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Acta Parasitologica

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Volume 61, Issue 4 (Dec 2016)

Issues

First report of mixed Entamoeba polecki (ST 1) and E. suis infection in piglets shedding abnormalfeces by histopathological and molecular surveys

Makoto Matsubayashi
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
  • Other articles by this author:
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/ Yuri Sasagawa
  • Chuou Animal Health and Hygiene Service Center, Niigata Prefecture, Nishikan-ku, Niigata 959-0423, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tsunehiko Aita
  • Chuou Animal Health and Hygiene Service Center, Niigata Prefecture, Nishikan-ku, Niigata 959-0423, Japan
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  • De Gruyter OnlineGoogle Scholar
/ Masaharu Tokoro
  • Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Takara-machi, Kanazawa 920-8640, Japan
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/ Makoto Haritani
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
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/ Tomoyuki Shibahara
  • Corresponding author
  • National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305–0856, Japan
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-10-22 | DOI: https://doi.org/10.1515/ap-2016-0093

Abstract

Of the three species of Entamoeba found in swine, namely E. histolytica, E. polecki and E. suis, E. histolytica can also infect humans, producing colitis or abscesses of liver and leading to death. However, the pathogenicity of other species of Entamoeba has not been fully characterized. Here, we conducted histopathology and molecular surveys on a pig farm where piglets had blackish feces or muddy diarrhea. Histopathological examination of two piglets showed necrosis of the mucous surface at the ileum, cecum, or colon, infiltration of neutrophils, and formation of ulcers. Based on morphological characteristics, E. polecki and E. suis trophozoites were mainly detected at lamina propria and surface of the lesion, respectively, and Lawsonia intracellularis, a bacterial pathogen, was also detected. Molecular analysis using the small subunit ribosomal RNA gene on other piglets and a sow revealed infection with both E. polecki and E. suis. These findings corroborate our previous reports that the two Entamoeba spp. are pathogenic in pigs as aggravations of symptoms with L. intracellularis. This is the first report about mixed infection with E. polecki and E. suis.

Keywords: Entamoeba suis; Entamoeba polecki; mixed infection; pigs

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About the article

Received: 2015-12-09

Revised: 2016-05-06

Accepted: 2016-05-16

Published Online: 2016-10-22

Published in Print: 2016-12-01


Conflict of interest

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0093.

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© 2016 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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