to EC Regulation 2075/2005. In this study, a new Anisakidae larvae detection method using a mechanical digestion system called Trichineasy® was developed. A total of 142 fish samples, belonging to 14 different species, were examined to validate the method. A reaction mixture with 100 g of sample, 10 g of pepsin (1:10000 NF) and 50 ml of 10% HCl at 36 ± 1°C for 20 minutes was evaluated to be the best condition for the digestion of fish samples. These parameters have also allowed the detection of viable larvae after digestion. The results confirm this instrumentation as a valuable and safe tool for the detection of Anisakidae larvae in fishery products.

Keywords: Anisakidae nematodes; artificial digestion; instrument validation

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About the article

Received: 2015-08-12

Revised: 2015-11-04

Accepted: 2015-12-14

Published Online: 2016-03-30

Published in Print: 2016-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0048.

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