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Acta Parasitologica

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Volume 63, Issue 1

Issues

Contamination of swimming pools and hot tubs biofilms with Acanthamoeba

Behroz Mahdavi Poor
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Other articles by this author:
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/ Abdolhossein Dalimi
  • Corresponding author
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Email
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/ Fatemeh Ghafarifar
  • Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
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/ Fariba Khoshzaban
  • Department of Parasitology and Mycology, Faculty of Medical Sciences, Shahed University, Tehran, Iran
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/ Jalal Abdolalizadeh
Published Online: 2018-01-17 | DOI: https://doi.org/10.1515/ap-2018-0016

Abstract

The members of Acanthamoeba genus are ubiquitous amoeba which could be a pathogenic parasite. The amoeba is resistant to the common chlorine concentration that used for disinfecting the swimming pool water. Therefore, the pools can be suitable environments for the survival and multiplication of the amoeba. In this cross sectional study, 10 indoor recreational water centers from different regions of Tabriz city were selected and sampling was done from fixed and floating biofilms of the swimming pools and hot tubs. The samples were cultured and monitored for the presence of amoeba cyst or trophozoite. For molecular identification of Acanthamoeba, PCR (polymerase chain reaction) and sequencing were conducted based on genus specific fragment of 18S ribosomal DNA (Rns). Acanthamoeba contamination was observed in 6 centers of 10 recreational centers. Based on the amoeba isolation from fixed and floating biofilms, 2 (20%) swimming pools, and 5 (50%) hot tubs were contaminated. Based on the type of the sample, the highest contamination was found in the hot tub water (40%) and the least was found in the swimming pools water (10%) and fixed biofilms of the swimming pools (10%). Out of 8 isolates, 5 (62.5%) were shown expected product in PCR amplification. Sequence analysis showed that Acanthamoeba isolates belonged to the T3 and T4 genotypes. The study revealed a high degree of contamination in the indoor recreational water centers in Tabriz city. So, it is essential to pay closer attention to the hygiene of swimming pools and hot tubs.

Keywords: Acanthamoeba; genotype; biofilms; swimming pool; tub

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About the article

Received: 2017-09-08

Revised: 2017-11-05

Accepted: 2017-11-08

Published Online: 2018-01-17

Published in Print: 2018-03-26


Conflict of interest: The authors declare that there is no conflict of interest.


Citation Information: Acta Parasitologica, Volume 63, Issue 1, Pages 147–153, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0016.

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