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Acta Parasitologica

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1896-1851
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Volume 61, Issue 3 (Sep 2016)

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Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain

María Reyes-Batlle
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Carolina Wagner
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
  • Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
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/ Jonadab Zamora-Herrera
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Alejandro Vargas-Mesa
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Ines Sifaoui
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
  • Laboratoire Matériaux-Molécules et Applications, IPEST, University of Carthage, La Marsa, Tunisia
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/ Ana C. González
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Atteneri López-Arencibia
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Basilio Valladares
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Enrique Martínez-Carretero
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ José E. Piñero
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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/ Jacob Lorenzo-Morales
  • Corresponding author
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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Published Online: 2016-07-21 | DOI: https://doi.org/10.1515/ap-2016-0088

Abstract

In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of V. vermiformis strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of Vermamoeba. After a week, V. vermiformis amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of Vermamoeba vermiformis in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised.

Keywords: Vermamoeba vermiformis; water; Lanzarote; Canary Islands; Spain

References

  • Adamska M., Leonska-Duniec A., Lanocha N., Skotarczak B. 2014. Thermophilic pathogenic amoebae isolated from natural water bodies in Poland and their molecular characterization. Acta Parasitologica, 59, 433–441Google Scholar

  • Bonilla-Lemus P., Caballero Villegas A.S., Carmona Jiménez J., Lugo Vázquez A. 2014. Occurrence of free-living amoebae in streams of the Mexico Basin. Experimental Parasitology, 145 Suppl, S28–33Web of ScienceGoogle Scholar

  • Fouque E., Trouilhé M.C., Thomas V., Humeau P., Héchard Y. 2014. Encystment of Vermamoeba (Hartmannella) vermiformis: Effects of environmental conditions and cell concentration. Experimental Parasitology, 145 Suppl, S62–68Web of ScienceGoogle Scholar

  • Fouque E., Yefimova M., Trouilhé M.C., Quellard N., Fernandez B., Rodier M.H., Thomas V., Humeau P., Héchard Y. 2015. Morphological Study of the Encystment and Excystment of Vermamoeba vermiformis Revealed Original Traits. Journal of Eukaryotic Microbiology, 62, 327–337Google Scholar

  • Lasjerdi Z., Niyyati M., Lorenzo-Morales J., Haghighi A., Taghipour N. 2015. Ophthalmology hospital wards contamination to pathogenic free living Amoebae in Iran. Acta Parasitologica, 60, 417–422Web of ScienceGoogle Scholar

  • Lorenzo-Morales J., Khan N.A., Walochnik J. 2015. An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22, 10Google Scholar

  • Lorenzo-Morales J., Monteverde-Miranda C.A., Jiménez C., Tejedor M.L., Valladares B., Ortega-Rivas A. 2005b. Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain. Annals of Agricultural and Environmental Medicine, 12, 233–236Google Scholar

  • Lorenzo-Morales J., Ortega-Rivas A., Foronda P., Martínez E., Valladares B. 2005a. Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitology Research, 95, 273–277Google Scholar

  • Page E.C. 1988. A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, Cumbria, England, UKGoogle Scholar

  • Pagnier I., Valles C., Raoult D., La Scola B. 2015. Isolation of Vermamoeba vermiformis and associated bacteria in hospital water. Microbial Pathogenesis, 80, 14–20Google Scholar

  • Reyes-Batlle M., Niyyati M., Martín-Navarro C.M., López-Arencibia A., Valladares B., Martínez-Carretero E., Piñero J.E., Lorenzo-Morales J. 2015. Unusual Vermamoeba vermiformis strain isolated from Snow in Mount Teide, Tenerife, Canary Islands, Spain. Novelty in Biomedicine, 3, 189–192Google Scholar

  • Reyes-Batlle M., Todd C.D., Martín-Navarro C.M., López-Arencibia A., Cabello-Vílchez A.M., González A.C., Córdoba-Lanús E., Lindo J.F., Valladares B., Piñero J.E., Lorenzo-Morales J. 2014. Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain. Parasitology Research, 113, 1383–1388Web of ScienceGoogle Scholar

  • Richards A.M., Von Dwingelo J.E., Price C.T., Abu Kwaik Y. 2013. Cellular microbiology and molecular ecology of Legionella-amoeba interaction. Virulence, 4, 307–314Google Scholar

  • Smirnov A.V., Chao E., Nassonova E.S., Cavalier-Smith T. 2011. A revised classification of naked lobose amoebae (Amoebozoa: lobosa). Protist, 162, 545–570Web of ScienceGoogle Scholar

  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739Google Scholar

  • Thomas V., Loret J.F., Jousset M., Greub G. 2008. Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant. Environmental Microbiology, 10, 2728–2745Google Scholar

  • Thomas V., McDonnell G., Denyer S.P., Maillard J.Y. 2010. Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality. FEMS Microbiology Reviews, 34, 231–259Web of ScienceGoogle Scholar

About the article

Received: 2015-02-16

Revised: 2016-03-14

Accepted: 2016-03-17

Published Online: 2016-07-21

Published in Print: 2016-09-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0088.

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© W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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