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Acta Parasitologica

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Volume 62, Issue 1


Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients

Sonali Khanduja
  • Corresponding author
  • Departments of Microbiology, Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India
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/ Ujjala Ghoshal
  • Departments of Microbiology, Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India
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  • De Gruyter OnlineGoogle Scholar
/ Uday C. Ghoshal
  • Departments of Microbiology, Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India
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Published Online: 2016-12-28 | DOI: https://doi.org/10.1515/ap-2017-0007


Enterocytozoon bieneusi (E. bieneusi), infecting renal transplant (RT) recipients may be transmitted anthroponotically or zoonotically. Accordingly, we aimed to, a) evaluate genotypes of E. bieneusi ínfecting RT recipients, and b) infer phylogenetic interpretation on transmission of different genotypes among infected hosts. Stool samples of 22 RT recipients infected with microsporidia (identified using modified trichrome staining) were subjected to species identification. All E. bieneusi positive samples were subjected to genotyping. The phylogenetic tree was constructed using Mega 5 software. Of 22 microsporidia infected RT recipients, 21 (95.5%) had E. bieneusi. ITS sequences of 21 E. bieneusi were classified into eight genotypes (Ind1 to Ind8). Among them, 4 (Ind5 to Ind8) were novel. Genotypes Ind2, Ind3, Ind4, Ind7 and Ind8 showed close sequence similarity to genotypes reported exclusively from humans. Phylogenetic analysis further supported their anthroponotic transmission. Genotypes Ind1, Ind5 and Ind6 showed close sequence similarity to genotypes reported from both animals and humans. Phylogenetic analysis further supported their zoonotic transmission. Anthroponotic transmission of E. bieneusi was more common among males (11, 100% vs. 7/10, 70%; P = 0.05), presenting with diarrhea (11, 100% vs. 6/10, 60%; P = 0.02) and watery stool (10/11, 91% vs. 5/10, 50%; P = 0.03).

Keywords: Microsporidia; intestinal microsporidiosis; immune-compromised patients; internal transcribed spacer; genotyping


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About the article

Received: 2016-04-01

Revised: 2016-08-03

Accepted: 2016-09-28

Published Online: 2016-12-28

Published in Print: 2017-03-01

Citation Information: Acta Parasitologica, Volume 62, Issue 1, Pages 63–68, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0007.

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