Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Acta Parasitologica

IMPACT FACTOR 2017: 1.039
5-year IMPACT FACTOR: 1.121

CiteScore 2017: 1.17

SCImago Journal Rank (SJR) 2017: 0.641
Source Normalized Impact per Paper (SNIP) 2017: 0.738

More options …
Volume 63, Issue 3


Molecular phylogenetic position of Haplometroides intercaecalis (Digenea, Plagiorchiidae)

Maria Isabel Müller
  • Corresponding author
  • Universidade Estadual Paulista “Júlio de Mesquita Filho”, Instituto de Biociências, Departamento de Parasitologia, Distrito de Rubião Júnior s/n, CEP 18618-689, Botucatu, São Paulo, Brazil
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Drausio Honorio Morais
  • Universidade Federal Rural da Amazônia (UFRA), PA 275, Km 13, Zona Rural. CEP: 68.515-000, Parauapebas, Pará, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Reinaldo José da Silva
  • Universidade Estadual Paulista “Júlio de Mesquita Filho”, Instituto de Biociências, Departamento de Parasitologia, Distrito de Rubião Júnior s/n, CEP 18618-689, Botucatu, São Paulo, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-07-04 | DOI: https://doi.org/10.1515/ap-2018-0062


Three valid species of Haplometroides Odhner, 1910 parasitise snakes and amphisbaenians from South America. This study provides additional data on morphometric and molecular phylogenetic position inferred from the nuclear ribosomal gene 28S (partial). DNA sequences were isolated from Haplometroides intercaecalis Silva, Ferreira and Strüssmann, 2007 found in one specimen of Phalotris matogrossensis Lema, D’Agostini and Cappellari, 2005. Five digenean specimens were recovered from the esophagus of this snake, and four specimens were used for morphometrical studies and one specimen for molecular analysis. Phylogenetic analysis using maximum likelihood and Bayesian methods was conducted with sequences available for the order Plagiorchiida and its phylogenetic position places H. intercaecalis among the brachycoeliids Brachycoelium (Dujardin, 1845) Stiles and Hassall, 1898 and Parabrachycoelium Pérez-Ponce de León, Mendoza-Garfias, Razo-Mendivil and Parra-Olea, 2011, and the mesocoeliid Mesocoelium Odhner, 1910, not closely related to plagiorchids as expected. Due to morphological differences among these families, it may be necessary to create a new family to accommodate Haplometroides spp. However, more genera/taxa as well as other molecular markers should be added in future studies to confirm our results and resolve this matter. This is the first phylogenetic positioning of digeneans of the genus Haplometroides, contributing to the systematic analysis of the helminthological biodiversity of Neotropical snakes.

Keywords: Phalotris matogrossensis; 28S rDNA gene; parasite; neotropical snakes


  • Brooks D.R., O’Grady R.T., Glen D.R. 1985. Phylogenetic analysis of the Digenea (Platyhelminthes: Cercomeria) with comments on their adaptive radiation. Canadian Journal of Zoology, 63, 411–443. CrossrefGoogle Scholar

  • Brooks D.R., Bandoni S.M., MacDonald C.M., O’Grady R.T. 1989. Aspects of the phylogeny of the Trematoda Rudolphi, 1808 (Platyhelminthes: Cercomeria). Canadian Journal of Zoology, 67, 2609–2624. CrossrefGoogle Scholar

  • Edgar R.C. 2004. Muscle: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics, 5,113. CrossrefPubMedGoogle Scholar

  • Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hosdijk W., Gascuel O. 2010. New algorithms and methods to estimate Maximum-Likelihood Phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., et al. 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120PubMedCrossrefGoogle Scholar

  • Lema T., DÁgostini M.F., Cappellari L.H. 2005. Nova espécie de Phalotris, redescrição de P. tricolor e osteologia craniana (Serpentes, Elapomorphinae). Iheringia, 95, 65–78CrossrefGoogle Scholar

  • Maldonado J.A., Loker E.S., Morgan J.A.T., Rey L., Lanfredi R.M. 2001. Description of the adult worms of a new Brazilian isolate of Echinostoma paraensis (Platyhelminthes: Digenea) from its natural vertebrate host Nectomys squamipes by light and scanning electron microscopy and molecular analysis. Parasitology Research, 87, 840–848CrossrefGoogle Scholar

  • Mendoza-Palmero C.A., Blasco-Costa I., Scholz T. 2015. Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasites and Vectors, 8, 164. CrossrefGoogle Scholar

  • Pérez-Ponce de León G., Mendoza-Garfias B., Razo-Mendivil U., Parra-Olea G. 2011. A new genus and species of Brachycoeliidae (Digenea) from Chiropterontriton sp. (Caudata: Plethodontidae) in Mexico and its phylogenetic position within the Plagiorchiida based on partial sequences of the 28S ribosomal RNA gene. Journal of Parasitology, 97, 128–134. CrossrefGoogle Scholar

  • Posada D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253–1256. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Rambaut A. 2009. Molecular evolution, phylogenetics and epidemiology: Fig-Tree. Available at http//tree.bio.ed.ac.uk/software/figtree/ (accessed 10 November 2015)

  • Razo-Mendivil, U., Pérez-Ponce de Léon, G. 2011. Testing the evolutionary and biogeographical history of Glyphtelmins (Digenea: Plagiorchiida) a parasite of anurans, through a simultaneous analysis of molecular and morphological data. Molecular Phylogenetics and Evolution, 59, 331–341. CrossrefPubMedGoogle Scholar

  • Ronquist F., Huelsenbeck J. P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574CrossrefPubMedGoogle Scholar

  • Santos K.R., Barrella T.H., Zica E.O.P., Silva R.J. 2008. New report on parasitism by Haplometroides buccicola (Digenea, Plagiorchiidae) in brazilian snakes. Journal of Venomous Animals Toxins including Tropical Diseases, 14, 527–532CrossrefGoogle Scholar

  • Silva R.J., Barrella T.H. 2002. Micrurus frontalis as a new host recorded for Haplometroides odhneri (Trematoda, Digenea, Plagiorchiidae). Revista Brasileira de Parasitologia Veterinaria, 11, 47–8Google Scholar

  • Silva R.J., Andrade P.A., Monteiro e Silva H.A., Rossellini M., Barrella T.H. 2005a. Report on the occurrence of Haplometroides buccicola (Trematoda, Digenea, Plagiorchiidae) infecting Phalotris lativittatus (Serpentes, Colubridae) in Brazil. Journal of Venomous Animals Toxins including Tropical Diseases, 11, 373–9CrossrefGoogle Scholar

  • Silva R.J., Zica E.O.P., Cruz M., O’Reilly J.C., Costa M.C. 2005b. Occurrence of Haplometroides odhneri (Trematoda, Digenea, Plagiorchiidae) infecting Leptotyphlops koppesi (Serpentes, Leptotyphlopidae). Arquivos Brasileiros de Medicina Veterinaria e Zootecnia, 57, 267–269CrossrefGoogle Scholar

  • Silva R.J., Ferreira V.L., Strüssmann C. 2007. New species of Haplometroides (Digenea, Plagiorchiidae) from Phalotris nasutus (Gomes, 1915) (Serpentes, Colubridae). Journal of Parasitology, 93, 917–921CrossrefGoogle Scholar

  • Silva R.J., Béda A.F., Ferreira V.L. 2008. New record of Haplometroides intercaecalis (Digenea, Plagiorchiidae) infecting a Brazilian snake. Journal of Venomous Animals Toxins including Tropical Diseases, 14, 161–165Web of ScienceCrossrefGoogle Scholar

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 12, 2725–2729Web of ScienceGoogle Scholar

  • Tkach V., Pawlowski J., Mariaux J. 2000. Phylogenetic analysis of the suborder plagiorchiata (Platyhelminthes, Digenea) based on partial lsrDNA sequences. International Journal for Parasitology, 30, 83–93PubMedGoogle Scholar

  • Tkach V.V., Grabda-Kazuska B., Swiderski Z. 2001. Systematic position and phylogenetic relationships of the family Omphalometridae (Digenea, Plagiorchiida) inferred from partial lsrDNA sequences. International Journal for Parasitology, 31, 81–85CrossrefPubMedGoogle Scholar

  • Tkach V.V. 2008. Family Plagiorchiidae Lühe, 1901. In: (Eds: R.A. Bray, D. Gibson, A. Jones). Keys to the Trematoda. CAB International, Wallingford, Oxfordshire, U.K. Vol. 3, pp. 824Google Scholar

  • Vidal V., Ortiz J., Diaz J.I., Zafrilla B., Bonete M.J., Ruiz de Ybañez M.R. et al. 2015. Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson,1942). Journal of Helminthology, 1–9. CrossrefWeb of ScienceGoogle Scholar

  • Xia X. 2013. DAMBE 5. A comprehensive software package for data analysis in molecular biology and evolution. Molecular Biology and Evolution, 30, 1720-1728. CrossrefPubMedGoogle Scholar

About the article

Received: 2018-03-05

Revised: 2018-04-10

Accepted: 2018-04-12

Published Online: 2018-07-04

Published in Print: 2018-09-25

Citation Information: Acta Parasitologica, Volume 63, Issue 3, Pages 522–526, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0062.

Export Citation

© 2018 W. Stefański Institute of Parasitology, PAS.Get Permission

Comments (0)

Please log in or register to comment.
Log in