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Acta Parasitologica

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Volume 61, Issue 4 (Dec 2016)

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Genotyping of clinical isolates of Acanthamoeba genus in Venezuela

Carolina Wagner
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María Reyes-Batlle
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María Alejandra Vethencourt Ysea
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
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  • De Gruyter OnlineGoogle Scholar
/ Mónica V. Galindo Pérez
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
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  • De Gruyter OnlineGoogle Scholar
/ Carmen Guzmán de Rondón
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anaibeth J. Nessi Paduani
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
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  • De Gruyter OnlineGoogle Scholar
/ Angelyseb Dorta Pérez
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Atteneri López-Arencibia
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
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  • De Gruyter OnlineGoogle Scholar
/ Ines Sifaoui
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
  • Laboratoire Matériaux-Molécules et Applications, IPEST, University of Carthage, La Marsa, Tunisia
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  • De Gruyter OnlineGoogle Scholar
/ María Virginia Pérez de Galindo
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Eva Pérez de Suárez
  • Laboratorio de Amibiasis-Cátedra de Parasitología, Escuela de Bioanálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
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/ Enrique Martínez-Carretero
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
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/ Basilio Valladares
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
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/ José E. Piñero
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
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/ Jacob Lorenzo-Morales
  • Corresponding author
  • University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain
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Published Online: 2016-10-24 | DOI: https://doi.org/10.1515/ap-2016-0110

Abstract

Free-living amoebae of Acanthamoeba genus are opportunistic pathogens distributed worldwide. Strains included in this genus are causative agents of a fatal encephalitis and a sight-threating keratitis in humans and other animals. In this study, 550 clinical samples which were collected between 1984 and 2014 from different patients with suspected infections due to Acanthamoeba were initially screened for the presence of this amoebic genus at the Laboratorio de Amibiasis-Escuela de Bioanálisis at the Universidad Central de Venezuela. Samples were cultured in 2% Non-Nutrient agar plates seeded with a layer of heat killed Escherichia coli. From the 550 clinical samples included in this study, 18 of them were positive for Acanthamoeba genus after culture identification. Moreover, positive samples were confirmed after amplification of the Diagnostic Fragment 3 (DF3) of the Acanthamoeba18S rDNA genus and sequencing was carried out in order to genotype the isolated strains of Acanthamoeba. Furthermore, the pathogenic potential of the strains was checked by performing thermotolerance and osmotolerance assays. Sequencing of the DF3 region resulted in the identification of genotype T4 in all the isolated strains. Moreover, most isolates were thermotolerant or both thermotolerant and osmotolerant and thus were classified as potentially pathogenic strains. To the best of our knowledge, this is the first report on the molecular characterization at the genotype level of Acanthamoeba strains in Venezuela.

Keywords: Acanthamoeba spp; Venezuela; clinical samples; genotype

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About the article

Received: 2016-06-15

Revised: 2016-06-29

Accepted: 2016-06-30

Published Online: 2016-10-24

Published in Print: 2016-12-01


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0110.

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