Abstract
Naturally Leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. Vaginal secretions were collected for polymerase chain reaction (PCR), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (IHQ) identification of L. infantum. Parasite DNA was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (P<0.05; Fisher’s Exact test). IHQ was positive in vulvar dermis (23.5%) and vaginal mucosa (17.7%) but negative in endometrium of all bitches. Poor association between positive vaginal secretion PCR and tissue IHQ (Kappa index) were observed. The results showed that genital secretions are a potential source for dog contamination.
Acknowledgments
Funding: This work was supported by the Fundo de Incentivo á Pesquisa da Pontificia Universidade Católica de Minas Gerais (FIP/PUC Minas) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico [grant number 2007/2399- S1], Brazil.
References
Alvar J., Vélez I.D. Bern C., Herrero M., Desjeux P., Cano J., et al. 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One, 7, e35671. 10.1371/journal.pone.0035671Search in Google Scholar PubMed PubMed Central
Boechat C.V., Mendes Júnior A.A.V., Madeira M.F., Ferreira L.C., Figueiredo F.B., Rodrigues F.C.C., et al. 2016. Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs. Parasitology Research, 115, 2371–2379. 10.1007/s00436-016-4987-4Search in Google Scholar PubMed
Ciaramella P., Oliva G., Luna R.D., Gradoni L., Ambrosio R., Cortese L., et al. 1997. A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. The Veterinary Record, 141, 539–54310.1136/vr.141.21.539Search in Google Scholar PubMed
Concannon P.W., Dillingham L., Spitz I.M. 1988. Effects of the antiprogestin RU486 on progesterone-dependent uterine development and bioassay of progestational activity in estrogen-primed immature female dogs. Acta Endocrinologica, 118, 389–39810.1530/acta.0.1180389Search in Google Scholar PubMed
Coutinho M.T., Bueno L.L., Sterzik A., Fujiwara R.T., Botelho J.R., de Maria M., et al. 2005. Participation of Rhipicephalus sanguineus (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis. Veterinary Parasitology, 128, 149–155. 10.1016/j.vetpar.2004.11.011.Search in Google Scholar PubMed
Diniz S.A., Melo M.S., Borges A.M., Bueno R., Reis B.P., Tafuri W.L., et al. 2005. Genital lesions associated with visceral leishmaniasis and shedding of Leishmania sp. in the semen of naturally infected dogs. Veterinary Pathology, 42, 650–658. 10.1354/vp.42-5-650Search in Google Scholar PubMed
Dubey J.P., Rosypal A.C., Pierce V., Scheinberg S.N., Lindsay D.S. 2005. Placentitis associated with leishmaniasis in a dog. Journal of American Veterinary Medical Association, 227, 1266–126910.2460/javma.2005.227.1266Search in Google Scholar PubMed
Ferreira S.A., Ituassu L.T., Melo M.N., Andrade A.S.R. 2008. Evaluation of the conjunctival swab for canine visceral leishmaniasis diagnosis by PCR-hybridization in Minas Gerais State, Brazil. Veterinary Parasitology, 152, 257–263. 10.1016/j.vetpar.2007.12.022Search in Google Scholar PubMed
Freitas E., Melo M.N., Costa-Val A.P., Michalick M.S. 2006. Transmission of Leishmania infantum via blood transfusion in dogs: potential for infection and importance of clinical factors. Veterinary Parasitology, 137, 159–167. 10.1016/J.VETPAR.2005.12.011Search in Google Scholar PubMed
Gaskin A.A., Schantz P., Jackson J., Birkenheur A., Tomlinson L., Gramiccia M., et al. 2002. Visceral leishmaniasis in a New York foxhound kennel. Journal of Veterinary Internal Medicine, 16, 34–4410.1111/j.1939-1676.2002.tb01604.xSearch in Google Scholar
Karkamo V., Kaistinen A., Näreaho A., Dillard K., Vainio-Siukola K., Vidgrén G., et al. 2014. The first report of autochthonous non-vector-borne transmission of canine leishmaniasis in the Nordic countries. Acta Veterinaria Scandinavica, 56, 84. 10.1186/s13028-014-0084-9Search in Google Scholar PubMed PubMed Central
Landis J.R., Koch G.G. 1977. The measurement of observer agreement for categorical data. Biometrics, 33, 159–17410.2307/2529310Search in Google Scholar
Maret A., Coudert J.D., Garidou L., Foucras G., Gourdy P., Krust A., et al. 2003. Estradiol enhances primary antigen-specific CD4 T cells responses and Th1 development in vivo. Essential role of estrogen receptor alpha expression in hematopoietic cells. European Journal of Immunology, 33, 512–521. 10.1002/immu.200310027.Search in Google Scholar PubMed
Paltrinieri S., Gradoni L., Roura X., Zatelli A., Zini E. 2016. Laboratory tests for diagnosing and monitoring canine leishmaniasis. Veterinary Clinical Pathology, 45, 552–578. 10.1111/vcp.12413Search in Google Scholar PubMed
Pangrazio K.K., Costa E.A., Amarilla S.P., Cino A.G., Silva T.M., Paixão T.A., et al. 2009. Tissue distribution of Leishmania chagasi and lesions in transplacentally infected fetuses from symptomatic and asymptomatic naturally infected bitches. Veterinary Parasitology, 165, 327–331. 10.1016/j.vetpar.2009.07.013Search in Google Scholar PubMed
Papadogiannakis E.I., Koutinas A.F. 2015. Cutaneous immune mechanisms in canine leishmaniosis due to Leishmania infantum. Veterinary Immunology and Immunopathology, 163, 94–102. 10.1016/j.vetimm.2014.11.011Search in Google Scholar PubMed
Piarroux R., Azaiez R., Lossi A.M., Reynier P., Muscatelli F., Gambarelli F., et al.1993. Isolation and characterization of a repetitive DNA sequence of Leishmania infantum: development of visceral leishmaniasis polymerase chain reaction. The American Journal of Tropical Medicine and Hygiene, 49, 364–36910.4269/ajtmh.1993.49.364Search in Google Scholar PubMed
Pires M.A., Payan-Carreira R. 2015. Resident macrophages and lymphocytes in the canine endometrium. Reproduction in Domestic Animals, 50, 740–749. 10.1111/rda.12567Search in Google Scholar PubMed
Reguera R.M., Morán M., Pérez-Pertejo Y., García-Estrada C., Balaña-Fouce R. 2016. Current status on prevention and treatment of canine leishmaniasis. Veterinary Parasitology, 227, 98–114. 10.1016/j.vetpar.2016.07.011Search in Google Scholar PubMed
Root Kustritz M.V. 2005. Reproductive behavior of small animals. Theriogenology, 64, 734–746. 10.1016/j.theriogenology.2005.05.022Search in Google Scholar PubMed
Root Kustritz M.V. 2012. Managing the reproductive cycle in the bitch. Veterinary Clinics of North America: Small Animal Practice, 42, 423–437. 10.1016/j.cvsm.2012.01.012Search in Google Scholar PubMed
Silva L.C., Assis V.P., Ribeiro V.M., Tafuri W.L., Toledo Júnior J.C., Silva S.O., et al. 2014. Detection of Leishmania infantum in the smegma of infected dogs. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66, 731–736. 10.1590/1678-41626610Search in Google Scholar
Silva F.L., Oliveira R.G., Silva T.M.A., Xavier M.N., Santos R.L. 2009. Venereal transmission of canine visceral leishmaniasis. Veterinary Parasitology, 160, 55–59. 10.1016/j.vetpar.2008.10.079Search in Google Scholar PubMed
Silva F.L., Rodrigues A.A.M., Rego I.O.P., Oliveira R.G., Silva T.M., Xavier M.N., et al. 2008. Genital lesions and distribution of amastigotes in bitches naturally infected with Leishmania chagasi. Veterinary Parasitology, 151, 86–90. 10.1016/j.vetpar.2007.09.032Search in Google Scholar PubMed
Sugiura K., Nishikawa M., Ishiguro K., Tajima T., Inaba M., Torii R., et al. 2004. Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch. Immunobiology, 209, 619–627. 10.1016/j.imbio.2004.09.003Search in Google Scholar PubMed
Tafuri W.L., Santos R.L., Arantes R.M.E., Gonçalves R., Melo M.N., Michalick M.S.M., Tafuri W.L. 2004. An alternative immunohistochemical method for detecting Leishmania amastigotes in paraffin-embedded canine tissues. Journal of Immunology Methods, 292, 17–23. 10.1016/j.jim.2004.05.009Search in Google Scholar PubMed
Tammer I., Blendinger K., Sobiraj A., Bostedt H. 1994. The use of exfoliative vaginal cytology for the gynecological evaluation of the bitch. Tierärztliche Praxis, 22, 199–207Search in Google Scholar
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