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Acta Parasitologica

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Volume 63, Issue 2

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Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782)

Bo Zhang
  • Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China
  • Other articles by this author:
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/ Yanhua Zhai
  • Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China
  • Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, People’s Republic of China
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/ Zemao Gu
  • Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China
  • Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, People’s Republic of China
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  • De Gruyter OnlineGoogle Scholar
/ Yang Liu
  • Corresponding author
  • Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China
  • Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, People’s Republic of China
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Published Online: 2018-04-13 | DOI: https://doi.org/10.1515/ap-2018-0026

Abstract

A Myxobolus species and a Thelohanellus species infecting Carassius auratus gibelio (Bloch, 1782) were redescribed by their morphological, histological and molecular characterization. In the present study, the Myxobolus species infecting the muscle was identified as Myxobolus kingchowensis Chen et Ma, 1998 by the morphological and molecular data. Histologically, mature spores of M. kingchowensis were observed in the intercellular and connective tissue of muscle, though the plasmodia were not found. In addition, scattered spores also occurred in the intercellular of haematopoietic cells, intraepithelial of the renal tubules and interior of the melano-macrophage centres. Phylogenetic analysis showed that M. kingchowensis clustered in the clade of muscle-infecting Myxobolus species, further supporting muscle as the infection site of M. kingchowensis. The present Thelohanellus species infecting the gills was identified conspecific as Thelohanellus sinensis reported in Sun (2006) (mark it as T. sinensis-Sun)based on spore morphology, biological traits (host specificity and organ specificity), and molecular data. However, compared with the original description of T. sinensis Chen et Hsieh, 1960, the present Thelohanellus species and T. sinensis-Sun both infecting the gills of gibel carp are distinguishable from the original description in the host and infection site, which made the validity of T. sinensis-Sun dubious. Due to the absence of molecular data in the original description of T. sinensis, we suggest marking the present species and T. sinensis-Sun as T. cf. sinensis to avoid the confusion until T. sinensis is obtained from the type host and type infection site.

Keywords: Myxosporea; Myxobolus kingchowensis; Thelohanellus sinensis; Carassius auratus gibelio; China

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About the article

Received: 2017-11-13

Revised: 2018-01-11

Accepted: 2018-01-12

Published Online: 2018-04-13

Published in Print: 2018-06-26


Citation Information: Acta Parasitologica, Volume 63, Issue 2, Pages 221–231, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0026.

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