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Acta Parasitologica

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Volume 63, Issue 2

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Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters

Fabiola del Rocío Villalobos-Gómez
  • Universidad Autónoma de Aguascalientes, Departamento de Morfología, Centro de Ciencias Básicas, Edificio 202, Av. Universidad 940 Ciudad Universitaria C.P. 20130, Aguascalientes, Ags., México
  • Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, CDMX, México
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/ Mario García-Lorenzana
  • Área de Neurociencias, Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Vicentina, Iztapalapa, C.P. 09340 CDMX, México
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/ Galileo Escobedo
  • Universidad Nacional Autónoma de México, Hospital General de México “Dr. Eduardo Liceaga”, Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina, Dr. Balmis #148, Col. Doctores, C.P. 06720, Ciudad de México, México
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/ Patricia Talamás-Rohana
  • Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, Avenida Instituto Politécnico Nacional 2508, C.P. 07360, Ciudad de México, México
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/ Rogelio Salinas-Gutiérrez
  • Universidad Autónoma de Aguascalientes, Departamento de Estadística, Centro de Ciencias Básicas, Edificio 202, Av. Universidad 940 Ciudad Universitaria C.P. 20130, Aguascalientes, Ags., México
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/ Verónica-Ivonne Hernández-Ramírez
  • Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, Avenida Instituto Politécnico Nacional 2508, C.P. 07360, Ciudad de México, México
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/ Esperanza Sánchez-Alemán
  • Universidad Autónoma de Aguascalientes, Departamento de Morfología, Centro de Ciencias Básicas, Edificio 202, Av. Universidad 940 Ciudad Universitaria C.P. 20130, Aguascalientes, Ags., México
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/ María del Rosario Campos-Esparza
  • Universidad Autónoma de Aguascalientes, Departamento de Morfología, Centro de Ciencias Básicas, Edificio 202, Av. Universidad 940 Ciudad Universitaria C.P. 20130, Aguascalientes, Ags., México
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/ Martín Humberto Muñoz-Ortega
  • Departamento de Química, Universidad Autónoma de Aguascalientes. Av. Universidad # 940, Ciudad Universitaria, C. P. 20131, Aguascalientes, Ags., México
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/ Javier Ventura-Juárez
  • Corresponding author
  • Universidad Autónoma de Aguascalientes, Departamento de Morfología, Centro de Ciencias Básicas, Edificio 202, Av. Universidad 940 Ciudad Universitaria C.P. 20130, Aguascalientes, Ags., México
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Published Online: 2018-04-13 | DOI: https://doi.org/10.1515/ap-2018-0031

Abstract

The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1β, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1β, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.

Keywords: L220 Entamoeba histolytica; neutrophils; macrophages; epinephrine; esmolol; nicotine; vecuronium bromide; cytokines

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About the article


Received: 2017-07-04

Revised: 2017-12-13

Accepted: 2018-01-17

Published Online: 2018-04-13

Published in Print: 2018-06-26


Conflict of interestConflict of interest statement: The authors have not interest conflict


Citation Information: Acta Parasitologica, Volume 63, Issue 2, Pages 270–279, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0031.

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