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Acta Parasitologica

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Volume 62, Issue 2 (Jun 2017)

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Toxoplasma gondii in backyard pigs: seroepidemiology and mouse bioassay

Erika Fernanda Torres Samico-Fernandes
  • Corresponding author
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ Marcela Fernanda Torres Samico-Fernandes
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ Pedro Paulo Feitosa de Albuquerque
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ Jonatas Campos de Almeida
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ André de Souza Santos
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ André da Rocha Mota
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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/ Orestes Luiz de Souza Neto
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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  • De Gruyter OnlineGoogle Scholar
/ Rinaldo Aparecido Mota
  • Rua Dom Manoel de Medeiros s/n. Dois Irmãos, Recife – PE. Brazil. CEP: 52171-900. Laboratório de Doenças Infectocontagiosas, Departamento de Medicina Veterinária/Universidade Federal Rural de Pernambuco, Brazil
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Published Online: 2017-04-18 | DOI: https://doi.org/10.1515/ap-2017-0054

Abstract

The aim of this study was to evaluate the occurrence of Toxoplasma gondii in backyard pigs destined for human consumption in Pernambuco state, Brazil. Blood and tissue samples (liver, heart, brain, lung and diaphragm) were collected from 224 pigs from legal slaughterhouses and tested for T. gondii infection. Antibodies to T. gondii were found in the sera of 37.9% (85/224) by using the immunofluorescence antibody test (cut-off – 1:64). Tissue samples from seropositive pigs were bioassayed in mice. Tissue samples from seropositive pigs and from mice of the bioassay were submitted to histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequencing; 14.1% of pig tissue samples and 27.7% of bioassayed mouse samples were positive for T. gondii DNA, but all pig and mouse tissues were negative in histopathology analysis and immunochemistry. By using a risk assessment questionnaire, there was significant difference (p<0.001) in seroprevalence of 21.2% (reproducer) and 3.1% (finishing pig). These data serve as indicative of the sanitary conditions and risk of T. gondii infection for backyard pigs. Preventive measures must be implemented by health services to avoid toxoplasmosis human cases due to ingestion of pig meat.

Keywords: Backyard pigs; direct diagnosis; serology; sequencing; Toxoplasma gondii

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About the article

Received: 2016-11-29

Revised: 2017-01-20

Accepted: 2017-02-20

Published Online: 2017-04-18

Published in Print: 2017-06-01


Conflict of interest statement The authors declare that no competing interests exist.


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0054.

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© 2017 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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