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Acta Parasitologica

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Volume 63, Issue 3

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First molecular identification of an agent of diplostomiasis, Diplostomum pseudospathaceum (Niewiadomska 1984) in the United Kingdom and its genetic relationship with populations in Europe

Egie Elisha Enabulele
  • Corresponding author
  • Department of Animal and Environmental Biology, Faculty of Life Sciences, University of Benin Nigeria, PMB 1154, Benin City, Africa
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Agnes Ogheneruemu Awharitoma
  • Department of Animal and Environmental Biology, Faculty of Life Sciences, University of Benin Nigeria, PMB 1154, Benin City, Africa
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Scott P. Lawton
  • Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston upon Thames, Surrey, KT1 2EE, UK
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ruth S. Kirk
  • Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston upon Thames, Surrey, KT1 2EE, UK
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-07-04 | DOI: https://doi.org/10.1515/ap-2018-0054

Abstract

Trematode genus Diplostomum comprises of parasitic species which cause diplostomiasis, the ‘white eye’ disease in fish and heavy infection can result in mortality. The increasing availability of DNA sequences of accurately identified Diplostomum species on public data base presently enables the rapid identification of species from novel sequences. We report the first molecular evidence of the occurrence of D. pseudospathaceum in the United Kingdom. Two gene regions, nuclear internal transcribed spacer cluster (ITS1-5.8S-ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1) of cercariae from infected aquatic snails, Lymnaea stagnalis collected in several locations in Southern England were sequenced. Phylogenetic analysis based on both sequenced genes revealed that the novel sequences were D. pseudospathaceum. Molecular diversity analysis of published D. pseudospathaceum cox1 sequences from seven countries in Europe and the novel sequences from the present study revealed high diversity, but low nucleotide divergence and a lack of gene differentiation between the populations. Haplotype network analysis depicted a star-like pattern and revealed a lack of geographic structure in the population. Fixation indices confirmed gene flow between populations and we suspect high levels of dispersal facilitated by highly mobile second intermediate (fish) and definitive (piscivorous birds) host may be driving gene flow between populations. Neutrality tests and mismatch distribution indicated recent population growth/expansion for D. pseudospathaceum in Europe.

Keywords: Diplostomum pseudospathaceum; Lymnaea stagnalis; United Kingdom; Europe; molecular diversity; population genetics

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About the article

Received: 2017-10-30

Revised: 2018-02-20

Accepted: 2018-03-02

Published Online: 2018-07-04

Published in Print: 2018-09-25


Citation Information: Acta Parasitologica, Volume 63, Issue 3, Pages 444–453, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0054.

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