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Acta Parasitologica

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Volume 63, Issue 3

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Molecular characterization of Theileria spp. in livestock and the first report on the occurrence of Theileria sp. OT3 in Iran

Seyyed Javad Seyyed Tabaei
  • Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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/ Adel Spotin
  • Corresponding author
  • Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  • Email
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  • De Gruyter OnlineGoogle Scholar
/ Ramin Pouriran / Abbas Shahbazi
  • Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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/ Amirreza Javadi Mamaghani
  • Corresponding author
  • Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-07-04 | DOI: https://doi.org/10.1515/ap-2018-0061

Abstract

Theileria lestoquardi, T. ovis, and T. annulata are recognized as major causative agents for ovine and cattle theileriosis in Iran, respectively. Recently, there have been reports on the presence of Theileria spp. (Theileria sp. OT1, Theileria sp. OT3, and Theileria sp.). In this study, 37 blood samples were collected from sheep and cattle with clinically suspected Theileria infection in the Northwest of Iran. The samples were analyzed using a light microscope. DNA samples were amplified via nested-polymerase chain reaction (PCR) of 18S rRNA gene. The amplicons were digested with HpaII, following restriction fragment length polymorphism (RFLP) and sequenced to reconfirm Theileria species. The microscopic examination indicated that 4 out of 37 (10.8%) blood samples were infected with Theileria spp. Based on the nested PCR-RFLP and sequencing data, 5.4%, 13.5%, and 27% of blood samples were infected with Theileria sp. OT3, T. ovis, and T. annulata, respectively. The pairwise distance matrix of Theileria sp. OT3 showed 99.8–100% identity and 0–0.2% divergence in comparison with the registered sequences. The present study is the first report of Theileria sp. OT3 in Iran. To the evaluate evolution of Theileria spp. and providing resultant genetic data, further research with a larger sample is necessary in the region.

Keywords: Theileria sp. OT3; Theileria infection; sequencing; sheep; Iran

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About the article

a.javadi@sbmu.ac.ir

† Adel Spotin and Amirreza Javadi Mamaghani contributed equally to this work


Received: 2017-07-13

Revised: 2018-01-31

Accepted: 2018-04-11

Published Online: 2018-07-04

Published in Print: 2018-09-25


Conflict of interest: None declared


Citation Information: Acta Parasitologica, Volume 63, Issue 3, Pages 515–521, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0061.

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