Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2018: 1.00

SCImago Journal Rank (SJR) 2018: 0.500
Source Normalized Impact per Paper (SNIP) 2018: 0.664

More options …
Volume 62, Issue 3


Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences

Chandni Verma
  • Corresponding author
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), - 250004 India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anshu Chaudhary
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), - 250004 India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hridaya Shanker Singh
  • Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.), - 250004 India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-07-05 | DOI: https://doi.org/10.1515/ap-2017-0079


The phylogenetic studies on monogeneans of the genus Thaparocleidus parasitizing W. attu in India was inferred from 18S rDNA gene data. Out of species of Thaparocleidus, one new, T. armillatus sp. n., is described herein which distinguished from its congener species in the shapes and sizes of sclerotized structures and rest of the two species, T. indicus and T. wallagonius are redescribed morphologically as well as validated and confirmed by molecular means also. Besides the above, T. gangus was also included in the molecular analysis as it is recently described on the basis of morphology only. The phylogenetic tree was also reconstructed in order to elucidate the taxonomic status of Thaparocleidus armillatus sp. n., T. indicus, T. wallagonius and T. gangus. This research reports for the first time, the molecular phylogenetic analysis of 18S rDNA gene for four species belonging to the genus Thaparocleidus from W. attu collected off the River Ganga in India. In phylogram, all four species of Thapaocleidus come in a single clade that confirmed their monophyletic status. A very low interspecific variability was observed in T. armillatus sp. n. and T. wallagonius suggesting that these species are highly similar in morphology. Correspondingly, T. indicus and T. gangus also showed low interspecific variability which too signifies their morphological similarities in their sclerotized structures. Our result suggested that all four species taken in the present investigation from India are monophyletic and it is also observed that morphological similarities in the sclerotized structures are well correlated with phylogeny.

Keywords: Monogenea; Thaparocleidus spp.; morphology; molecular; 18S rDNA; India


  • Akhmerov A.K. 1964. On the evolution of the median attachment apparatus of the monogenetic suborder Dactylogyrinea. Trudy Gel’minthologicheskoi Laboratorii, 14, 69–79 (In Russian)Google Scholar

  • Agrawal N., Sharma R. 1988. On a new monogenea Neomurraytrema lucknowensis sp. nov. from Mystus vittatus Bloch). Indian Journal of Parasitology, 12, 61–63Google Scholar

  • Agrawal N., Vishwakarma, P., Shukla R., Devak A. 2005. A note on surface topography of Thaparocleidus indicus (Kulkarni, 1969) Lim, 1966 on Wallago attu (Bloch and Schn.). National Journal of Life Science, 2, 131–132Google Scholar

  • Caballero Y.C.E., Bravo-Hollis, M. 1961. Trematodes de peces de aguas Mexicanas del Pacifico. XVIII. Un nuevo género y una nueva especie de Monogenoidea Byjovskii, 1937. Anales del Instituto de Biología, 31, 197–205. (In Spanish)Google Scholar

  • Chaudhary A., Singh H.S. 2012. Phylogenetic study of nine species of freshwater monogeneans using secondary structure and motif prediction from India. Bioinformation, 8, 862–869CrossrefGoogle Scholar

  • Chaudhary A., Singh H.S. 2012. Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence. Journal of Helminthology, 87, 160–173CrossrefGoogle Scholar

  • Chaudhary A., Verma C., Varma M., Singh H.S. 2014. Identification of Thaparocleidus caecus (Monogenea: Dactylogyridae) using morphological and molecular tools: a parasite invasion in Indian freshwater. Bioinvasions Records, 3, 195–200Google Scholar

  • Dubey A., Gupta A.K., Agarwal S.M. 1992. Studies on monogenean parasites in freshwater fishes at Raipur. VIII. Validity of Parancylodiscoides Akhmerov, 1964 (Silurodiscoides Gusev, 1974) and two new species from Raipur. Indian Journal of Helminthology, 44, 9–16Google Scholar

  • Froese R., Pauly D. 2002. Fish Base: World Wide Web electronic publication. www.fishbase.org

  • Gusev A.V. 1973. Monogeneans of freshwater fishes of India and an analysis of the world fauna of the group. Abstract of Doctor of Biological Science Thesis, Leningrad: Akademiya Nauk, pp. 31Google Scholar

  • Gusev A.V. 1976. Freshwater Indian Monogenoidea. Principles of systematics, analysis of the world faunas and their evolution. Indian Journal of Helminthology, 25 and 26 (1973 and 1974), 1–241Google Scholar

  • Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Google Scholar

  • Jain S.L. 1952. Monogenea of Indian freshwater fishes. II. Thaparocleidus wallagonius n.g. and n. sp. (subfamily Tetraonchinae) from the gills of Wallagonia attu (Bloch) from Lucknow. Indian Journal of Helminthology, 4, 43–48Google Scholar

  • Kulkarni T. 1969. On a new species Ancylodiscoides Yamaguti, 1937 from the fish Wallagonia attu (Bloch) in Hyderabad, A.P. Zoologische Anzeiger, 182, 461–465Google Scholar

  • Lim L.H.S. 1996. Thaparocleidus Jain, 1952, the senior synonym of Silurodiscoides Gussev, 1976 (Monogenea: Ancylodiscoidinae). Systematic Parasitology, 35, 207–215Google Scholar

  • Lim L.H.S. 1998. Diversity of monogeneans in Southeast Asia. International Journal of Parasitology, 28, 1495–1515CrossrefGoogle Scholar

  • Lim L.H.S., Lerssutthichawal T. 1996. Monogeneans from Wallago attu (Bloch and Schneider, 1802) of Thailand. Raffles Bulletin of Zoology, 44, 287–300Google Scholar

  • Lim L.H.S., Timofeeva T.A., Gibson D.I. 2001. Dactylogyridean monogeneans of the siluriform fishes of the Old World. Systematic Parasitology, 50, 159–197Google Scholar

  • Littlewood D.T.J., Olson P.D. 2001. Small subunit rDNA and the phylum Platyhelminthes: signal, noise, conflict and compromise. In: (Eds Littlewood, D.T.J., Bray, R.A.) Interrelationships of the Platyhelminthes. Taylor and Francis, London, U.K., pp. 262–278Google Scholar

  • Mendoza-Palmero C.A., Blasco-Costa I., Scholz T. 2015. Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasites & Vectors, 8, 164CrossrefWeb of ScienceGoogle Scholar

  • Milne I., Lindner D., Bayer M., Husmeier D., McGuire G., Marshall D.F., Wright F. 2009. TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics, 25, 126–127CrossrefWeb of ScienceGoogle Scholar

  • Pandey K.C., Agarwal A. 1989. On a new monogenetic trematoda from Wallago attu (Bl.). Indian Journal of Helminthology (N.S.), 5, 51–53Google Scholar

  • Pandey K.C., Agarwal N. 2008. An encyclopaedia of Indian monogenoidea. Vitasta Publishing Pvt. Ltd., New Delhi, India, pp. 522Google Scholar

  • Pandey K.C., Mehta T. 1986. Studies on some new monogenetic trematodes of Wallago attu (Bloch) at Meerut, Uttar Pradesh, India. In: Proceedings of the Indian National Convocation for Young Scientists, 114–132Google Scholar

  • Posada D., Crandall K.A. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics, 14, 817–818CrossrefGoogle Scholar

  • Šimková A., Serbielle C., Pariselle A., Vanhove M.P.M., Morand S. 2013. Speciation in Thaparocleidus (Monogenea: Dactylogyridae) parasitizing Asian pangasiid catfishes. BioMed Research International, 353956. CrossrefGoogle Scholar

  • Singh H.S., Sharma B. 1992. Wallagotrema indicus n. sp., a new monogenean from the freshwater shark Wallago attu (Bloch and Schn). Functional Development and Morphology, 2, 85–88Google Scholar

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular evolution genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729Google Scholar

  • Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680CrossrefGoogle Scholar

  • Tripathi Y.R. 1959. Monogenetic trematodes from fishes of India. Indian Journal of Helminthology, 9, 1–149Google Scholar

  • Van de Peer Y., De Rijk P., Wuyts J., Winkelmans T., De Wachter R. 2000. The European Small Subunit Ribosomal RNA database. Nucleic Acids Research, 28, 175–176.CrossrefGoogle Scholar

  • Verma C., Chaudhary A., Singh H.S. 2015. Morphological redescription and first molecular analyses of two species of Mizelleus (Monogenea, Dactylogyridae) infecting the Indian Gangetic shark, Wallago attu. Journal of Helminthology 16, 1–11. CrossrefGoogle Scholar

  • Verma C., Chaudhary A., Singh H.S. 2016. Thaparocleidus gangus sp. nov. (Monogenea: Dactylogyridae) from gill filaments of Wallago attu Bloch and Schn., 1801, India. Turkish Journal of Zoology, 40: 758–764. CrossrefGoogle Scholar

About the article

Received: 2016-10-21

Revised: 2017-05-12

Accepted: 2017-05-18

Published Online: 2017-07-05

Published in Print: 2017-09-26

Citation Information: Acta Parasitologica, Volume 62, Issue 3, Pages 652–665, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0079.

Export Citation

© 2017 W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in