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Acta Parasitologica

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Volume 61, Issue 4 (Dec 2016)

Issues

Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil

Jonatas Campos de Almeida
  • Laboratory of Zoonoses and Public Health, Universidade Estadual de Londrina, PR, 86057-970, Brazil
  • Other articles by this author:
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/ Michelle Salmon Frehse
  • Laboratory of Zoonoses and Public Health, Universidade Estadual de Londrina, PR, 86057-970, Brazil
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  • De Gruyter OnlineGoogle Scholar
/ Italmar Teodorico Navarro
  • Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, PR, 86057-970, Brazil
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  • De Gruyter OnlineGoogle Scholar
/ João Luis Garcia
  • Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, PR, 86057-970, Brazil
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  • De Gruyter OnlineGoogle Scholar
/ Alexander Welker Biondo / Roberta Lemos Freire
  • Corresponding author
  • Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, PR, 86057-970, Brazil
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Published Online: 2016-10-22 | DOI: https://doi.org/10.1515/ap-2016-0097

Abstract

The aim of the present study was to determine the prevalence of antibodies to Toxoplasma gondii by two serological techniques in sera of 364 stray dogs from Brazil by immunofluorescence antibody test (IFAT, cut off point 1:16) and to the modified agglutination test (MAT, cut-off points 1:25 and 1:50). A total of 175/364 (48.07%) sera were positive by IFAT, and 108/364 (29.67%) and 85/364 (23.35%) were positive by MAT with cutoff points 1:25 and 1:50, respectively were positive by MAT. Cohen’s Kappa Coefficient between IFAT and MAT was 0.81 (excellent) and 0.66 (substantial) with cutoff points 1:25 and 1:50, respectively. Using IFAT as gold standard, MAT sensitivity and specificity were 78% and 99% for 1:25 and 61% and 99% for 1:50, respectively. The results document of the usefulness of MAT for serological diagnosis because it does not require species-specific conjugate.

Keywords: Toxoplasma gondii; stray dog; MAT; IFAT; sentinel

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About the article

Received: 2016-03-11

Revised: 2016-06-08

Accepted: 2016-06-14

Published Online: 2016-10-22

Published in Print: 2016-12-01


Conflict of interest statement

The authors declare no conflicts of interest.


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0097.

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