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Acta Parasitologica

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Volume 63, Issue 1


Contamination of swimming pools and hot tubs biofilms with Acanthamoeba

Behroz Mahdavi Poor
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
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/ Abdolhossein Dalimi
  • Corresponding author
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Email
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/ Fatemeh Ghafarifar
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
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/ Fariba Khoshzaban
  • Department of Parasitology and Mycology, Faculty of Medical Sciences, Shahed University, Tehran, Iran
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/ Jalal Abdolalizadeh
Published Online: 2018-01-17 | DOI: https://doi.org/10.1515/ap-2018-0016


The members of Acanthamoeba genus are ubiquitous amoeba which could be a pathogenic parasite. The amoeba is resistant to the common chlorine concentration that used for disinfecting the swimming pool water. Therefore, the pools can be suitable environments for the survival and multiplication of the amoeba. In this cross sectional study, 10 indoor recreational water centers from different regions of Tabriz city were selected and sampling was done from fixed and floating biofilms of the swimming pools and hot tubs. The samples were cultured and monitored for the presence of amoeba cyst or trophozoite. For molecular identification of Acanthamoeba, PCR (polymerase chain reaction) and sequencing were conducted based on genus specific fragment of 18S ribosomal DNA (Rns). Acanthamoeba contamination was observed in 6 centers of 10 recreational centers. Based on the amoeba isolation from fixed and floating biofilms, 2 (20%) swimming pools, and 5 (50%) hot tubs were contaminated. Based on the type of the sample, the highest contamination was found in the hot tub water (40%) and the least was found in the swimming pools water (10%) and fixed biofilms of the swimming pools (10%). Out of 8 isolates, 5 (62.5%) were shown expected product in PCR amplification. Sequence analysis showed that Acanthamoeba isolates belonged to the T3 and T4 genotypes. The study revealed a high degree of contamination in the indoor recreational water centers in Tabriz city. So, it is essential to pay closer attention to the hygiene of swimming pools and hot tubs.

Keywords: Acanthamoeba; genotype; biofilms; swimming pool; tub


  • Al-Herrawy A., Bahgat N., Mohammed A.E., Ashour A., Hikal W. 2014. Acanthamoeba species in Swimming Pools of Cairo, Egypt. Iranian Journal of Parasitology, 9, 194–201PubMedGoogle Scholar

  • Armand B., Motazedian M.H., Asgari Q. 2016. Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods. Parasitology Research, 115, 63–68. CrossrefWeb of SciencePubMedGoogle Scholar

  • Badirzadeh A., Niyyati M., Z. Babaei H., Amini H., Badirzadeh H., Rezaeian M. 2011. Isolation of free-living amoebae from sarein hot springs in ardebil province, iran. Iranian Journal of Parasitology, 6, 1–8PubMedGoogle Scholar

  • Caumo K., Frasson A.P., Pens C.J., Panatieri L.F., Frazzon A.P., Rott M.B. 2009. Potentially pathogenic Acanthamoeba in swimming pools: a survey in the southern Brazilian city of Porto Alegre. Annals of Tropical Medicine & Parasitology, 103, 477–485. CrossrefWeb of ScienceGoogle Scholar

  • Fabres L.F., Rosa dos Santos S.P., Benitez LB., Rott M.B. 2016. Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil. Acta Parasitologica, 61, 221–227. CrossrefWeb of SciencePubMedGoogle Scholar

  • Fallah E., Mahdavi Pour B., Jamali R., Hatam-Nahavandi K., Asgharzadeh M. 2008. Molecular characterization of Cryptosporidium isolates from cattle in a slaughterhouse in Tabriz, Northwestern Iran. Journal of Biological Sciences, 8, 639–643CrossrefGoogle Scholar

  • Goeres D.M., Loetterle L.R., Hamilton M.A. 2007. A laboratory hot tub model for disinfectant efficacy evaluation. Journal of Microbiological Methods, 68, 184–192. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Hsu B.M., Huang C.C., Chen J.S., Chen N.H., Huang J.T. 2011. Comparison of potentially pathogenic free-living amoeba hosts by Legionella spp. in substrate-associated biofilms and floating biofilms from spring environments. Water Research, 45, 5171–5183. CrossrefWeb of SciencePubMedGoogle Scholar

  • Init I., Lau Y.L., Arin Fadzlun A., Foead A.I., Neilson R.S., Nissapatorn V. 2010. Detection of free living amoebae, Acanthamoeba and Naegleria, in swimming pools, Malaysia. Tropical Biomedicine, 27, 566–577PubMedGoogle Scholar

  • Joslin C.E., Tu E.Y., Shoff M.E., Booton G.C., Fuerst P.A., McMahon T.T., et al. 2007. The association of contact lens solution use and Acanthamoeba keratitis. American Journal of Ophthalmology, 144, 169–180. CrossrefWeb of SciencePubMedGoogle Scholar

  • Kaji Y., Hu B., Kawana K., Oshika T. 2005. Swimming with soft contact lenses: danger of Acanthamoeba keratitis. Lancet Infectious Diseases, 5, 392. CrossrefGoogle Scholar

  • Karamati S.A., Niyyati M., Lorenzo-Morales J., Lasjerdi Z. 2016. Isolation and molecular characterization of Acanthamoeba genotypes isolated from soil sources of public and recreational areas in Iran. Acta Parasitologica, 61, 784–789. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Khan N.A. 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiology Reviews, 30, 564–595. CrossrefPubMedGoogle Scholar

  • Köhsler M., Mrva M., Walochnik J. 2016. Acanthamoeba. In: Walochnik J., Duchêne M (Eds), Molecular Parasitology, Vienna, Springer, pp 285–324Google Scholar

  • Lang G.E., von Heimburg-Elliger A. 1991. [Acanthamoeba keratitis in hard contact lens wearers. Case report and review of the literature of 108 cases]. Klinische Monatsblatter fur Augenheilkunde, 198, 290–294. CrossrefPubMedGoogle Scholar

  • Lasjerdi Z., Niyyati M., Lorenzo-Morales J., Haghighi A., Taghipour N. 2015. Ophthalmology hospital wards contamination to pathogenic free living Amoebae in Iran. Acta Parasitologica, 60, 417–422. CrossrefWeb of SciencePubMedGoogle Scholar

  • Latifi A.R., Niyyati M., Lorenzo-Morales J., Haghighi A., Tabaei S.J., Lasjerdi Z., et al. 2017. Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran. Acta Parasitologica, 62, 104–109. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Mahdavi Poor B., Rashedi J., Asgharzadeh M., Fallah E., Hatam-Nahavandi K., Dalimi A. 2015. Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran. International Journal of Molecular and Cellular Medicine, 4, 235–239PubMedGoogle Scholar

  • Marciano-Cabral F., Cabral G. 2003. Acanthamoeba spp. as agents of disease in humans. Clinical Microbiology Reviews, 16, 273–307PubMedCrossrefGoogle Scholar

  • Mirjalali H., Niyyati M., Abedkhojasteh H., Babaei Z., Sharifdini M., Rezaeian M. 2013. Pathogenic assays of Acanthamoeba belonging to the T4 genotype. Iranian Journal of Parasitology, 8, 530–535PubMedGoogle Scholar

  • Nahavandi K.H., Fallah E., Jamali R. 2008. Genetic characterization of Giardia intestinalis strains from patients having sporadic giardiasis by using PCR assay. Journal of Medical Sciences, 8, 310–315CrossrefGoogle Scholar

  • Niyyati M., Lasgerdi Z., Lorenzo-Morales J. 2015a. Detection and molecular characterization of potentially pathogenic free-living amoebae from water sources in Kish Island, Southern Iran. Microbiology insights, 8, 1–6. CrossrefGoogle Scholar

  • Niyyati M., Nazar M., Haghighi A., Nazemalhosseini E. 2015b. Reporting of T4 genotype of Acanthamoeba isolates in recreational water sources of Gilan province, northern Iran. Novelty in Biomedicine, 3, 20–24.Google Scholar

  • Niyyati M., Saberi R., Lorenzo-Morales J., Salehi R. 2016. High occurrence of potentially-pathogenic free-living amoebae in tap water and recreational water sources in South-West Iran. Tropical Biomedicine, 33, 95–101Google Scholar

  • Ondriska F., Mrva M., Lichvár M., Ziak P., Murgasova Z., Nohýnková E. 2003. First cases of Acanthamoeba keratitis in Slovakia. Annals of Agricultural and Environmental Medicine, 11, 335–341Google Scholar

  • Page F.C. 1988. A new key to freshwater and soil gymnamoebae: with instructions for culture. Ambleside, Cumbria, England, pp 92–96.Google Scholar

  • Schuster F.L., Visvesvara G.S. 2004. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. International Journal for Parasitology, 34, 1001–1027. .CrossrefPubMedGoogle Scholar

  • Shokri A., Sarvi S., Daryani A., Sharif M. 2016. Isolation and Genotyping of Acanthamoeba spp. as Neglected Parasites in North of Iran. The Korean Journal of Parasitology, 54, 447–453. .CrossrefPubMedGoogle Scholar

  • Siddiqui R., Khan N.A. 2012. Biology and pathogenesis of Acanthamoeba. Parasites and Vectors, 5, 262. .CrossrefGoogle Scholar

  • Rasti S., Assadi M.A., Iranshahi L., Saffari M., Gilasi H.R., Pourbabaee M. 2012. Assessment of microbial contamination and physicochemical condition of public swimming pools in Kashan, Iran. Jundishapur Journal of Microbiology, 5, 450–455. CrossrefWeb of ScienceGoogle Scholar

  • Solgi R., Niyyati M., Haghighi A., Taghipour N., Tabaei S.J., Eftekhar M., et al. 2012. Thermotolerant Acanthamoeba spp. isolated from therapeutic hot springs in northwestern Iran. Journal of Water and Health, 10, 650–656. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Sukthana Y., Lekkla A., Sutthikornchai C., Wanapongse P., Vejjajiva A., Bovornkitti S. 2005. Spa, springs and safety. Southeast Asian Journal of Tropical Medicine & Public Health, 36, 10–16Google Scholar

  • Tung M.C., Hsu B.M., Tao C.W., Lin W.C., Tsai H.F., Ji D.D., et al. 2013. Identification and significance of Naegleria fowleri isolated from the hot spring which related to the first primary amebic meningoencephalitis (PAM) patient in Taiwan. International Journal for Parasitology, 43, 691–696. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Zanella J., Costa SOPD., Zacaria J., Echeverrigaray S. 2012. A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples. Brazilian Archives of Biology and Technology, 55, 01–06. CrossrefWeb of ScienceGoogle Scholar

About the article

Received: 2017-09-08

Revised: 2017-11-05

Accepted: 2017-11-08

Published Online: 2018-01-17

Published in Print: 2018-03-26

Conflict of interest: The authors declare that there is no conflict of interest.

Citation Information: Acta Parasitologica, Volume 63, Issue 1, Pages 147–153, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0016.

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