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Acta Parasitologica

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1896-1851
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Volume 61, Issue 4 (Dec 2016)

Issues

Fasciola hepatica infection in humans: overcoming problems for the diagnosis

Silvana Carnevale
  • Instituto Nacional de Enfermedades Infecciosas – ANLIS “Dr. Carlos G. Malbrán”. Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
  • Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jorge Bruno Malandrini
  • Facultad de Ciencias de la Salud. Universidad Nacional de Catamarca. Maestro Quiroga 1ra. Cuadra, (CP 4700) San Fernando del Valle de Catamarca, Catamarca, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María Laura Pantano
  • Instituto Nacional de Enfermedades Infecciosas – ANLIS “Dr. Carlos G. Malbrán”. Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mirna Sawicki
  • Hospital Municipal de Infecciosas “Dr. Francisco Javier Muñiz”. Uspallata 2272, (CP 1282) Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Claudia Cecilia Soria
  • Facultad de Ciencias de la Salud. Universidad Nacional de Catamarca. Maestro Quiroga 1ra. Cuadra, (CP 4700) San Fernando del Valle de Catamarca, Catamarca, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lein Hung Kuo / Laura Kamenetzky
  • Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
  • Instituto de Microbiología y Parasitología Médica (IMPaM), UBA-CONICET. Paraguay 2155 piso 13, (CP 1121), Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Osvaldo Germán Astudillo
  • Instituto Nacional de Enfermedades Infecciosas – ANLIS “Dr. Carlos G. Malbrán”. Avenida Vélez Sarsfield 563, (CP 1281) Ciudad de Buenos Aires, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jorge Néstor Velásquez
  • Corresponding author
  • Hospital Municipal de Infecciosas “Dr. Francisco Javier Muñiz”. Uspallata 2272, (CP 1282) Ciudad de Buenos Aires, Argentina
  • Email
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Published Online: 2016-10-24 | DOI: https://doi.org/10.1515/ap-2016-0107

Abstract

Fascioliasis is a zoonosis actually considered as a foodborne trematode disease priority by the World Health Organization. Our study presents three cases of F. hepatica infection diagnosed by direct, indirect and/or imaging diagnostic techniques, showing the need of the combined use of them. In order to overcome some difficulties of the presently available methods we show for the first time the application of molecular tools to improve human fascioliasis diagnosis by employing a PCR protocol based on a repetitive element as target sequence. In conclusion, diagnosis of human fascioliasis has to be carried out by the combination of diagnostic techniques that allow the detection of infection in different disease phases, different epidemiological situations and known/new transmission patterns in the actual scenario.

Keywords: Fascioliasis; polymerase chain reaction; enzyme-linked immunosorbent assay

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About the article

Received: 2015-11-13

Revised: 2016-05-13

Accepted: 2016-06-27

Published Online: 2016-10-24

Published in Print: 2016-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0107.

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© 2016 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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