Jump to ContentJump to Main Navigation
Show Summary Details
In This Section

Acta Parasitologica

4 Issues per year

IMPACT FACTOR 2016: 1.160
5-year IMPACT FACTOR: 1.185

CiteScore 2016: 1.24

SCImago Journal Rank (SJR) 2015: 0.581
Source Normalized Impact per Paper (SNIP) 2015: 0.780

See all formats and pricing
In This Section
Volume 62, Issue 2 (Jun 2017)


Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages

Carmen Guzmán-Cornejo
  • Corresponding author
  • Laboratorio de Acarología, Facultad de Ciencias, Departamento de Biología Comparada, Universidad Nacional Autónoma de México. D.F., México
  • Email:
/ Luis García-Prieto
  • Colección Nacional de Helmintos, Instituto de Biología, Universidad Nacional Autónoma de México, México, D.F., México
/ Andrea Rebollo-Hernández
  • Laboratorio de Acarología, Facultad de Ciencias, Departamento de Biología Comparada, Universidad Nacional Autónoma de México. D.F., México
/ José M. Venzal
  • Instituto Nacional de Tecnología Agropecuaria and Consejo Nacional de Investigaciones Científicas y Técnicas, Rafaela, Santa Fe, Argentina
/ Santiago Nava
  • Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Salto, Uruguay
/ Sokani Sánchez-Montes
  • Laboratorio de Acarología, Facultad de Ciencias, Departamento de Biología Comparada, Universidad Nacional Autónoma de México. D.F., México
Published Online: 2017-04-18 | DOI: https://doi.org/10.1515/ap-2017-0051


Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species is validated on the basis of molecular evidence (using partial sequences of 16S rDNA gene), and the morphological differences between them (dentition of the hypostome and traits of individual mammillae) are confirmed through light and scanning electron microscopy. In addition to the above characteristics, we observed other traits that may serve to differentiate both species: dorsal setae are short and thick in O. yumatensis and are thin and moderate in size in O. brodyi. We also observed a conspicuous hood in O. brodyi, which was absent in O. yumatensis. Another characteristic observed is a line of setae, near the end of Tarsi II-IV, which in O. brodyi is formed by less than five setae and in O. yumatensis by more than five. The main morphological difference between larvae of the 2 species is the number of ventral setae [9 (4 circumanal pairs) in O. brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic divergence in 16S rDNA sequences between these two species ranges from 9.7 to 10.6%.

Keywords: Ticks; Argasids; Mexico; caves; morphology; DNA


  • Apanaskevich D., Oliver Jr. 2014. Life cycles and Natural History of Ticks. In. Sonenshine D.E., Roe R.M. (Eds) Biology of Ticks. N.Y. Oxford University Press. 59–73

  • Cooley R.A., Kohls G.M. 1941. Three New Species of Ornithodoros (Acarina: Ixodoidea). Public Health Reports, 56, 587–594. [Crossref]

  • Cooley R.A., Kohls G.M. 1944. The Argasidae of North America, Central America and Cuba. The American Midland Naturalist. Monograph No. 1, pp.152

  • Clifford C.M., Kohls G.M., Sonenshine D.E. 1964. The systematic of the subfamily Ornithodorinae (Acarina: Argasidae). I. The genera and subgenera. Annals of the Entomological Society of America, 57, 429–437. [Crossref]

  • Dooley T., Bristol J., Canaris A. 1976. Ectoparasites from Bats in Extreme West Texas and South-Central New Mexico. Journal of Mammalogy, 57, 189–191. [Crossref]

  • Estrada-Peña A., Venzal J.M., Kocan K. M., Tramuta C., Tomassone L., de la Fuente J., Labruna M. 2008. Observations on Antricola Ticks: Small Nymphs Feed on Mammalian Hosts and Have a Salivary Gland Structure Similar to Ixodid Ticks. Journal of Parasitology, 94, 953–955. [Crossref]

  • Gannon W.L., Sikes, R. S. 2007. Guidelines of the American Society of Mammalogists for the use of wild mammals in research. Journal of Mammalogy, 88, 809–823 [Crossref]

  • Hall T.A. 1999. Bioedit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98

  • Jones E.K., Clifford C.M., Keirans J.E., Kohls G.M. 1972. The ticks of Venezuela (Acarina: Ixodoidea) with a key to the species of Amblyomma in the Western Hemisphere. Brigham Young University Science Bulletin, Biological Series, 17, 1–40

  • Kohls G.M., Sonenshine D.E., Clifford C.M. 1965. The systematics of the subfamily Ornithodorinae (Acarina: Argasidae). II. Identification of the larvae of the Western Hemisphere and description of three new species. Annals of the Entomological Society of America, 58, 331–364

  • Matheson R. 1935. Three new species of ticks, Ornithodoros (Acarina: Ixodoidea). Journal of Parasitology, 21, 347–353. [Crossref]

  • Norris D.E., Klompen J.S.H., Keirans J.E., Black W.C. 4th. 1996. Population genetics of Ixodes scapularis (Acari: Ixodidae) based on mitochondrial 16S and 12S genes. Journal of Medical Entomology, 33, 78–89. [Crossref]

  • Pence D.B., Knoxjones J., Knipping P.A. 1981. Acari of Antillean bats (Chiroptera). Journal of Medical Entomology, 18, 353– 354. [Crossref]

  • STATSOFT, INC. 2009. Statistica V.8. Tulsa, Oklahoma, USA

  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution. [Crossref]

  • Thompson J.D., Higgins D., Gibson T.J. 1994. CLUSTAL W: improving the sensivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680. [Crossref]

  • Trout R.T., Steelman C.D., Szalanski A.L. 2009. Population genetics and phylogeography of Ixodes scapularis from canine and deer in Arkansas. Southwestern Entomologist, 34, 273–287. [Crossref]

  • Venzal J.M., Nava S., Terassini F.A., Ogrzewalska M., Camargo L.M.A., Labruna M.B. 2013. Ornithodoros peropteryx (Acari: Argasidae) in Bolivia: an argasid tick with a single nymphal stage. Experimental and Applied Acarology, 61, 231–241. [Crossref]

  • Villegas-Guzman G.A., López-González C., Vargas M. 2005. Ectoparasites associated to two species of Corynorhinus (Chiroptera: Vespertilionidae) from Guanaceví Mining Region, Durango, Mexico. Journal of Medical Entomology, 42, 125– 127. [Crossref]

  • Wackerly D., Mendenhall W., Scheaffer R.L. 2014. Mathematical Statistics with Applications. Cengage Learning, Boston, pp. 944

About the article

Received: 2016-08-17

Revised: 2017-02-09

Accepted: 2017-02-23

Published Online: 2017-04-18

Published in Print: 2017-06-01

Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0051. Export Citation

Comments (0)

Please log in or register to comment.
Log in