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Acta Parasitologica


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Volume 62, Issue 1

Issues

Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves

Yuu Matsuura
  • Toubu Veterinary Clinic, Chiba Prefectural Federation of Agricultural Mutual Aid Association, Sanmu, Chiba 289-1326, Japan
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
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/ Makoto Matsubayashi
  • Corresponding author
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
  • National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan
  • Email
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/ Satoko Nukata
  • Toubu Veterinary Clinic, Chiba Prefectural Federation of Agricultural Mutual Aid Association, Sanmu, Chiba 289-1326, Japan
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/ Tomoyuki Shibahara / Osamu Ayukawa
  • Toubu Veterinary Clinic, Chiba Prefectural Federation of Agricultural Mutual Aid Association, Sanmu, Chiba 289-1326, Japan
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/ Yasuko Kondo
  • Toubu Veterinary Clinic, Chiba Prefectural Federation of Agricultural Mutual Aid Association, Sanmu, Chiba 289-1326, Japan
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/ Tomohide Matsuo
  • Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan
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/ Shigehiko Uni
  • Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
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/ Masaru Furuya
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
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/ Hiroyuki Tani
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
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/ Naotoshi Tsuji
  • Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan
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/ Kazumi Sasai
  • Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan
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Published Online: 2016-12-28 | DOI: https://doi.org/10.1515/ap-2017-0026

Abstract

In the production and management of beef and dairy cattle, controlling diarrhea is one of the important concerns. Pathogenic agents of the disease, protozoan parasites including Cryptosporidium spp., are difficult to control, making prevention, diagnoses, and treatment of diarrhea. In the present study, we investigated a farm with a history of calf deaths over a period of 10 years in order to determine the cause of disease and to clarify the detailed distribution of the pathogens. In four examined calves that were reared in calf pens, all were positive with Cryptosporidium and/or Giardia, while the other breeding stock and adult cattle were negative. Molecular analyses revealed that the isolates from calves were C. parvum subtype IIaA15G2R1 as a zoonotic and G. intestinalis assemblage E. Other pathogenic bacteria and diarrhea-causing viruses were not detected. After treating the calf pens with boiling water and milk of lime (Ca[OH]2), oocysts of C. parvum and cysts of G. intestinalis were not found and no additional calves died. This is the first report to describe the mixed infection of both parasites in Japan.

Keywords: Cryptosporidium parvum; genotyping; Giardia intestinalis; neonatal calves

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About the article

Received: 2016-10-21

Revised: 2016-11-23

Accepted: 2016-11-24

Published Online: 2016-12-28

Published in Print: 2017-03-01


Citation Information: Acta Parasitologica, Volume 62, Issue 1, Pages 214–220, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0026.

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