Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica


IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2018: 1.00

SCImago Journal Rank (SJR) 2018: 0.500
Source Normalized Impact per Paper (SNIP) 2018: 0.664

More options …
Volume 62, Issue 1

Issues

Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey

Esin Guven
  • Corresponding author
  • Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hamza Avcioglu
  • Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ahmet Deniz
  • Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, Ankara, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ İbrahim Balkaya
  • Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ugur Abay / Şevki Yavuz / Muzaffer Akyüz
Published Online: 2016-12-28 | DOI: https://doi.org/10.1515/ap-2017-0025

Abstract

Equine piroplasmosis (EP) is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by Theileria equi and Babesia caballi. There are studies reporting the presence of equine piroplasmosis in Turkey but the situation in Erzurum is unknown. The aim of the current study was to determine the situation of equine piroplasmosis in jeered horses in Erzurum. Between April and August 2015, a total of 125 Arabian horse were examined and blood samples were collected. At the time of sampling, animals were also examined for tick infestations and clinical signs. Besides microscopic examination of Giemsastained blood smears, multiplex PCR performed with species specific primers partially amplifying the 18S rRNA gene of B. caballi and T. equi. During the microscopic examination of blood smears, T. equi piroplasms were found in 6 (4.8%) samples. In total, 11 (8.8%) T. equi DNA were detected with multiplex PCR. B. caballi piroplasms or DNA were not obtained. BLAST analysis of the sequenced T. equi samples (GenBank: KU921661-KU921667) indicated 98.8–100% identity to each other, and 100% similarity to T. equi isolates in South Africa, Iran, China, Sudan, India, Mongolia, Trinidad, Kenya, Spain, Serbia, Bosnia and Herzegovina and Turkey (Bursa). The results of our study indicate that T. equi occurs more frequently than B. caballi in the study area. To the authors’ knowledge, this is the first report of the molecular detection of equine piroplasmosis in jeered horses in Erzurum, Turkey.

Keywords: Equine piroplasmosis; horse; jereed; Turkey

References

  • Abedi V., Razmi G., Seifi H., Naghibi A. 2014. Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran. Ticks and Tick-borne Diseases, 5, 239–244. CrossrefGoogle Scholar

  • Acici M., Umur S., Guvenc T., Arslan H.H., Kurt M. 2008. Seroprevalence of Equine babesiosis in the Black Sea region of Turkey. Parasitology Internatinal, 57, 198–200. CrossrefGoogle Scholar

  • Alhassan A., Pumidonming W., Okamura M., Hirata H., Battsetseg B., Fujisaki K., et al. 2005. Development of a single round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood. Veterinary Parasitology, 129, 43–49. CrossrefGoogle Scholar

  • Allsopp M.T., Allsopp B.A. 2006. Molecular sequence evidence for the reclassification of some Babesia species. Annals of the New York Academy of Sciences, 1081, 509–517. CrossrefGoogle Scholar

  • Asgarali Z., Coombs D.K., Mohammed F., Campbell M.D., Caesar E. 2007. A serological study of Babesia caballi and Theileria equi in thoroughbreds in Trinidad. Veterinary Parasitology, 144, 167–171. CrossrefGoogle Scholar

  • Aydin L., Bakirci S. 2007. Geographical distribution of ticks in Turkey. Parasitology Research, 101, 163–166. CrossrefGoogle Scholar

  • Bartolomé Del Pino L.E., Roberto N., Vincenzo V., Francesca I., Antonella C., Luca A.G., et al. 2016. Babesia caballi and Theileria equi infections in horses in Central-Southern Italy: Sero-molecular survey and associated risk factors. Ticks and Tick-borne Diseases, 7, 462–469. CrossrefGoogle Scholar

  • Bashiruddin J.B., Cammá C., Rebêlo E. 1999. Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16s rRNA gene. Veterinary Parasitology, 84, 75–83Google Scholar

  • Bhagwan J., Kumar A., Kumar R., Goyal L., Goel P., Kumar S. 2015. Molecular evidence of Theileria equi infection in Hyalomma anatolicum ticks infested on sero-positive Indian horses. Acta Parasitologica, 60, 322–329. CrossrefGoogle Scholar

  • Bhoora R., Quan M., Matjila P.T., Zweygarth E., Guthrie A.J., Collins N.E. 2010. Sequence heterogeneity in the equi merozoite anti-gen gene (ema-1) of Theileria equi and development of an ema-1-specific TaqMan MGBTM assay for the detection of T. equi. Veterinary Parasitology, 172, 33–45. CrossrefGoogle Scholar

  • Brüning A. 1996. Equine piroplasmosis an update on diagnosis, treatment and prevention. British Veterinary Journal, 152, 139–151Google Scholar

  • Chauvin A., Moreau E., Bonnet S., Plantard O., Malandrin L. 2009. Babesia and its hosts: adaptation to long-lasting interactions as a way to achieve efficient transmission. Veterinary Research, 40, 37. CrossrefGoogle Scholar

  • Davitkov D., Vucicevic M., Stevanovic J., Krstic V., Slijepcevic D., Glavinic U., Stanimirovic Z. 2016. Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan. Acta Parasitologica, 61, 337–342. CrossrefGoogle Scholar

  • Deniz A., Karaer Z., Çakmak A., Pişkin Ç., Balkaya İ., Vatansever Z., Nalbantoğlu S. 2007. Türkiye’de atlarda Babesia etkenlerinin saptanmasinda PCR tekniğinin uygulanmasi (TAGEM 2007, Project number: HS/01/02/06/05/103)Google Scholar

  • De Waal T. 1992. Equine piroplasmosis: A review. British Veterinary Journal, 148, 6–14. CrossrefGoogle Scholar

  • Duzlu O. 2013. Atlarin vektör kaynakli protozoon hastaliklari. 17th National Parasitology Congress Proceedings, Denizli, Turkey, pp. 51–54Google Scholar

  • Farkas R., Tánczos B., Gyurkovszky M., Földvári G., Solymosi N., Edelhofer R., Hornok S. 2013. Serological and molecular detection of Theileria equi infection in horses in Hungary. Veterinary Parasitology, 192, 143–148. CrossrefGoogle Scholar

  • Gallusová M., Qablan M.A., D’Amico G., Oborník M., Petrzelková K.J., Mihalca A.D, Modry’ D. 2014. Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir. Veterinary Parasitology, 206, 287–292. CrossrefGoogle Scholar

  • Garcia-Bocanegra I., Arenas-Montes A., Hernández E., Adaszek L., Carbonero A., Almeria S., et al. 2013. Seroprevalence and risk factors associated with Babesia caballi and Theileria equi infection in equids. Veterinary Journal, 195, 172–178. CrossrefGoogle Scholar

  • Guidi E., Pradier S., Lebert I., Leblond A. 2015. Piroplasmosis in an endemic area: analysis of the risk factors and their implications in the control of Theileriosis and Babesiosis in horses. Parasitology Researche, 114, 71–83. CrossrefGoogle Scholar

  • Güçjü H.Z., Karaer K.Z. 2007. Ankara Yöiesinde Sportif ve Gösteri Amaçli Yetiştirilen Atlarda Babesia caballi (Nuttall, 1910) ve Theileria equi (Syn. Babesia equi, Laveran, 1901)’nin Yayilişmm Polimeraz Zincir Reaksiyonu ile Araştmlmasi. Türkiye Parazitoloji Dergisi, 31, 89–93. (In Turkish)Google Scholar

  • Hailat N.Q., Lafi S.Q., Al-Darraji A.M., Al-Ani F.K. 1997. Equine babesiosis associated with strenuous exercise: clinical and pathological studies in Jordan. Veterinary Parasitology, 69, 1–8. CrossrefGoogle Scholar

  • Heim A., Passos L.M.F., Ribeiro M.F.B., Costa-Junior L.M., Bastos C.V., CabralD.D., et al. 2007. Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil. Parasitology Research, 102, 63–68. CrossrefGoogle Scholar

  • Hussain M.H., Saqib M., Fahad Raza Muhammad G., Asi M.N., Mansoor M.K., Saleem M., Jabbar A. 2014. Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab, Pakistan. Veterinary Parasitology, 202, 248–256. CrossrefGoogle Scholar

  • Jaffer O., Abdishakur F., Hakimuddin F., Riya A., Wernery U., Schuster R.K., 2010. A comparative study of serological tests and PCR for the diagnosis of equine piroplasmosis. Parasitology Research, 106, 709–713. CrossrefGoogle Scholar

  • Kizilarslan F., Yildirim A., Duzlu O., Inci A., Onder Z., Ciloglu A. 2015. Molecular Detection and Characterization of Theileria equi and Babesia caballi in Horses (Equus ferus caballus) in Turkey. Journal of Equine Veterinary Science, 35, 830–835. CrossrefGoogle Scholar

  • Krause P.K. 2003. Babesiosis Diagnosis and Treatment. Vector Borne and Zoonotic Diseases, 3, 45–51. CrossrefGoogle Scholar

  • Mahmoud M.S., Abu El-Ezz N.T., Abdel-Shafy S., Nassar S.A., El Namaky A.H., Khalil W.K.B., et al. 2016. Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches. Parasites and Vectors, 9, 260. CrossrefGoogle Scholar

  • Mehlhorn H., Schein E. 1998. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998. Parasitology Research, 84, 467–475Google Scholar

  • Moretti A., Mangili V., Salvatori R., Maresca C., Scoccia E., Torina A., et al. 2010. Prevalence and diagnosis of Babesia and Theileria infections in horses in Italy: A preliminary study. Veterinary Journal, 184, 346–350. CrossrefGoogle Scholar

  • Mujicaa F.F., Perrone T., Forlano M., Coronado A., Meléndez R.D., Barrios N., et al. 2011. Serological prevalence of Babesia caballi and Theileria equi in horses of Lara State, Venezuela. Veterinary Parasitology, 178, 180–183. CrossrefGoogle Scholar

  • Munkhjargal T., Sivakumar T., Battsetseg B., Nyamjargal T., Aboulaila M., Purevtseren B., et al. 2013. Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. Infection, Genetics and Evolution, 16, 178–185. CrossrefGoogle Scholar

  • OIE Terrestrial Manual 2014. Equine Piroplasmosis. NB: Version adopted by the World Assembly of Delegates of the OIE in May 2014 CHAPTER 2.5.8Google Scholar

  • Qablan M.A., Oborník M., Petrželková K.J., Sloboda M., Shudiefat M.F., Hořín P., et al. 2013. Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity. Parasitology, 140, 1096–1103. CrossrefGoogle Scholar

  • Rothschild C.M. 2013. Equine piroplasmosis. Journal of Equine Veterinary Science, 33, 497–508. CrossrefGoogle Scholar

  • Schnittger L., Rodriguez A.E., Florin-Christensen M., Morrison, D.A. 2012. Babesia: A world emerging. Infection, Genetics and Evolution, 12, 1788–1809. CrossrefGoogle Scholar

  • Sevinc F., Maden M., Kumas C., Sevinc M., Ekici O.D. 2008. A comparative study on the prevalence of Theileria equi and Babesia caballi infections in horse sub-populations in Turkey. Veterinary Parasitology, 156, 173–177. CrossrefGoogle Scholar

  • Sgorbini M., Bonelli F., Nardoni S., Rocchigiani G., Corazza M., Mancianti F. 2015. Seroprevalence and Molecular Analysis of Babesia caballi and Theileria equi in Horses from Central Italy During a 10-Year Period Journal of Equine Veterinary Science, 35, 865–868. CrossrefGoogle Scholar

  • Sigg L., Gerber V., Gottstein B., Doherr M.G., Frey C.F. 2010. Seroprevalence of Babesia caballi and Theileria equi in the Swiss horse population. Parasitology International, 59, 313–317. CrossrefGoogle Scholar

  • Slivinska K., Víchová B., Werszkoc J., Szewczyk T., Wróblewski Z., Petko B., et al. 2016. Molecular surveillance of Theileria equi and Anaplasma phagocytophilum infections in horses from Ukraine, Poland and Slovakia. Veterinary Parasitology, 215, 35–37. CrossrefGoogle Scholar

  • Sloboda M., Jirkú M., Lukesova D., Qablan M.A., Batsukh Z., Fiala I., et al. 2011. A survey of piroplasmids in horses and Bactrian camels in North-Eastern Mongolia. Veterinary Parasitology,179, 246–249. CrossrefGoogle Scholar

  • Yilmaz O., Ertugrul M., Boztepe S. 2012. Some Morphological Traits of Arabian Horses Used for Javelin Swarm (Cirit) Game in Erzurum. Journal of Animal Science Advances, 2, 659–663Google Scholar

  • Yilmaz O., Wilson R.T. 2013. The Domestic Livestock Resources of Turkey: Occurrence and Control of Diseases of Horses, Donkeys and Mules. Journal of Equine Veterinary Science, 33, 1021–1030. CrossrefGoogle Scholar

  • Takeet M., Adeleye A., Adebayo O.O., Akande F. 2009. Haematology and serum biochemical alteration in stress induced equine theileriosis. A case report. The Scientific World Journal, 4, 19–21Google Scholar

  • Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24, 1596–1599. CrossrefGoogle Scholar

  • Tamzali Y. 2013. Equine piroplasmosis: An updated review. Equine Veterinary Education, 25, 590–598. CrossrefGoogle Scholar

  • Wise L.N., Kappmeyer L.S., Mealey R.H., Knowles D.P. 2013. Review of Equine Piroplasmosis. Journal of Veterinary Internal Medicine, 27, 1334–1346. CrossrefGoogle Scholar

About the article

Received: 2016-08-28

Revised: 2016-11-08

Accepted: 2016-11-17

Published Online: 2016-12-28

Published in Print: 2017-03-01


Conflict of interest statement:None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.


Citation Information: Acta Parasitologica, Volume 62, Issue 1, Pages 207–213, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0025.

Export Citation

© 2017 W. Stefański Institute of Parasitology, PAS.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sharon Tirosh-Levy, Yuval Gottlieb, Ori Arieli, Monica L. Mazuz, Roni King, Igal Horowitz, and Amir Steinman
Ticks and Tick-borne Diseases, 2019, Page 101286
[2]
Handi Dahmana, Nadia Amanzougaghene, Bernard Davoust, Thomas Normand, Olivier Carette, Jean-Paul Demoncheaux, Baptiste Mulot, Bernard Fabrizy, Pierre Scandola, Makhlouf Chik, Florence Fenollar, and Oleg Mediannikov
Veterinary Parasitology: Regional Studies and Reports, 2019, Volume 18, Page 100332
[3]
Punsantsogvoo Myagmarsuren, Thillaiampalam Sivakumar, Batsaikhan Enkhtaivan, Batdorj Davaasuren, Myagmar Zoljargal, Sandagdorj Narantsatsral, Batbold Davkharbayar, Bayasgalan Mungun-Ochir, Banzragch Battur, Noboru Inoue, Ikuo Igarashi, Badgar Battsetseg, and Naoaki Yokoyama
Journal of Parasitology, 2019, Volume 105, Number 4, Page 580
[4]
ThankGod E. Onyiche, Keisuke Suganuma, Ikuo Igarashi, Naoaki Yokoyama, Xuenan Xuan, and Oriel Thekisoe
International Journal of Environmental Research and Public Health, 2019, Volume 16, Number 10, Page 1736
[5]
Mehmet Ozkan Timurkan and Hakan Aydın
Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 2019, Volume 14, Number 1, Page 71
[6]
Sezayi Ozubek and Munir Aktas
Comparative Immunology, Microbiology and Infectious Diseases, 2018
[7]
Fabio Bernardo Schein, Maerle Oliveira Maia, Rute Witter, Arlei Marcili, Lázaro Manoel de Camargo, Valéria Dutra, Luciano Nakazato, Stefhano Luís Candido, Elianara Martins de Almeida, Anderson Castro Soares de Oliveira, and Richard de Campos Pacheco
Revista Brasileira de Parasitologia Veterinária, 2018
[8]
Djursun Karasartova, Ayse Semra Gureser, Tuncay Gokce, Bekir Celebi, Derya Yapar, Adem Keskin, Selim Celik, Yasemin Ece, Ali Kemal Erenler, Selma Usluca, Kosta Y. Mumcuoglu, Aysegul Taylan-Ozkan, and Nicholas P. Day
PLOS Neglected Tropical Diseases, 2018, Volume 12, Number 4, Page e0006395
[9]
Maria Guadalupe Montes Cortés, José Luis Fernández-García, and Miguel Ángel Habela Martínez-Estéllez
Parasite, 2017, Volume 24, Page 14

Comments (0)

Please log in or register to comment.
Log in