Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

More options …
Volume 62, Issue 4


The molecular identification of Calodium hepaticum in the wild brown rat (Rattus norvegicus) in Poland

Katarzyna Buńkowska-Gawlik
  • Corresponding author
  • Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Agnieszka Perec-Matysiak
  • Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Klaudia Burzyńska
  • Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Joanna Hildebrand
  • Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63, 51-148 Wrocław, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-10-15 | DOI: https://doi.org/10.1515/ap-2017-0087


Calodium hepaticum is a parasitic nematode found primarily in rodents but known to infect numerous other mammal species, and causing in humans the hepatic calodiasis. Herein, we present the first finding of C. hepaticum in Rattus norvegicus in Poland. In this study, we have used the combined pepsin-HCl digestion and molecular techniques to detect C. hepaticum infection in the liver. The results confirm the usefulness of molecular approaches for investigating the biology and epidemiology of C. hepaticum. Additionally in this study, the parts of the small subunit rRNA (18S rRNA) gene of Aonchotheca annulosa from bank vole, Aonchotheca erinacei and Eucoleus sp. from hedgehog were amplified, yielding the first 18S rRNA gene sequences of these Capillariinae nematodes.

Keywords: Calodium hepaticum; zoonotic; Rattus norvegicus; molecular analysis


  • Baker D.G. 2006. Parasitic diseases. In: (Eds. Suckow M.A., Weisbroth S.H., Franklin C.L.) The laboratory rat. Academic Press, San Diego, 453–478Google Scholar

  • Carvalho-Costa F.A, Silva A.G., Souza A.H., Moreira C.J., Souza D.L., Valverde J.G., et al. 2009. Pseudoparasitism by Calodium hepaticum (syn. Capillaria hepatica; Hepaticola hepatica) in the Negro River, Brazilian Amazon. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103, 1071–1073. CrossrefGoogle Scholar

  • Ceruti R., Sonzogni O., Origgi F., Vezzoli F., Cammarata S., Giusti A.M., Scanziani E. 2001. Capillaria hepatica infection in wild brown rats (Rattus norvegicus) from the urban area of Milan, Italy. Journal of veterinary medicine. B, Infectious diseases and veterinary public health, 48, 235–240PubMedCrossrefGoogle Scholar

  • Choe G., Lee H.S., Seo J.K., Chai J.Y., Lee S.H., Eom K.S., Chi J.G. 1993. Hepatic capillariasis: first case report in the Republic of Korea. The American Journal of Tropical Medicine and Hygiene, 48, 610–625PubMedCrossrefGoogle Scholar

  • Cheesbrough M. (Ed.). 1998. District Laboratory Practice in Tropical Countries. Part 1. Cambridge University Press, London, pp. 456Google Scholar

  • Claveria F.G., Causapin J., de Guzman M.A., Toledo M.G., Salibay C. 2005. Parasite biodiversity in Rattus spp. caught in wet markets. The Southeast Asian Journal of Tropical Medicine and Public Health, 36, 146–148PubMedGoogle Scholar

  • Debenedetti Á.L., Sáez-Durán S., Sainz-Elipe S., Galán-Puchades M.T., Fuentes M.V. 2014. Hepatic parasitosis in two wood mice, Apodemus sylvaticus (Rodentia: Muridae), due to Aonchotheca annulosa (Nematoda: Trichuridae), and Eucoleus bacillatus (Nematoda: Trichuridae). Erratic parasitism or post mortem migration? Acta Parasitologica, 59, 610–614. CrossrefGoogle Scholar

  • de Quadros R.M., Weiss P.H.E., Miletti L.C., de Moura A.B. 2016. Occurrence of Calodium hepaticum (Bancroft, 1893) Moravec, 1982 eggs in feces of dogs and cats in Lages, Santa Catarina, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, 58, 6. CrossrefWeb of ScienceGoogle Scholar

  • Easterbrook J.D., Kaplan J.B., Vanasco N.B., Reeves W.K., Purcell R.H., Kosoy M.Y., et al. 2007. A survey of zoonotic pathogens carried by Norway rats in Baltimore, Maryland, USA. Epidemiology and Infection, 135, 1192–1199. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Fuehrer H.P., Igel P., Auer H. 2011. Capillaria hepatica in man-an overview of hepatic capillariosis and spurious infections. Parasitology Research, 109, 969–979. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Fuehrer H.P. 2014a. An overview of the host spectrum and distribution of Calodium hepaticum (syn. Capillaria hepatica): part 1-Muroidea. Parasitology Research, 113, 619–640. CrossrefWeb of ScienceGoogle Scholar

  • Fuehrer H.P. 2014b. An overview of the host spectrum and distribution of Colodium hepaticum (syn. Capillaria hepatica): part 2-Mammalia (excluding Muroidea). Parasitology Research, 113, 641–651. CrossrefGoogle Scholar

  • Fugassa M.H., Taglioretti V., Goncalves M.L., Araujo A., Sardella N.H., Denegri G.M. 2008. Capillaria spp. eggs in Patagonian archaeological sites: statistical analysis of morphometric data. Memórias do Instituto Oswaldo Cruz, 103, 104–105PubMedCrossrefGoogle Scholar

  • Guardone L., Deplazes P., Macchioni F., Magi M., Mathis A. 2013. Ribosomal and mitochondrial DNA analysis of Trichuridae nematodes of carnivores and small mammals. Veterinary Parasitology, 197, 364–369. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Gonçalves A.Q., Ascaso C., Santos I., Serra P.T., Julião G.R., Orlandi P.P. 2012. Calodium hepaticum: household clustering transmission and the finding of a source of human spurious infection in a community of the Amazon region. PLoS Neglected Tropical Diseases, 6, 1–6. CrossrefWeb of ScienceGoogle Scholar

  • Kruminis-Łozowska W. 1984. Pasożyty wewnętrzne szczurów – Rattus norvegicus (Berk.) odławianych na terenie Trójmiasta. Materiały IX Zjazdu PTP, Wrocław – Zamek Książ 20–22 września. (In Polish)Google Scholar

  • Li C.D., Yang H.L., Wang Y. 2010. Capillaria hepatica in China. World Journal of Gastroenterology, 16, 698–702. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Macchioni F., Chelucci L., Guardone L., Mignone W., Prati M.C., Magi M. 2013. Calodium hepaticum (Nematoda: Capillaridae) in a red fox (Vulpes vulpes) in Italy with scanning electron microscopy of the eggs. Folia Parasitologica, 60, 102–104. CrossrefGoogle Scholar

  • McGarry J.W., Higgins A., White N.G., Pounder K.C., Hetzel U. 2015. Zoonotic helminths of urban brown rats (Rattus norvegicus) in the UK: neglected public health considerations? Zoonoses and Public Health, 62, 44–52. CrossrefWeb of SciencePubMedGoogle Scholar

  • Milazzo C., Ribas A., Casanova J.C., Cagnin M., Geraci F., Di Bella C. 2010. Helminths of the brown rat (Rattus norvegicus) (Berkenhout, 1769) in the city of Palermo, Italy. Helminthologia, 47, 238–240. CrossrefWeb of ScienceGoogle Scholar

  • Moravec F. 1982. Proposal of a new systematic arrangement of nematodes of the family Capillaridae. Folia Parasitologica, 29, 119–132Google Scholar

  • Moreira V.L.C., Giese E.G., da Silva D.C.B., de Vasconcelos Melo F.T., Furtado A.P., Maldonado A.Jr., dos Santos J.N. 2013. Calodium hepaticum (Nematoda: Capillariidae) in synanthropic rodents (Rattus norvegicus and Rattus rattus) in Eastern Amazonia. Revista Brasileira de Parasitologia Veterinária, 22, 265–269. CrossrefGoogle Scholar

  • Namue C., Wongsawad C. 1997. Survey of helminth infection in rats (Rattus spp) from Chiang Mai Moat. The Southeast Asian Journal of Tropical Medicine and Public Health, 28, 179–183Google Scholar

  • Okulewicz A., Perec A., Hildebrand J., Okulewicz J. 2003. Calodium hepaticum (Bancroft, 1893) Moravec, 1982 – kapilaria o nietypowym cyklu rozwojowym. Wiadomości Parazytologiczne, 49, 115–124. (In Polish)Google Scholar

  • Resendes A.R., Amaral A.F., Rodrigues A., Almeria S. 2009. Prevalence of Calodium hepaticum (syn. Capillaria hepatica) in house mice (Mus musculus) in the Azores archipelago. Veterinary Parasitology, 160, 340–343. CrossrefWeb of SciencePubMedGoogle Scholar

  • Seong J.K., Huh S., Lee J.S., Oh Y.S. 1995. Helminths in Rattus norvegicus captured in Chunchon, Korea. The Korean Journal of Parasitology, 33:235–237CrossrefPubMedGoogle Scholar

  • Simões R.O., Luque J.L., Faro M.J., Motta E., Maldonad A. 2014. Prevalence of Calodium hepaticum (syn. Capillaria hepatica) in Rattus norvegicus in the urban area of Rio de Janeiro. Revista do Instituto de Medicina Tropical de São Paulo, 56, 455–457. CrossrefWeb of ScienceGoogle Scholar

  • Spratt D.M., Singleton G.R. 2001. Hepatic capillariasis. In: (Eds: W.M., Samuel, M.J. Pybus, A.A. Kocan) Parasitic diseases of wild mammals, 2nd edn. London, Manson Publishing/The Veterinary Press, 365–379Google Scholar

  • Tamaru M., Yamaki S., Jimenez L.A., Sato H. 2015. Morphological and molecular genetic characterization of three Capillaria spp. (Capillaria anatis, Capillaria pudendotecta, and Capillaria madseni) and Baruscapillaria obsignata (Nematoda: Trichuridae: Capillariinae) in avians. Parasitology Research, 114, 4011–4022. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680CrossrefPubMedGoogle Scholar

  • Wright K.A. 1961. Observations on the life cycle of Capillaria hepatica (Bancroft, 1893) with a description of the adult. Canadian Journal of Zoology, 38, 167–182Google Scholar

  • Wysocki E., Nasiłowska M. 1959. Wyniki badań nad helmintofauną szczurów wędrownych. Wiadomości Parazytologiczne, 5, 591–594. (In Polish)Google Scholar

  • Zaleśny G., Hildebrand J., Popiołek M. 2010. Molecular identification of Heterakis spumosa Schneider, 1866 (Nematoda: Ascaridida: Heterakidae) with comparative analysis of its occurrence in two mice species. Annales Zoologici, 60, 647-655. http://dx.doi.org/10.3161/000345410X550517Web of ScienceCrossref

About the article

Received: 2017-04-02

Revised: 2017-05-25

Accepted: 2017-06-30

Published Online: 2017-10-15

Published in Print: 2017-12-20

Citation Information: Acta Parasitologica, Volume 62, Issue 4, Pages 728–732, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0087.

Export Citation

© 2017 W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in