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Acta Parasitologica

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Volume 62, Issue 4

Issues

Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens

Anju Varghese
  • Corresponding author
  • Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, pookode, Kerala, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Opinder K. Raina / Dinesh Chandra / Bijay R. Mirdha / Naresh H. Kelawala
  • Department of Veterinary Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jayesh B. Solanki
  • Department of Veterinary Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
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  • De Gruyter OnlineGoogle Scholar
/ Niranjan Kumar
  • Department of Veterinary Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Reghu Ravindran
  • Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, pookode, Kerala, India
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  • De Gruyter OnlineGoogle Scholar
/ Anandanarayanan Arun / Ajayta Rialch / Hniang Lalrinkima / Rohan N. Kelawala
  • Department of Veterinary Parasitology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Subhamoy Samanta
Published Online: 2017-10-15 | DOI: https://doi.org/10.1515/ap-2017-0093

Abstract

Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.

Keywords: T.canis; IgG-ELISA; recombinant arginine kinase; cathepsin L-1; TES-26

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About the article

Received: 2017-06-17

Revised: 2017-07-31

Accepted: 2017-08-01

Published Online: 2017-10-15

Published in Print: 2017-12-20


Citation Information: Acta Parasitologica, Volume 62, Issue 4, Pages 775–778, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2017-0093.

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