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Acta Parasitologica

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Volume 61, Issue 3 (Sep 2016)

Issues

Vaccination with a cocktail of Ancylostoma ceylanicum recombinant antigens leads to worm burden reduction in hamsters

Marcin Wiśniewski
  • Corresponding author
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Maciej Łapiński
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Emilia Daniłowicz-Luebert
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
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  • De Gruyter OnlineGoogle Scholar
/ Sławomir Jaros
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ewa Długosz
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
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  • De Gruyter OnlineGoogle Scholar
/ Halina Wędrychowicz
  • Division of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
  • Department of Molecular Biology, Laboratory of Molecular Parasitology, W. Stefański Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda St., 00-818, Warsaw, Poland
  • Other articles by this author:
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Published Online: 2016-07-21 | DOI: https://doi.org/10.1515/ap-2016-0074

Abstract

Hookworms, a group to which Ancylostoma ceylanicum belongs, are gastrointestinal nematodes that infect more than 700 million people around the world. They are a leading cause of anemia in developing countries. In order to effectively prevent hookworm infections research is conducted to develop an effective vaccine using recombinant antigens of the parasite. The aim of this study was to examine the influence of the hosts’ on protection against ancylostomiasis and the shaping of the humoral immune response among Syrian hamsters after immunization with a cocktail of five A. ceylanicum recombinant antigens. Ace-ASP-3, Ace-ASP-4, Ace-APR-1, Ace-MEP-6 and Ace-MEP-7 were obtained in the pET expression system. Immunization with a vaccine cocktail resulted in a 33.5% worm burden reduction. The immunogenicity of the recombinant proteins were determined using ELISA. Statistical analysis showed that vaccinated hamsters developed stronger humoral responses to four of five recombinant antigens (the exception being Ace-ASP-3) compared to hamsters from the control group.

Keywords: Hookworm; Ancylostoma ceylanicum; recombinant antigen; vaccine

References

  • Albonico M., Bickle Q., Ramsan M., Montresor A., Savioli L., Taylor M. 2003. Efficacy of mebendazole and levamisole alone or in combination against intestinal nematode infections after repeated targeted mebendazole treatment in Zanzibar. Bulletin of the World Health Organization, 81, 343–352Google Scholar

  • Albonico M., Smith P.G., Ercole E., Hall A., Chwaya H.M., Alawi K.S., Savioli L. 1995. Rate of reinfection with intestinal nematodes after treatment of children with mebendazole or albendazole in a highly endemic area. Transactions of the Royal Society of Tropical Medicine and Hygiene, 89, 538–341Google Scholar

  • Bąska P., Wisniewski M., Krzyzowska M., Długosz E., Zygner W., Górski P., Wędrychowicz H. 2013. Molecular cloning and characterization of in vitro immune response against astacin-like metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum. Experimental Parasitology, 133, 472–482. DOI: CrossrefGoogle Scholar

  • Bethony J., Loukas A., Smout M., Brooker S., Mendez S., Plieskatt J., Goud G., Bottazzi M.E., Zhan B., Wang Y., Williamson A., Lustigman S., Correa-Oliveira R., Xiao S., Hotez P.J. 2005. Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. FASEB Journal, 19, 1743–1745. DOI: CrossrefGoogle Scholar

  • Bungiro R.D., Harrison L.M., Cappello M. 2002. Ancylostoma ceylanicum Excretory/Secretory Protein 1: Purification and molecular cloning of a major secretory protein from adult hookworms. Molecular and Biochemical Parasitology, 119, 147–151. DOI:CrossrefGoogle Scholar

  • Bungiro R.D. Jr, Solis C.V., Harrison L.M., Cappello M. 2004. Purification and molecular cloning of and immunization with Ancylostoma ceylanicum excretory-secretory protein 2, an immunoreactive protein produced by adult hookworms. Infection and Immunity, 72, 2203–2213. DOI: CrossrefGoogle Scholar

  • Cantacessi C., Campbell B.E., Visser A., Geldhof P., Nolan M., Nisbet A.J., Matthews J.B., Loukas A., Hofmann A., Otranto D., Sternberg P.W., Gasser R.B. 2009. A portrait of the ‘SCP/TAPS’ proteins of eukaryotes – Developing a framework for fundamental research and biotechnological outcomes. Biotechnology Advances, 27, 376–388. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Chu D., Bungiro R.D., Ibanez M., Harrison L.M., Campodonico E., Jones B.F., Mieszczanek J., Kuzmic P., Cappello M. 2004. Molecular characterization of Ancylostoma ceylanicum Kunitz-type serine protease inhibitor: evidence for a role in hookworm-associated growth delay. Infection and Immunity, 72, 2214–2221. DOI: CrossrefGoogle Scholar

  • Długosz E., Wisniewski M., Wójcik L., Wędrychowicz H. 2010. In vitro IL-4, IL-12, and IFN-γ production by splenocytes from Ancylostoma ceylanicum infected hamsters. Bulletin of the Veterinary Institute in Pulawy, 54, 321–325Google Scholar

  • Fairfax K.C., Vermeire J.J., Harrison L.M., Bungiro R.D., Grant W., Husain S.Z., Cappello M. 2009. Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum. International Journal for Parasitology, 39, 1561–1571. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Goud G.N., Zhan B., Ghosh K., Loukas A., Hawdon J., Dobardzic A., Deumic V., Liu S., Dobardzic R., Zook B.C., Jin Q., Liu Y., Hoffman L., Chung-Debose S., Patel R., Mendez S., Hotez P.J. 2004. Cloning, yeast expression, isolation, and vaccine testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma ceylanicum. The Journal of Infectious Diseases, 189, 919–929. DOI: CrossrefGoogle Scholar

  • Hotez P.J., Ashcom J., Zhan B., Bethony J., Loukas A., Hawdon J., Wang Y., Jin Q., Jones K.C., Dobardzic A., Dobardzic R., Bolden J., Essiet I., Brandt W., Russell P.K., Zook B.C., Howard B., Chacon M. 2003. Effect of vaccination with a recombinant fusion protein encoding an astacinlike metalloprotease (MTP-1) secreted by host-stimulated Ancylostoma caninum third-stage infective larvae. Journal of Parasitology, 89, 853–855. DOI:CrossrefGoogle Scholar

  • Hotez P.J., Diemert D., Bacon K.M., Beaumier C., Bethony J.M., Bottazzi M.E., Brooker S., Couto A.R., da Silva Freire M., Homma A., Lee B.Y., Loukas A., Loblack M, Morel C.M., Oliveira R. C., Russell P.K. 2013. The human hookworm vaccine. Vaccine, 31, 227–232. DOI: CrossrefGoogle Scholar

  • Hotez P.J., Fenwick A., Savioli L., Molyneux D.H. 2009. Rescuing the bottom billion through control of neglected tropical diseases. Lancet, 373, 1570–1575. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Loukas A., Bethony J.M., Mendez S., Fujiwara R.T., Goud G.N., Ranjit N., Zhan B., Jones K., Bottazzi M.E., Hotez P.J. 2005. Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine, 2, 1008–1017. DOI: CrossrefGoogle Scholar

  • Mendez S., Zhan B., Goud G., Ghosh K., Dobardzic A., Wu W., Liu S., Deumic V., Dobardzic R., Liu Y., Bethony J., Hotez P.J. 2005. Effect of combining the larval antigens Ancylostoma secreted protein 2 (ASP-2) and metalloprotease 1 (MTP-1) in protecting hamsters against hookworm infection and disease caused by Ancylostoma ceylanicum. Vaccine, 23, 3123–3130. DOI: CrossrefGoogle Scholar

  • Palmer D.R., Bradley M., Bundy D.A. 1996. IgG4 responses to antigens of adult Necator americanus: potential for use in large-scale epidemiological studies. Bulletin of the World Health Organization, 74, 381–386Google Scholar

  • Pearson M.S., Bethony J.M., Pickering D.A., de Oliveira L.M., Jariwala A., Santiago H., Miles A.P., Zhan B., Jiang D., Ranjit N., Mulvenna J., Tribolet L., Plieskatt J., Smith T., Bottazzi M.E., Jones K., Keegan B., Hotez P.J., Loukas A. 2009. An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection. FASEB Journal, 23, 3007–3019. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Pearson M.S., Jariwala A.R., Abbenante G., Plieskatt J., Wilson D., Bottazzi M.E., Hotez P.J., Keegan B., Bethony J.M., Loukas A. 2015. New tools for NTD vaccines: A case study of quality control assays for product development of the human hookworm vaccine Na-APR-1M74. Human Vaccines and Immunotherapeutics, 11, 1251–1257. DOI: CrossrefGoogle Scholar

  • de Silva N.R. 2003. Impact of mass chemotherapy on the morbidity due to soil-transmitted nematodes. Acta Tropica, 86, 197–214. DOI: CrossrefGoogle Scholar

  • Siwińska A.M., Bąska P., Daniłowicz-Luebert E., Januszkiewicz K., Długosz E., Wędrychowicz H., Cappello M., Wisniewski M. 2013. Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins. Acta Parasitologica, 58, 112–118. DOI: CrossrefGoogle Scholar

  • Stoltzfus R.J., Dreyfuss M.L., Chwaya H.M., Albonico M. 1997. Hookworm control as a strategy to prevent iron deficiency. Nutrition Reviews, 55, 223–232. DOI: CrossrefGoogle Scholar

  • Xiao S., Zhan B., Xue J., Goud G.N., Loukas A., Liu Y., Williamson A., Liu S., Deumic V., Hotez P. 2008. The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) challenged with human hookworm, Necator americanus. Experimental Parasitology, 118, 32–40. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Wasyl K., Zawistowska-Deniziak A., Bąska P., Wędrychowicz H., Wisniewski M. 2013. Molecular cloning and expression of the cDNA sequence encoding a novel aspartic protease from Uncinaria stenocephala. Experimental Parasitology, 134, 220–227. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Wędrychowicz H. 2015. Antiparasitic DNA vaccines in 21st century. 2015. Acta Parasitologica, 60, 179–189. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Wiśniewski M., Jaros S., Bąska P., Cappello M., Długosz E., Wędrychowicz H. 2016. Molecular cloning and analysis of Acemep-7 cDNA vaccine efficiency on the laboratory model Ancylostoma ceylanicum – Syrian hamster. Experimental Parasitology, 163, 1–7. DOI: CrossrefGoogle Scholar

  • Wiśniewski M., Jaros S., Bąska P., Cappello M., Wędrychowicz H. 2013. Ancylostoma ceylanicum metalloproteases 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica, 58, 367–383. DOI: CrossrefWeb of ScienceGoogle Scholar

  • Wiśniewski M., Lapiński M., Zdziarska A.M., Długosz E., Bąska P. 2014. Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase. Molecular and Biochemical Parasitology, 196, 12–20. DOI: CrossrefGoogle Scholar

  • Zhan B., Liu Y., Badamchian M., Williamson A., Feng J., Loukas A., Hawdon J.M., Hotez P.J. 2003. Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum. International Journal for Parasitology, 33, 897–907. DOI: CrossrefGoogle Scholar

About the article

Received: 2016-01-04

Revised: 2015-03-16

Accepted: 2016-03-23

Published Online: 2016-07-21

Published in Print: 2016-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0074.

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