Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

4 Issues per year

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

See all formats and pricing
More options …
Volume 62, Issue 2


First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata

Anshu Chaudhary
  • Corresponding author
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), India- 250004
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Richa Tripathi
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), India- 250004
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shivi Gupta
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), India- 250004
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hridaya Shanker Singh
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), India- 250004
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-04-18 | DOI: https://doi.org/10.1515/ap-2017-0046


Freshwater snakehead fish, Channa punctata (Perciformes: Channidae) from Meerut (U.P.), India were screened for infection with metacercaria from the eye (vitreous humor) and brain (cranial cavity) and were analyzed by molecular methods using PCR and sequencing of the complete internal transcribed spacers (ITS1-5.8S-ITS2) of the ribosomal RNA gene. Recovered metacercariae from sites, vitreous humor and cranial cavity were previously diagnosed as Diplostomulum cerebralis Chakrabarti, 1968 on the basis of morphological characteristics. A combination of molecular methods in this study depicts that this is a species of genus Tylodelphys Diesing, 1850 which was misinterpreted as Diplostomulum. Hence, in this study, we validated the status of T. cerebralis (=D. cerebralis) after 49 years from its original description. The present work might contribute to expand our knowledge for identification, biodiversity and taxonomy of diplostomids in Indian fishes.

Keywords: Digenea; Tylodelphys; molecular study; rDNA; Meerut; India


  • Agrawal N., Khan S. 1982. A new Diplostomulum Brandes, 1892 metacercaria from the fish Heteropneutes fossilis. Indian Journal of Parasitology, 6, 81–83Google Scholar

  • Blasco-Costa I., Poulin R., Presswell, B. 2016. Morphological description and molecular analyses of Tylodelphys sp. (Trematoda: Diplostomidae) newly recorded from the freshwater fish Gobiomorphus cotidianus (common bully) in New Zealand. Journal of Helminthology, 1–14. CrossrefGoogle Scholar

  • Buchmann K., Uldal A. 1994. Effects of eyefluke infections on growth of rainbow trout (Oncorhynchus mykiss) in a mariculture system. Bulletin of the European Association of Fish Pathologists, 14, 104–107Google Scholar

  • Cavaleiro F.I., Pina S., Russell-Pinto F., Rodrigues P., Formigo N.E., Gibson D.I., Santos M.J. 2012. Morphology, ultrastructure, genetics, and morphometrics of Diplostomum sp. (Digenea: Diplostomidae) metacercariae infecting the European flounder, Platichthys flesus (L.) (Teleostei: Pleuronectidae), off the northwest coast of Portugal. Parasitology Research, 110, 81– 93. CrossrefGoogle Scholar

  • Chakrabarti K.K. 1968. A new strigeid metacercaria Diplostomulum cerebralis n. sp. from Indian freshwater fish. Zoologischer Anzeiger, 181, 307–312Google Scholar

  • Chakrabarti K.K., Baugh S.C. 1973. Studies on some strigeid metacercariae of the larval genus Diplostomulum Brandes, 1892 from Indian freshwater fishes. Revista Ibérica de Parasitología, 33, 107–125Google Scholar

  • Chappell L.H. 1995. The biology of diplostomatid eyeflukes of fishes. Journal of Helminthology, 69, 97–101. CrossrefGoogle Scholar

  • Chibwana F.D., Blasco-Costa I., Georgieva S., Hosea K.M., Nkwengulila G., Scholz T., Kostadinova A. 2013. A first insight into the barcodes for African diplostomids (Digenea: Diplostomidae): brain parasites in Clarias gariepinus (Siluriformes: Clariidae). Infection Genetics and Evolution, 17, 62–70. CrossrefGoogle Scholar

  • Chibwana F.D., Nkwengulila G. 2010. Variation in the morphometrics of diplostomid metacercariae (Digenea: Trematoda) infecting the catfish, Clarias gariepinus in Tanzania. Journal of Helminthology, 84, 61–70. CrossrefGoogle Scholar

  • Criscione C.D., Poulin R., Blouin M.S. 2005. Molecular ecology of parasites: elucidating ecological and microevolutionary processes. Molecular Ecology, 14, 2247–2257. CrossrefGoogle Scholar

  • Field J.S., Irwin S.W.B. 1995. Life-cycle description and comparison of Diplostomum spathaceum (Rudolphi, 1819) and D. pseudobaeri (Razmaskin & Andrejak, 1978) from rainbow trout (Oncorhynchus mykiss Walbaum) maintained in identical hosts. Parasitology Research, 81, 505–517. CrossrefGoogle Scholar

  • Galazzo D.E., Dayanandan S., Marcogliese D.J., McLaughlin J.D. 2002. Molecular systematics of some North American species of Diplostomum (Digenea) based on rDNA-sequence data and comparisons with European congeners. Canadian Journal of Zoology, 80, 2207–2217. CrossrefGoogle Scholar

  • Ganapati P.N., Rao K.H. 1954. On black-grub disease in the freshwater carp Catla catla. Current Science, 23, 401–402.Google Scholar

  • Gibson D.I. 1996. Trematoda. Part IV. In: (Eds L. Margolis and Z. Kabata) Guide to the parasites of fishes of Canada. Canadian Special Publication of Fisheries and Aquatic Sciences No. 124, NRC Press, Ottaw, 1–373Google Scholar

  • Grobbelaar A., Van As L., Van As Jo G., Butler H. 2015. Pathology of eyes and brain of fish infected with diplostomids, Southern Africa. African Zoology, 50, 181–186. CrossrefGoogle Scholar

  • Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Google Scholar

  • Larsen A.H., Bresciani J., Buchmann K. 2005. Pathogenicity of Diplostomum cercariae in rainbow trout, and alternative measures to prevent diplostomosis in fish farms. Bulletin of the European Association of Fish Pathologists, 25, 332–339Google Scholar

  • Locke S.A., Al-Nasiri F.S., Caffara M., Drago F., Kalbe M., Lapierre A.R., McLaughlin J.D., Nie P., Overstreet R.M., Souza G.T., Takemoto R.M., Marcogliese, D.J. 2015. Diversity, specificity and speciation in larval Diplostomidae (Platyhelminthes: Digenea) in the eyes of freshwater fish, as revealed by DNA barcodes. International Journal for Parasitology, 13, 841–855. CrossrefGoogle Scholar

  • Locke S.A., McLaughlin J.D., Dayanandan S., Marcogliese D.J. 2010. Diversity and specificity in Diplostomum spp. metacercariae in freshwater fishes revealed by cytochrome c oxidase I and internal transcribed spacer sequences. International Journal for Parasitology, 40, 333–343. CrossrefGoogle Scholar

  • Luton K., Walker D., Blair D. 1992. Comparison of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminthes: Trematoda: Digenea). Molecular and Biochemical Parasitology, 56, 323–328. CrossrefGoogle Scholar

  • Mehra H.R. 1962. Two new species of the genus Tylodelphys Diesing, 1850 (Diplostomatidae) from Indian birds. Proceedings of the National Academy of Sciences, India, Section B, Biological Sciences, 32, 417–420Google Scholar

  • Milne I., Lindner D., Bayer M., Husmeier D., McGuire G., Marshall D.F., Wright F. 2009. TOPALi v2: as rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multicore desktops. Bioinformatics, 25, 126–127. CrossrefGoogle Scholar

  • Mukherjee M., Srivastava C.B. 1989. Studies on larval strigeoid genus Diplostomulum Brandes, 1898, with description of a new species. Dr. B.S. Chauhan commemoration volume, 13–17Google Scholar

  • Niewiadomska K., Laskowski Z. 2002. Systematic relationships among six species of Diplostomum Nordmann, 1832 (Digenea) based on morphological and molecular data. Acta Parasitologica, 47, 20–28Google Scholar

  • Niewiadomska K., Niewiadomska-Bugaj M. 1995. Optimal identification procedure for Diplostomum paracaudum (Iles, 1959) and D. pseudospathaceum Niewiadomska, 1984 metacercariae (Digenea) based on morphological characters. Systematic Parasitology, 30, 165–171. CrossrefGoogle Scholar

  • Otachi E.O., Locke S.A., Jirsa F., Fellner-Frank C., Marcogliese D.J. 2015. Morphometric and molecular analyses of Tylodelphys sp. metacercariae (Digenea: Diplostomidae) from the vitreous humour of four fish species from Lake Naivasha, Kenya. Journal of Helminthology, 89, 404–414. CrossrefGoogle Scholar

  • Pandey K., Tewari S. 1984. A redescription of Diplostomum ophthalmi Pandey, 1968; with a note on validity of certain other Indian species. Uttar Pradesh Journal of Zoology, 4, 179–184Google Scholar

  • Pandey K.C. 1968. Studies on freshwater fishes of India III. On two new species of Diplostomulum Brandes, 1892. Proceedings of the Indian Academy of Science, 72, 167–170Google Scholar

  • Pandey K.C. 1970. Studies on metacercaria of freshwater fishes of India. III On two new species of Diplostomulum Brandes, 1892. Proceedings of the Indian Academy of Science, 42, 162–170Google Scholar

  • Pandey K.C. 1973. Studies on metacercariae of freshwater fishes of India. II. Description of a known and five unknown stregeid metacercariae from Lucknow. Indian Journal of Zoology, 14, 155–166Google Scholar

  • Pandey K.C., Agrawal N. 2013. Metacercarial Fauna of India. Records of Zoological Survey of India, Occassional Paper No., 349, Published by the Director, Zoological Survey of India, Kolkata, pp. 310Google Scholar

  • Rosser T.G., Alberson N.R., Khoo L.H., Woodyard E.T., Pote L.M., Griffin M.J. 2016. Characterization of the life cycle of a fish eye fluke, Austrodiplostomum ostrowskiae (Digenea: Diplostomidae), with notes on two other diplostomids infecting Biomphalaria havanensis (Mollusca: Planorbidae) from catfish aquaculture ponds in Mississippi, USA. Journal of Parasitology, 102, 260– 274. CrossrefGoogle Scholar

  • Shigin A.A. 1986. Trematode fauna of the USSR. Genus Diplostomum Metacercariae. Nauka, Moscow, 252 pp (In Russian)Google Scholar

  • Singh K.S. 1956. On some Strigeids from India. Journal of Zoological Society of India, 8, 47–56Google Scholar

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725–2729. CrossrefGoogle Scholar

  • Thapar G.S. 1967. Some new larval strigeids causing black spot disease in fishes from Tungabhadra dam. Indian Journal of Helminthology, 19, 173–182Google Scholar

  • Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680Google Scholar

About the article

Received: 2016-09-14

Revised: 2017-01-17

Accepted: 2017-01-18

Published Online: 2017-04-18

Published in Print: 2017-06-01

Citation Information: Acta Parasitologica, Volume 62, Issue 2, Pages 386–392, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0046.

Export Citation

© 2017 W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in