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Acta Parasitologica

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Volume 62, Issue 4


Detection of small number of Giardia in biological materials prepared from stray dogs

Leila Esmailikia
  • Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elahe Ebrahimzade
  • Corresponding author
  • Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Parviz Shayan
  • Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Narges Amininia
  • Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-10-15 | DOI: https://doi.org/10.1515/ap-2017-0088


Giardia lamblia is an intestinal protozoa with intermittent and low shedding especially in dogs, and the detection of Giardia is accompanied with problems such as sampling and diagnostic method. The objective of this study was to detection of Giardia in biological materials with low number of parasite using parasitological and molecular methods, and also to determine whether the examined stray dogs harbor known zoonotic genotype of Giardia. For this aim 85 fecal and duodenal samples were studied from which 1 was positive by Trichrome staining of stool, 4 were positive by staining of duodenal samples. The nested PCR analysis with primers derived from 18 SrRNA showed that the specific PCR product could be amplified in 4 stool and 4 duodenal samples. All positive samples in staining analysis were also positive in nested PCR. No amplification could be observed by nested PCR with primers derived from β giardin gene due to the single copy of gene. Interestingly, the extracted DNA from old fixed stained Giardia positive smears could be also amplified with primers derived from 18SrRNA gene. The sequence analysis of nested PCR products showed that they belong to the genotype D. In conclusion, it is to denote that the Trichrome or Giemsa methods were not suitable for the detection of small number of this parasite in stool and the nested PCR with primers derived from 18S rRNA gene can replace the traditional methods successfully. For detection of Giardia in stool, primers derived from β giardin will not be recommended.

Keywords: Giardia; stray dogs; biological materials


  • Adam R.D. 2001. Biology of Giardia lamblia. Clinical microbiology reviews, 14, 447–475. CrossrefPubMedGoogle Scholar

  • Alves J., Santos A. 2016. Prevalence of Giardia spp. in young dogs using a combination of two diagnostic methods. Acta Parasitologica, 61, 261–266. CrossrefWeb of SciencePubMedGoogle Scholar

  • Babaei Z., Oormazdi H., Akhlaghi L., Rezaie S., Razmjou E., Soltani-Arabshahi S., et al. 2008. Molecular characterization of the Iranian isolates of Giardia lamblia: application of the glutamate dehydrogenase gene. Iranian Journal Public Health, 37, 75–82.Google Scholar

  • Baldursson S., Karanis P. 2011. Waterborne transmission of protozoan parasites: review of worldwide outbreaks–an update 2004–2010. Water Research, 45, 6603–6614. CrossrefGoogle Scholar

  • Ballweber L.R., Xiao L., Bowman D.D., Kahn G., Cama V.A. 2010. Giardiasis in dogs and cats: update on epidemiology and public health significance. Trends in Parasitology, 26, 180–189. CrossrefWeb of SciencePubMedGoogle Scholar

  • Barutzki D., Schaper R. 2003. Endoparasites in dogs and cats in Germany 1999–2002. Parasitology Research, 90, S148-S150. CrossrefWeb of ScienceGoogle Scholar

  • Buret A.G. 2007. Mechanisms of epithelial dysfunction in giardiasis. Gut, 56, 316–317. CrossrefWeb of SciencePubMedGoogle Scholar

  • Cacciò S.M., De Giacomo M., Pozio E. 2002. Sequence analysis of the β-giardin gene and development of a polymerase chain reaction–restriction fragment length polymorphism assay to genotype Giardiaduodenalis cysts from human faecal samples. International Journal for Parasitology, 32, 1023–1030CrossrefGoogle Scholar

  • Cacciò S.M., Ryan U. 2008. Molecular epidemiology of giardiasis. Molecular And Biochemical parasitology, 160, 75–80. CrossrefWeb of SciencePubMedGoogle Scholar

  • De Santis-Kerr A.C., Raghavan M., Glickman N.W., Caldanaro R.J., Moore G.E., Lewis H.B., et al. 2006. Prevalence and risk factors for Giardia and coccidia species of pet cats in 2003–2004. Journal of Feline Medicine & Surgery, 8, 292–301. CrossrefGoogle Scholar

  • Ey P.L., Andrews R.H., Mayrhofer G. 1993. Differentiation of major genotypes of Giardia intestinalis by polymerase chain reaction analysis of a gene encoding a trophozoite surface antigen. Parasitology, 106, 347–356PubMedCrossrefGoogle Scholar

  • Faust E.C., Sawitz W., Tobie J., Odom V., Peres C., Lincicome D.R. 1939. Comparative efficiency of various technics for the diagnosis of protozoa and helminths in feces. The Journal of Parasitology, 25, 241–262CrossrefGoogle Scholar

  • Feng Y., Xiao L. 2011. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clinical Microbiology Reviews, 24, 110–140CrossrefPubMedWeb of ScienceGoogle Scholar

  • Fontanarrosa M.F., Vezzani D., Basabe J., Eiras D.F. 2006. An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): age, gender, breed, mixed infections, and seasonal and spatial patterns. Veterinary Parasitology, 136, 283–295. CrossrefGoogle Scholar

  • Garedaghi Y., Karimi B. 2014. Prevalence of intestinal protozoan parasites in stray dogs of Tabriz city, Iran. Indian of Fundamental and Applied Life Sciences, 4, 20–24Google Scholar

  • Hamnes I.S., Gjerde B.K., Robertson L.J. 2007. A longitudinal study on the occurrence of Cryptosporidium and Giardia in dogs during their first year of life. Acta Veterinaria Scandinavica, 49, 1–10. CrossrefWeb of ScienceGoogle Scholar

  • Hopkins R.M., Meloni B.P., Groth D.M., Wetherall J.D., Reynoldson J.A., Thompson R.A. 1997. Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. The Journal of Parasitology, 44–51PubMedGoogle Scholar

  • Itagaki T., Kinoshita S., Aoki M., Itoh N., Saeki H., Sato N., et al. 2005. Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing. Veterinary Parasitology, 133, 283–287. CrossrefPubMedGoogle Scholar

  • Johansen K., Castro N., Lancaster K., Madrid E., Havas A., Simms J., Sterling C. 2014. Characterization of Giardia lamblia genotypes in dogs from Tucson, Arizona using SSU-rRNA and β-giardin sequences. Parasitology Research, 113, 387–390. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Lalle M., Jimenez-Cardosa E., Cacciò S.M., Pozio E. 2005a. Genotyping of Giardia duodenalis from humans and dogs from Mexico using a β-giardin nested polymerase chain reaction assay. Journal of Parasitology, 91, 203–205. CrossrefGoogle Scholar

  • Lalle M., Pozio E., Capelli G., Bruschi F., Crotti D., Cacciò S.M. 2005b. Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardiaduodenalis and identification of potentially zoonotic subgenotypes. International Journal for Parasitology, 35, 207–213. CrossrefGoogle Scholar

  • Lasek-Nesselquist E., Welch D.M., Sogin M.L. 2010. The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. International Journal for Parasitology, 40, 1063–1074. CrossrefWeb of ScienceGoogle Scholar

  • Mirzaei M. 2010. Prevalence of stray dogs with intestinal protozoan parasites. American Journal of Animal and Veterinary Sciences, 5, 79–83. CrossrefGoogle Scholar

  • Monis P.T., Andrews R.H., Mayrhofer G., Mackrill J., Kulda J., Isaac-Renton J., Ey P.L. 1998. Novel lineages of Giardia intestinalis identified by genetic analysis of organisms isolated from dogs in Australia. Parasitology, 116, 7–19PubMedCrossrefGoogle Scholar

  • Mundim M., Rosa L., Hortencio S., Faria E., Rodrigues R., Cury M. 2007. Prevalence of Giardia duodenalis and Cryptosporidium spp. in dogs from different living conditions in Uberlândia, Brazil. Veterinary Parasitology, 144, 356–359. CrossrefPubMedGoogle Scholar

  • Paz e Silva F.M., Monobe M.M., Lopes R.S., Araujo Jr J. P. 2012. Molecular characterization of Giardia duodenalis in dogs from Brazil. Parasitology Research, 110, 325–334. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Razmi G. 2009. Survey of Dogs’ Parasites in Khorasan Razavi Province, Iran. Iranian Journal of Parasitology, 4, 48–54.Google Scholar

  • Rendtorff R. C. 1979. The experimental transmission of Giardia lamblia among volunteer subjects. Waterborne Transmission of Giardiasis, 45268, 64–81Google Scholar

  • Scaramozzino P., Di Cave D., Berrilli F., D’Orazi C., Spaziani A., Mazzanti S., et al. 2009. A study of the prevalence and genotypes of Giardia duodenalis infecting kennelled dogs. The Veterinary Journal, 182, 231–234. CrossrefGoogle Scholar

  • Shin J.C., Reyes A. W.B., Kim S.-H., Kim S., Park H.-J., Seo K.-W., Song K.-H. 2015. Molecular Detection of Giardia intestinalis from Stray Dogs in Animal Shelters of Gyeongsangbuk-do (Province) and Daejeon, Korea. The Korean Journal of Parasitology, 53, 477. CrossrefPubMedGoogle Scholar

  • Shirani D., Khalili M., Meshgi B. 2006. The Prevalance of Giardia SPP. among the pet dogs of Isfahan. Journal of Veterinary Research, 61, 161–3Google Scholar

  • Shoorijeh S.J., Sadjjadi S.M., Asheri A., Eraghi K. 2008. Giardia spp. and Sarcocystis spp. status in pet dogs of Shiraz, Southern part of Iran. Tropical Biomedicine, 25, 154–159PubMedGoogle Scholar

  • Štrkolcová G., Mad’ar M., Hinney B., Goldová M., Mojžišová J., Halánová M. 2015. Dog’s genotype of Giardia duodenalis in human: first evidence in Europe. Acta Parasitologica, 60, 796–799. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Thompson R.A. 2004. The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Veterinary Parasitology, 126, 15–35. CrossrefPubMedGoogle Scholar

  • Tseng Y., Ho G., Chen T., Huang B., Cheng P.-C., Chen J., Peng S. 2014. Prevalence and genotype of Giardia duodenalis from faecal samples of stray dogs in Hualien city of eastern Taiwan. Tropical Biomedicine, 31, 305–311PubMedGoogle Scholar

  • Van der Giessen J., De Vries A., Roos M., Wielinga P., Kortbeek L., Mank T. 2006. Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates. International Journal for Parasitology, 36, 849–858. CrossrefPubMedGoogle Scholar

  • Volotão A., Costa-Macedo L., Haddad F., Brandao A., Peralta J., Fernandes O. 2007. Genotyping of Giardia duodenalis from human and animal samples from Brazil using β-giardin gene: a phylogenetic analysis. Acta Tropica, 102, 10–19. CrossrefWeb of SciencePubMedGoogle Scholar

  • Yoshiuchi R., Matsubayashi M., Kimata I., Furuya M., Tani H., Sasai K. 2010. Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan. Veterinary Parasitology, 174, 313–316. CrossrefWeb of SciencePubMedGoogle Scholar

About the article

Received: 2016-07-01

Revised: 2017-05-26

Accepted: 2017-07-05

Published Online: 2017-10-15

Published in Print: 2017-12-20

Citation Information: Acta Parasitologica, Volume 62, Issue 4, Pages 733–738, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0088.

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