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Acta Parasitologica

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Volume 62, Issue 3 (Sep 2017)

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Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches

Alinne Gonçalves Magro
  • Departamento de Medicina Veterinária da Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário 1081, Bairro Angola, Betim, Minas Gerais, 32604-115, Brazil
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/ Viviane Pedersoli Assis
  • Departamento de Medicina Veterinária da Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário 1081, Bairro Angola, Betim, Minas Gerais, 32604-115, Brazil
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/ Lucélia Coimbra Silva
  • Departamento de Medicina Veterinária da Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário 1081, Bairro Angola, Betim, Minas Gerais, 32604-115, Brazil
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/ Wagner Luiz Tafuri
  • Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil
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/ Soraia Oliveira Silva
  • Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil
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/ Maria Norma Melo
  • Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil
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/ Guilherme Ribeiro Valle
  • Corresponding author
  • Departamento de Medicina Veterinária da Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário 1081, Bairro Angola, Betim, Minas Gerais, 32604-115, Brazil
  • Programa de Pós-graduação em Biologia de Vertebrados da Pontifícia Universidade Católica de Minas Gerais, Av. Dom José Gaspar 500, prédio 41, Bairro Coração Eucarístico, Belo Horizonte, Minas Gerais, 30140-100, Brazil
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Published Online: 2017-07-05 | DOI: https://doi.org/10.1515/ap-2017-0076

Abstract

Naturally Leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. Vaginal secretions were collected for polymerase chain reaction (PCR), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (IHQ) identification of L. infantum. Parasite DNA was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (P<0.05; Fisher’s Exact test). IHQ was positive in vulvar dermis (23.5%) and vaginal mucosa (17.7%) but negative in endometrium of all bitches. Poor association between positive vaginal secretion PCR and tissue IHQ (Kappa index) were observed. The results showed that genital secretions are a potential source for dog contamination.

Keywords: Leishmania infantum; bitch; genital secretions

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About the article

Received: 2016-12-29

Revised: 2017-05-02

Accepted: 2017-05-08

Published Online: 2017-07-05

Published in Print: 2017-09-26


Citation Information: Acta Parasitologica, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0076.

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© 2017 W. Stefański Institute of Parasitology, PAS. Copyright Clearance Center

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