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Acta Parasitologica

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Volume 63, Issue 2

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Structural predication and antigenic analysis of Toxoplasma gondii ROP20

Jian Zhou
  • Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China
  • Department of Sports Medicine Research Center, Central South University, Changsha, Hunan 410008 People’s Republic of China
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/ Wanchun Wang
  • Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China
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/ Pengxia Song
  • Department of Medicine, Quzhou College of Technology, Quzhou, Zhejiang 324000, People’s Republic of China
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/ Lin Wang
  • Department of Electroneurophysiology, Jinan Children’s Hospital, Jinan, Shandong 250022, People’s Republic of China
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/ Yali Han
  • Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong 250012, People’s Republic of China
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/ Jingjing Guo
  • Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong 250012, People’s Republic of China
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/ Zhen Hao
  • Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong 250012, People’s Republic of China
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/ Xi Zhu
  • Department of Lymphoma and Hematology, Hunan Cancer Hospital, Changsha, Hunan 410000, People’s Republic of China
  • University of South China, Hengyang, Hunan 421001, People’s Republic of China
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/ Qiong Zhou
  • Department of Cardiology, The Fourth Hospital of Changsha, Changsha, Hunan 410006, People’s Republic of China
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/ Xiadong Du
  • Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China
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/ Gang Lu
  • Department of Pathology, Taishan Medical College, Taian, Shandong 271000, People’s Republic of China
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/ Shenyi He
  • Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong 250012, People’s Republic of China
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/ Yingquan Luo
  • Corresponding author
  • Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China
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Published Online: 2018-04-13 | DOI: https://doi.org/10.1515/ap-2018-0028

Abstract

Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii. In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online procedure and Visual Molecular Dynamics (VMD) software. The structure analysis fully indicated that ROP20 protein is an important member of the ROP family. Furthermore, We used DNASTAR software and Epitope Database online service to analyze liner-B cell epitopes and T-cell epitopes of ROP20 protein. All the analysis results of ROP20 protein can provide positive information on treatment and vaccine for toxoplasmosis. Moreover, ROP20 gene was obtained from PCR, and a recombinant eukaryotic expression vector (pEGFP-C1-ROP20) was constructed in the following study. After restriction enzyme digestion, the constructed plasmid was transfected into HEK 293-T cells. The RT-PCR result indicated that the recombinant plasmid could transcribe successfully in HEK 293-T cell. The results of western blotting indicated the expressed proteins can be recognized by anti-STAg mouse sera.

Keywords: Toxoplasma gondii; ROP20; secondary structures; constructed plasmids; expression

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About the article

Received: 2017-11-27

Revised: 2018-01-12

Accepted: 2018-01-18

Published Online: 2018-04-13

Published in Print: 2018-06-26


Conflict of interestConflict of interests: The authors wish to declare that there is no known conflict of interests associated with this publication.


Citation Information: Acta Parasitologica, Volume 63, Issue 2, Pages 244–251, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0028.

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