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Acta Parasitologica

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Volume 63, Issue 1

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Morphological and molecular characterization of Seuratascaris numidica (Seurat, 1917) (Ascaridida: Ascarididae)

Hui-Xia Chen
  • Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, P. R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kuang Zhang
  • Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, P. R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lu-Ping Zhang
  • Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, P. R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Liang Li
  • Corresponding author
  • Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, P. R. China
  • Email
  • Other articles by this author:
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Published Online: 2018-01-17 | DOI: https://doi.org/10.1515/ap-2018-0017

Abstract

Seuratascaris numidica (Seurat, 1917) is a specialized nematode species parasitizing amphibians only. In the present study, the detailed morphology of this poorly known species was studied using light and scanning electron microscopy based on the newly material collected from Hoplobatrachus chinensis (Osbeck) (Amphibia: Anura) in China. We found that the relative length of intestinal caecum in our male specimens (representing 68.4–71.1% of oesophageal length) is slighter longer than the previously reported data (not over 60.0% of oesophageal length). Our SEM observations also revealed the presence of ca. 64–76 small conical denticles on each lip. In addition, Angusticaecum wuyiensis Wang, 1981, collected from Rana schmackeri Boettger (Amphibia: Anura) from Wuyi Mountain in Fujian Province, China was considered as a new synonym of S. numidica. The ITS and cox1 sequences of S. numidica were also sequenced for the first time and there is no nucleotide variability detected in both regions. The present supplementary morphological and molecular data (especially the ITS and cox1 sequences) obtained herein is extremely important and useful to determine the morphological variability, population genetics and phylogenetic position of S. numidica in the future.

Keywords: Nematode; Amphibia; Seuratascaris; morphology; internal transcribed spacer (ITS); cytochrome c oxidase subunit 1 (cox1)

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About the article

Received: 2017-10-27

Revised: 2017-11-06

Accepted: 2017-11-08

Published Online: 2018-01-17

Published in Print: 2018-03-26


Citation Information: Acta Parasitologica, Volume 63, Issue 1, Pages 154–159, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0017.

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