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Acta Parasitologica

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Volume 61, Issue 4


The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland)

Patrycja Opalińska
  • Corresponding author
  • Department of Game Management and Forest Protection, Poznañ University of Life Sciences, Wojska Polskiego 71d 60-625 Poznañ, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna Wierzbicka
  • Department of Game Management and Forest Protection, Poznañ University of Life Sciences, Wojska Polskiego 71d 60-625 Poznañ, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marek Asman
  • Department of Parasitology, Medical University of Silesia, Jedności 8, 41-218 Sosnowiec, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-10-24 | DOI: https://doi.org/10.1515/ap-2016-0117


The study was performed in the Trzciel Forest Districts which is located in the west part of Poland. The Scots pine is the main tree species creating forest landscape there. Dermacentor reticulatus, usually found in wet, boggy and damp habitat, in this case was discovered in fresh mixed coniferous forest and fresh coniferous forest. In Central Europe the Dermacentor reticulatus is after Ixodes ricinus the second most important vector for tick-borne diseases in Europe. The ticks were collected by flagging from lower vegetation during the autumn peak of their activity. All Dermacentor reticulatus were checked for presence of tickborne pathogens using PCR and nested PCR. In total 125 Dermacentor reticulatus ticks were collected. Among the pathogens examined, only Babesia microti has been found in 4% of them whereas Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum have not been found. In this research the presence of Babesia. microti in Dermacentor reticulatus has been proved. It should be stressed that it is a new focus for the Dermacentor reticulatus tick in Western Poland.

Keywords: Dermacentor reticulatus; Babesia microti; Borrelia burgdorferi sensu lato; Anaplasma phagocytophilum; Trzciel Forest District; new focus of the occurrence


  • Asman M., Solarz K., Cuber P., Gąsior T., Szilman P., Szilman E., Tondas E., Matzullok A., Kusion N., Florek K. 2015. Detection of protozoans Babesia microti and Toxoplasma gondii and their co-existence in ticks (Acari: Ixodida) collected in Tarnogórski district (Upper Silesia, Poland). Annals of Agricultural and Environmental Medicine, 22, 80–83. CrossrefGoogle Scholar

  • Blaňarová L., Stanco M., Miklisová D., Víchová B., Mošanský L., Kralijik J., Bona M., Derdáková M. 2016. Presence of Condidatus Neoehrlichia mikurensis and Babesia microti in rodents and two tick species (Ixodes ricinus and Ixodes trianguliceps) in Slovakia. Ticks and Tick-borne Diseases, 7, 319–326Google Scholar

  • Bullová E., Lukáň M., Stanko M., Pet'ko B. 2009. Spatial distribution of Dermacentor reticulatus tick in Slovakia in the beginning of the 21st century. Veterinary Parasitology, 1, 357–360Google Scholar

  • Cardoso L., Yisaschar-Mekuzas Y., Rodrigues F.T., Costa Á., Machado J., Diz-Lopes D., Baneth G. 2010. Research Canine babesiosis in northern Portugal and molecular characterization of vector-borne co-infections. Parasites & Vectors, 3, 27Google Scholar

  • Chitimia-Dobler L. 2015. Spatial distribution of Dermacentor reticulatus in Romania. Veterinary Parasitology, 214, 219–223Google Scholar

  • Cochez C., Lempereur L., Madder M., Claerebout E., Simons L., De Wilde N., Linden A., Saegerman C., Heyman P., Losson B. 2012. Foci report on indigenous Dermacentor reticulatus populations in Belgium and preliminary study of associated babesiosis pathogens. Medical and Veterinary Entomology, 26, 355–358Google Scholar

  • Cżyzowski P., Drozd L., Karpiński M. 2008. The species of wild game in Lublin. Urban wildlife. Protect the biodiversity in cities. (Eds. P. Indykiewicz, L. Jerzak, T. Barczak). SAR “Pomorze”, Bydgoszcz: 537–540 (In Polish)Google Scholar

  • Domínguez-Peñafiel G., Giménez-Pardo C., Gegúndez M.I., Lledó L. 2011. Prevalence of ectoparasite arthropods on wild animals and cattle in the Las Merindades area (Burgos, Spain). Parasite, 18, 251–260Google Scholar

  • Estrada-Peña A., Bouattour A., Camicas J.L., Walker A.R. 2004. Ticks of Domestic Animals in the Mediterranean Region. International Consortium on Ticks and Tickborne Diseases-2. Atlanta pp.59-61Google Scholar

  • Forest management plan for Trzciel Forest District for 2006 – 2015. Biuro Urzqdzania Lasu i Geodezji Lesnej oddzial w Gorzowie Wielkopolskim. (In Polish)Google Scholar

  • Gilbert L., Jones L.D., Hudson P.J., Gould E.A., Reid H.W. 2000. Role of small mammals in the persistence of louping-ill virus: field survey and tick co-feeding studies. Medical and Veterinary Entomology, 14: 277–282. CrossrefGoogle Scholar

  • Gray J. S., Dautel H., Estrada-Peña A., Kahl O., Lindgren E. 2009. Effects of Climate Change on Ticks and Tick-Borne Diseases in Europe. Interdisciplinary Perspectives on Infectious Diseases, Article ID 593232. CrossrefGoogle Scholar

  • Gray J., Zintel A., Hildebrandt A., Hunfeld K.P., Weiss L. 2010. Zoonotic babesiosis: overview of the disease and novel aspects of pathogen identity. Ticks and Tick-Borne Diseases, 1, 3–10Google Scholar

  • Holler J.G., Roser D., Nielsen H.V., Eickhardt S., Chen M., Lester A., Bang D., Frandsen C., David K.P. 2013. A case of human babesiosis in Denmark. Travel Medicine and Infectious Disease, 11, 324–328Google Scholar

  • Karbowiak G. (Ed.) 2009. The meadow ornate tick Dermacentor reticulatus – the occurrence, biology and role as a vector of tick-borne diseases, Agencja Reklamowo-Wydawnicza Arkadiusz Grzegorczyk, Warszawa (In Polish)Google Scholar

  • Karbowiak G. 2014. The occurrence of the Dermacentor reticulatus tick – its expasion to new areas and possible causes. Annals of Parasitology, 60, 37–47Google Scholar

  • Karbowiak G., Kiewra D. 2010. New locations of Dermacentor reticulatus ticks in Western Poland: the first evidence of merge in D. reticulatus occurence areas. Annals of Parasitology, 56, 333–336Google Scholar

  • Kiewra D., Czulowska A. 2013. Evidence for an increased distribution range of Dermacentor reticulatus in south-west Poland. Experimental and Applied Acarology Journal, 59, 501–506Google Scholar

  • Kiffner C., Lödige C., Alings M., Vor T., Rühe F. 2010. Abudance estimation of Ixodes ricinus (Acari: Ixodidae) on roe deer (Capreolus capreolus). Experimental and Applied Acarology Journal, 52, 73–84Google Scholar

  • Mierzejewska E.J., Pawełczyk A., Radkowski M., Welc-Falçciak R., Bajer A. 2015. Pathogens vectored by the tick, Dermacentor reticulatus, in endemic regions and zones of expansion in Poland. Parasites and Vectors, 8, 490. CrossrefGoogle Scholar

  • Mierzejewska E. J., Estrada-Peña A., Alsarraf M., Kowalec M. 2016. Mapping of Dermacentor reticulatus expansion in Poland in 2012–2014. Ticks and Tick-borne Diseases, 7, 94–106. DOI :10.1016/j.ttbdis.2015.09.003CrossrefGoogle Scholar

  • Nosek J. 1972. The ecology and public health importance of Dermacentor marginatus and D.reticulatus ticks in Central Europe. Folia Parasitologica 19, 93–102Google Scholar

  • Nowak M. 2011. Discovery of Dermacentor reticulatus (Fabricius, 1794) (Acari: Amblyommidae) standings in the Lubuskie Province (Western Poland). Experimental and Applied Acarology, 54, 191–197Google Scholar

  • Nowak-Chmura M. 2013. The ticks fauna (Ixodida) of Central Europe. Wydawnictwo Naukowe Uniwersytetu Pedagogicznego, Kraków (In Polish)Google Scholar

  • Overzier E., Pfister K., Herbb I., Mahling M., Böck Jr. G., Silaghi C. 2013. Detection of tick-borne pathogens in roe deer (Capreolus capreolus), in questing ticks (Ixodes ricinus), and in ticks infesting roe deer in southern Germany. Ticks and Tick-borne Diseases, 4, 320–328 CrossrefGoogle Scholar

  • Paulauskas A., Radzijevska J., Mardosantė-Busaitienė D., Aleksan-dravičiene A., Galdikas M., Krikštolaitis R. 2015. New localities of Dermacentor reticulatus ticks in the Baltic countries. Ticks and Tick-borne Diseases, 6, 5, 630–635. CrossrefGoogle Scholar

  • Persing D.H., Mathiensen D., Marshal W.F., Telford S.R., Spielman A., Thomford J.W., Conrad P.A. 1992. Detection of Babesia microti by polymerase chain reaction. Journal of Clinical Microbiology, 30, 2097–2103Google Scholar

  • Piesman J., Eisen L. 2008. Prevention of tick-bome diseases. Annual Review of Entomology, 53, 323–343Google Scholar

  • Reye A.L., Stegniy V., Mishaeva N.P., Velhin S., Hübschen J.M., Ig-natyev G., Muller C.P. 2013. Prevalence of Tick-Borne Pathogens in Ixodes ricinus and Dermacentor reticulatus Ticks from Different Geographical Locations in Belarus. PLoS ONE 8. CrossrefGoogle Scholar

  • Rubel F., Brugger K., Pfeffer M., Chitimia-Dobler L., Didyk Y.M., Leverenz S., Dautel H., Kahl O. 2016. Geographical distribution of Dermacentor marginatus and Dermacentor reticulatus in Europe. Ticks and Tick-borne Diseases. 7, 224–233. CrossrefGoogle Scholar

  • Ruiz-Fons F., Fernández-de-Mera I.G., Acevedo P., Höfle U., Vicente J., de la Fuente J., Gortázar C. 2006. Ticks parasitizing Iberian red deer (Cervus elaphus hispanicus) and European wild boar (Sus scrofa) from Spain: geographical and temporal distribution. Veterinary Parasitology, 140, 133–142. CrossrefGoogle Scholar

  • Ruiz-Fons F., Gilbert L. 2010. The role of deer as vehicles to move ticks, Ixodes ricinus, between contrasting habitats. International Journal for Parasitology, 40, 1013–1020. CrossrefGoogle Scholar

  • Schreiber C., Krücken J., Beck S., Maaz D., Pachnicke S., Krieger K., Gross M., Kohn B., von Samson-Himmelstjerna G. 2014. Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany. Parasites & Vectors, 7, 535. CrossrefGoogle Scholar

  • Siuda K. (Ed.)1993. Ticks of Poland (Acari: Ixodida). Part II. Systematic and distribution. The Polish Parasitological Society, Warszawa (In Polish)Google Scholar

  • Smith F. D., Ballantyne R., Morgen E. R., Wall R. 2011. Prevalence, distribution and risk associated with tick infestation of dogs in Great Britain. Medical and Veterinary Entomology, 25, 377384. CrossrefGoogle Scholar

  • Starńzak J., Gabre R.M., Krumnis-Lozowska W., Racewicz M., Kubica-Biernat B. 2004. Ixodes ricinus as a vector of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in urban and suburban forests. Annals of Agricultural and Environmental Medicine, 11, 109–114Google Scholar

  • Stańczak J., Racewicz M., Kubicka-Biernat B., Kruminis-Łozowska W., Dąbrowski J., Adamczyk A., Markowska M. 1999. Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinu sticks (Acari, Ixodidae) in different Polish woodlands. Annals of Agricultural and Environmental Medicine, 6, 127–132Google Scholar

  • Szymański S. 1986. Distribution of the Dermacentor reticulatus (Fabricius 1794) (Ixodidae) in Poland. Acta Parasitologica, 31, 143–154Google Scholar

  • Tijsse-Klasen E., Hansford K.M., Jahfari S., Phipps P., Sprong H., Medlock J.M. 2013. Spotted fever group rickettsiae in Dermacentor reticulatus and Haemaphysalis punctata ticks in the UK. Parasites & Vectors, 6, 212Google Scholar

  • Wirtgen M., Nahayo A., Linden A., Losson B., Garigliany M., Desmecht D. 2011. Detection of Anaplasma phagocytophilum in Dermacentor reticulatus ticks. Veterinary Record, 168, 195Google Scholar

  • Wodecka B., Skotarczak B. 2000. Genetic variability of Borrelia burgdorferi s.l. in ticks Ixodes ricinus collected in north-west-ern Poland. Annals of Parasitology, 46, 475–485Google Scholar

  • Wójcik-Fatla A., Bartosik K., Buczek A., Dutkiewicz J. 2012. Babesia microti in Adult Dermacentor reticulatus Ticks from estern Poland. Vector Borne Zoonotic Diseases, 12, 841–843Google Scholar

  • Wójcik-Fatla A., Cisak E., Zając V., Zwoliński J., Dutkiewicz J. 2011. Prevelence of tick-borne encephalitis virus in Ixodes ricinus and Dermacentor reticulatus ticks collected from Lublin region (eastern Poland). Ticks and Tick-Borne Diseases, 2, 16–19Google Scholar

  • Wójcik-Fatla A., Szymańska J., Wdowiak L., Buczek A., Dutkiewicz J. 2009. Coincidence of three pathogens (Borrrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia mi-croti) in Ixodes ricinus ticks in the Lublin Macroregion. Annals of Agricultural and Environmental Medicine, 16, 151158Google Scholar

  • Zygner W., Górski P., Wędrychowicz H. 2009. New localities of Dermacentor reticulatus tick (vector of Babesia canis canis) in central and eastern Poland. Polish Journal of Veterinary Sciences, 12: 549–555Google Scholar

About the article

Received: 2016-03-08

Revised: 2016-07-26

Accepted: 2016-08-05

Published Online: 2016-10-24

Published in Print: 2016-12-01

Citation Information: Acta Parasitologica, Volume 61, Issue 4, Pages 849–854, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2016-0117.

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