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Acta Parasitologica

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1896-1851
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Volume 61, Issue 3 (Sep 2016)

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In vitro observation of the stage conversion of transgenic Toxoplasma gondii RH strain expressing dual fluorescent proteins

Qijun Song
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Ximeng Sun
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Yongsheng Ji
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Xinlei Yan
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Jun Zou
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Shiyun Zhao
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Xun Suo
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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/ Xingquan Zhu
  • State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
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/ Xianyong Liu
  • Corresponding author
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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Published Online: 2016-07-21 | DOI: https://doi.org/10.1515/ap-2016-0061

Abstract

Toxoplasma gondii converts from tachyzoites to bradyzoites after acute infection and thus survives the attack of the host immune responses. In this study, we observed the conversion of tachyzoites to bradyzoites in cell cultures using a transgenic T. gondii RH strain. The transgenic parasites continuously express yellow fluorescent protein (YFP) but only express red fluorescent protein (RFP) at the bradyzoite stage. Red fluorescent bradyzoite-containing cysts were found in transgenic parasite infected cells cultured with atmospheric CO2 supply, indicating the successful induction of the stage conversion. In cell culture with alkalic medium (pH 8.1) and atmospheric CO2 supply, only part of the YFP-expressing parasites in a cyst express RFP marker, suggesting the asynchronous development of T. gondii in vitro. This study provides a possibility for further studies of the gene expression profile during stage conversion and the genes involved.

Keywords: Toxoplasma gondii; transgenic; fluorescent proteins; stage conversion; asynchronous development

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About the article

Received: 2015-11-03

Revised: 2016-01-01

Accepted: 2016-02-04

Published Online: 2016-07-21

Published in Print: 2016-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0061.

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