Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

4 Issues per year

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

See all formats and pricing
More options …
Volume 61, Issue 3


Prevalence and first molecular identification of Sarcocystis species in cattle and water buffaloes in India

Monal Daptardar
  • School of Public Health & Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Balbir Bagicha Singh
  • Corresponding author
  • School of Public Health & Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rabinder Singh Aulakh
  • School of Public Health & Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jatinder Paul Singh Gill
  • School of Public Health & Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-07-21 | DOI: https://doi.org/10.1515/ap-2016-0069


The importance of Sarcocystis hominis in causing zoonotic infections is well known. Recently, S. hominis like cysts have been reported from water buffalo in China. Previous studies indicate prevalence of Sarcocystis species in bovine populations in India but molecular evidence is required for proper species differentiation. We examined two hundred and ninety six cardiac tissue samples of Indian water buffaloes and cattle from northern and western parts of the country. Tissues were examined for Sarcocystis using intact cyst isolation method, pepsin acid digestion method and Sarcocystis 18S rRNA PCR. The combination of primers was used for 18S rRNA PCR amplification followed by sequencing. Twenty five representative samples were sent for sequencing and 19 readable sequences were obtained for phylogenetic analysis. Overall, the Sarcocystis cysts/zoites were recorded in 44% (95% CI 38–49%), 58% (95% CI 53–64%) and 68% (95% CI 63–73%) from both cattle and buffalo samples using intact cyst isolation, pepsin-HCl digestion method and conventional PCR, respectively. The results indicate that pepsin-HCl digestion method and conventional PCR are more sensitive than intact cyst isolation for detection of Sarcocystis species in tissue samples. The prevalence of Sarcocystis species was high in buffalo as compared to cattle intermediate hosts. Phylogenetic analysis indicated that more than one Sarcocystis species are circulating in cattle and water buffaloes in India. The results further indicate that experimental transmission studies are required to re-confirm the identities and host ranges of the Sarcocystis species in cattle and water buffaloes in India.

Keywords: Cattle; India; molecular identification; prevalence; Sarcocystis species; water buffaloes


  • Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410Google Scholar

  • Domenis L., Peletto S., Sacchi L., Clementi E., Genchi M., Felisari L., Felisari C., Modesto P., Zuccon F., Campanella C., Maurella C., Guidetti C., Acutis P.L. 2011. Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitology Research, 109, 1677–87. DOI: CrossrefGoogle Scholar

  • Dubey J.P. 1982a. Development of ox-coyote cycle of Sarcocystis cruzi. The Journal of Protozoology, 29, 591–601Google Scholar

  • Dubey J.P. 1982b. Development of the ox-cat cycle of Sarcocystis hirsuta. Proceedings of the Helminthological Society of Washington, 49, 295–04Google Scholar

  • Dubey J.P., Fayer R., Rosenthal B.M., Calero-Bernal R., Uggla A. 2014. Identity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle (Bos taurus) and the suppression of Sarcocystis sinensis as a nomen nudum. Veterinary Parasitology, 205, 1–6 DOI: CrossrefGoogle Scholar

  • Dubey J.P., Lindsay D.S. 2006. Neosporosis, Toxoplasmosis, and Sarcocystosis in Ruminants. Veterinary Clinics of North America: Food Animal Practice, 22, 645–71Google Scholar

  • Dubey J.P., Udtujan R.M., Cannon L., Lindsay O.S. 1990. Condemnation of beef because of Sarcocystis hirsuta infection. Journal of American Veterinary Medical Association, 196, 1095-96.Google Scholar

  • Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791Google Scholar

  • Gajadhar A.A., Lalonde L.F., Al-Adhami B., Singh B.B., Lobanov V. 2015. Chapter 6 Foodborne apicomplexan protozoa: Coccidia: In: Gajadhar AA (ed) Foodborne parasites in food supply web- occurrence and control (Eds.). Published by WoodHead Publishing, London, pp. 101–147Google Scholar

  • Hilali M., El-Seify M., Zayed A., El-Morsey A., Dubey J.P. 2011. Sarcocystis dubeyi (Huong and Uggla 1999) infection in water buffaloes (Bubalus bubalis) from Egypt. The Journal of Parasitology, 3, 527–28Web of ScienceGoogle Scholar

  • Imes G.D., Migaki G. 1967. Eosinophilic myositis in cattle-pathology and incidence. Proceedings of the 71st Annual Meeting of the US Livestock Sanitary Association. 111–112Google Scholar

  • Jacobs L., Remington J., Melton M. 1960. A survey of meat samples from swine, cattle and sheep for the presence of encysted Toxoplasma. The Journal of Parasitology, 46, 23–28Google Scholar

  • Juyal P.D., Ruprah N., Chhabra M. 1989. Rapid isolation of intact micro-sarcocystis Protozoan-Apicomplexa cysts from muscular tissues. Indian Journal of Animal Health, 22, 69–71Google Scholar

  • Latif B., Delemi J., Mohammed B., Al-Bayati S., Al-Amiry A. 1999. Prevalence of Sarcocystis spp. in meat- producing animals in Iraq. Veterinary Parasitology, 84, 85–89Google Scholar

  • Luna L.G. (Ed.). 1968. Manual of Histological Staining Methods. Armed Forces Institute of Pathology, McGraw Hill Book Company, pp. 34–157Google Scholar

  • Mehlhorn H., Hartley W.J., Heydorn A.O. 1976. A comparative ultrastructure study of the cyst wall of 13 Sarcocystis species. Protistologica 12, 451–67Google Scholar

  • Mohanty B.N., Misra S.C., Panda D.N., Panda M.R. 1995. Prevalence of Sarcocystis infection in ruminants in Orissa. Indian Veterinary Journal, 72, 1026–30Google Scholar

  • Newcombe R.G. 1998. Two-Sided Confidence Intervals for the Single Proportion: Comparison of Seven Methods. Statistics in Medicine, 17, 857–872Google Scholar

  • Olsen O.W. 1974. Animal Parasite: Their lifecycle and ecology. Courier dove publications, USA 3, 1–562Google Scholar

  • Rosenthal B. 2010. Sarcocystis. In. Liu D (Ed.). Molecular detection of food borne pathogens. CRC press, Boca Raton, USA, pp. 735–740Google Scholar

  • Saitou N., Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425Google Scholar

  • Singh B.B., Sharma R., Sharma J.K., Juyal P.D. 2010. Parasitic Zoonoses in India: An overview. Revue Scientifique et Technique, Office International des Epizootics, 29, 629–637Google Scholar

  • Tamura K., Nei M., Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences USA, 101, 11030–11035Google Scholar

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729Google Scholar

  • Venu R., Hafeez M.D. 2000. Prevalence of Sarcocystis infections in slaughtered domestic ruminants in Tirupati (A.P). Indian Veterinary Journal, 77, 165–66Google Scholar

  • Wilson E. B. 1927. Probable Inference, the Law of Succession, and Statistical Inference. Journal of the American Statistical Association, 22, 209–212Google Scholar

  • Yang Z.Q., Zuo Y.X., Ding B. 2001. Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. The Journal of Parasitology, 87, 934–37Google Scholar

About the article

Received: 2015-08-10

Revised: 2016-01-25

Accepted: 2016-03-08

Published Online: 2016-07-21

Published in Print: 2016-09-01

Citation Information: Acta Parasitologica, Volume 61, Issue 3, Pages 523–528, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0069.

Export Citation

© W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in