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Acta Parasitologica

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1896-1851
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Volume 61, Issue 2 (Jun 2016)

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Prevalence and genotypes of Enterocytozoon bieneusi in sika deer in Jilin province, Northeastern China

Xiao-Xuan Zhang
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Wei Cong
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Guo-Hua Liu
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Xiao-Ting Ni
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jian-Gang Ma
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Wen-Bin Zheng
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Quan Zhao
  • College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Xing-Quan Zhu
  • Corresponding author
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
  • College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-03-30 | DOI: https://doi.org/10.1515/ap-2016-0050

Abstract

Enterocytozoon bieneusi is one of the most important zoonotic pathogen that can infect almost all animals, including humans. However, little information is available regarding prevalence and genotypes of E. bieneusi in sika deer. In the present study, the prevalence of E. bieneusi infection in sika deer in Jilin province, Northeastern China was examined using PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. 23 (7.06%) of 326 samples were tested E. bieneusi-positive, and the risk factor significantly associated with E. bieneusi prevalence was the age of sika deer. Sequence analysis of the ITS rRNA gene suggested that 8 genotypes of E. bieneusi were found in this study, with five known genotypes, namely J (n = 11), BEB6 (n = 4), EbpC (n = 1), CHN-DC1 (n = 1), KIN-1 (n = 1) and three novel genotypes, namely JLD-1 (n = 2), JLD-2 (n = 2) and JLD-3 (n = 1). Phylogenetic analysis indicated that genotypes CHN-DC-1, KIN-1, EbpC, JLD-2 and JLD-3 fell into group 1, while other three genotypes (genotypes J, BEB6 and JLD-1) were clustered into group 2 (so-called bovine-specific groups). These findings indicated the presence of zoonotic E. bieneusi in Jilin province, Northeastern China. Effective strategies should be performed to control E. bieneusi infection in sika deer, other animals and humans.

Keywords: Enterocytozoon bieneusi; sika deer; prevalence; genotypes; Northeastern China

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About the article


Received: 2015-09-23

Revised: 2015-10-09

Accepted: 2015-12-30

Published Online: 2016-03-30

Published in Print: 2016-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0050.

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© W. StefańskiInstitute of Parasitology, PAS. Copyright Clearance Center

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