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Acta Parasitologica

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1896-1851
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Volume 62, Issue 2 (Jun 2017)

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PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks

Tian-Yin Cheng
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China
  • Hunan Co-Innovation Center of Animal Production Safety, Changsha, Hunan Province 410128, PR China
/ Guo-Hua Liu
  • Corresponding author
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China
  • Hunan Co-Innovation Center of Animal Production Safety, Changsha, Hunan Province 410128, PR China
  • Email:
Published Online: 2017-04-18 | DOI: https://doi.org/10.1515/ap-2017-0034

Abstract

Ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with Haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Eleven distinct DGGE bands were found using PCR-DGGE method. Sequences analyses indicated that they belonged to Bacillus cereus, Candidatus rickettsia, Erwinia sp., Klebsiella pneumoniae, Pectobacterium carotovorum, Pseudomonas aeruginosa, Rickettsia peacockii, Rickettsia helvetica, Rickettsia slovaca, Staphylococcus simulans and Uncultured bacterium clone. Our find that the K. pneumoniae and P. aeruginosa isolates were presented in all H. flava ticks in different developmental stages. The present results indicated that zoonotic pathogens are present in H. flava ticks in Henan province, China. To our knowledge, this is the first report on intestinal bacterial flora associated with H. flava ticks in China.

Keywords: Haemaphysalis flava; Intestinal microbiota; DGGE; China

References

  • Dantas-Torres F., Chomel B.B., Otranto D. 2012. Ticks and tickborne diseases: a one health perspective. Trends Parasitology, 28, 437–446. [Crossref]

  • Diuk-Wasser M.A., Vannier E., Krause P.J. 2016. Coinfection by Ixodes Tick-Borne Pathogens: Ecological, Epidemiological, and Clinical Consequences. Trends Parasitology, 32, 30–42. [Crossref]

  • Fang L.Q., Liu K., Li X.L., Liang S., Yang Y., Yao H.W., et al. 2015. Emerging tick-borne infections in mainland China: an increasing public health threat. Lancet Infectious Diseases, 15, 1467–1479. [Crossref]

  • Gao X., Nasci R., Liang G. 2010. The neglected arboviral infections in mainland China. PLoS Neglect Tropical Diseases, 4, e624. [Crossref]

  • Karbowiak G., Biernat B., Werszko J., Rychlik L. 2016. The transstadial persistence of tick-borne encephalitis virus in Dermacentor reticulatus ticks in natural conditions. Acta Parasitologica, 61, 201–203. [Crossref]

  • Kušar D., Avguštin G. 2012. Optimization of the DGGE band identification method. Folia Microbiology, 57, 301–306. [Crossref]

  • Liu L., Li L., Liu J., Hu Y., Liu Z., Guo L., Liu J. 2013. Coinfection of Dermacentor silvarum olenev (acari: ixodidae) by Coxiella-Like, Arsenophonus-like, and Rickettsia-like symbionts. Applied and Environment Microbiology, 79, 2450–2454. [Crossref]

  • Liu Q., He B., Huang S.Y., Wei F., Zhu X.Q. 2014. Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis. Lancet Infectious Diseases, 14, 763–772. [Crossref]

  • Moreno C.X., Moy F., Daniels T.J., Godfrey H.P., Cabello F.C. 2006. Molecular analysis of microbial communities identified in different developmental stages of Ixodes scapularis ticks from Westchester and Dutchess Counties, New York. Environment Microbiology, 8, 761–772

  • Opalińska P., Wierzbicka A., Asman M. 2016. The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland). Acta Parasitologica, 61, 849–854. [Crossref]

  • Schabereiter G.C., Lubitz W., Rölleke S. 2003. Application of broad-range 16S rRNA PCR amplification and DGGE fingerprinting for detection of tick-infecting bacteria. Journal of Microbiology Methods, 52, 251–260

  • Sun J., Lin J., Gong Z., Chang Y., Ye X., Gu S., et al.2015. Detection of spotted fever group Rickettsiae in ticks from Zhejiang Province, China. Experimental and Applied Acarology, 65, 403–411. [Crossref]

  • Tveten A.K., Riborg A., Vadseth H.T. 2013. DGGE identification of microorganisms associated with Borrelia burgdorferi Sensu Lato-or Anaplasma phagocytophilum-infected Ixodes ricinus ticks from northwest Norway. International Journal of Microbiology, 2013, 805456. [Crossref]

  • Tveten A.K., Sjåstad K.K. 2011. Identification of bacteria infecting Ixodes ricinus ticks by 16S rDNA amplification and denaturing gradient gel electrophoresis. Vector-Borne and Zoonotic Diseases, 11, 1329–1334. [Crossref]

  • Van Overbeek L., Gassner F., Van Der Plas C.L., Kastelein P., Nunesda Rocha U., Takken W. 2008. Diversity of Ixodes ricinus tick-associated bacterial communities from different forests. FEMS Microbiology Ecology, 66, 72–84. [Crossref]

  • Wu X.B., Na R.H., Wei S.S., Zhu J.S., Peng H.J. 2014. Distribution of tick-borne diseases in China. Parasties & Vectors, 6, 119. [Crossref]

  • Xu X.L., Cheng T.Y., Yang H., Yan F. 2015. Identification of intestinal bacterial flora in Rhipicephalus microplus ticks by conventional methods and PCR-DGGE analysis. Experimental and Applied Acarology, 66, 257–268. [Crossref]

  • Xu X.L., Cheng T.Y., Yang H., Liao Z.H. 2016. De novo assembly and analysis of midgut transcriptome of Haemaphysalis flava and identification of genes involved in blood digestion, feeding and defending from pathogens. Infectious Genetics and Evolution, 38, 62–72. [Crossref]

About the article

Received: 2016-06-14

Revised: 2016-12-21

Accepted: 2016-12-30

Published Online: 2017-04-18

Published in Print: 2017-06-01


Competing interestsThe authors declare that they have no competing interests.

Ethic statementAll animals were handled in strict accordance with the Good Animal Practice requirements of the Animal Ethics Procedures and Guidelines of the People’s Republic of China.


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0034. Export Citation

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