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Acta Parasitologica

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1896-1851
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Volume 61, Issue 2 (Jun 2016)

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First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan

Makoto Matsubayashi
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
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/ Hideko Takayama
  • Kumamoto Prefectural Chuo Livestock Hygiene Service Center, Kumamoto, Kumamoto 861–4215, Japan
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/ Masahiro Kusumoto
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
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/ Misato Murata
  • Kumamoto Prefectural Chuo Livestock Hygiene Service Center, Kumamoto, Kumamoto 861–4215, Japan
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/ Yuka Uchiyama / Masaya Kaji
  • Kumamoto Prefectural Johoku Livestock Hygiene Service Center, Yamaga, Kumamoto 861-0304, Japan
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/ Kazumi Sasai
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
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/ Ryosaku Yamaguchi
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
  • JA Zen-noh (National Federation of Agricultural Co-operative Associations) Institute of Animal Health, Ohja-machi, Sakura, Chiba 285-0043, Japan
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/ Tomoyuki Shibahara
  • Corresponding author
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
  • Email
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Published Online: 2016-03-30 | DOI: https://doi.org/10.1515/ap-2016-0054

Abstract

Cystoisospora suis is a pathogen that causes diarrhea in pigs and can lead to serious disease. Species identification, especially by histopathological examination, is often difficult because of morphologically similar parasites such as Eimeria species. In this study, we used histopathological, bacteriological, virological, and parasitological methods to identify the cause of the disease in two piglets with severe diarrhea. Villous atrophy, diffuse necrosis, and flattening of mucosal epithelial cells were found in the ilea of examined piglets, and coccidian parasites were found in the cytoplasm of the epithelial cells. In some merozoites in the meronts, the presence of two nuclei indicated type 1 merozoites, characteristic of C. suis. According to Cystoisospora-specific PCR targeting the rRNA internal transcribed spacer 1 (ITS1) gene, the sequences of the products were 98.5% similar to those of C. suis. Escherichia coli (O149 serogroup) exhibiting a virulence factor profile (LT, STb, and EAST1 as toxins and F4 as a colonization factor) was detected in one piglet. No other bacteria or significant enteric viruses were found. Co-infection with C. suis and E. coli could imply aggravation of the disease, although further study is needed to assess the pathogenicity of this interaction. This study is the first to clarify by molecular analysis the sequences of C. suis detected in piglets in Japan.

Keywords: Cystoisospora suis; Escherichia coli; ITS1 gene; piglet

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About the article

Received: 2015-10-22

Revised: 2015-11-19

Accepted: 2016-01-14

Published Online: 2016-03-30

Published in Print: 2016-06-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0054.

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