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Acta Parasitologica

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Volume 63, Issue 3

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Screening of Cercopithifilaria bainae and Hepatozoon canis in ticks collected from dogs of Northeastern Brazil

Marcos Antônio Bezerra Santos / Lucia Oliveira de Macedo / Domenico Otranto / Carlos Alberto do Nascimento Ramos
  • Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ana Gabriela de Oliveira do Rêgo
  • Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alessio Giannelli / Leucio Câmara Alves
  • Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, Brazil
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gílcia Aparecida de Carvalho / Rafael Antonio Nascimento Ramos
Published Online: 2018-07-04 | DOI: https://doi.org/10.1515/ap-2018-0069

Abstract

Rhipicephalus sanguineus sensu lato (s.l.) is one of the most widespread ixodid ticks and is a competent vector of several vector-borne pathogens of veterinary and medical concern. For instance, this tick species transmits nematodes of the genus Cercopithifilaria and protozoa of the genus Hepatozoon to carnivores, including dogs. Here we investigated the occurrence of Cercopithifilaria spp. and Hepatozoon spp. in a population of ticks collected from naturally infested dogs living in rural areas of Northeastern Brazil. From August 2016 to June 2017, 758 tick specimens (mean ticks per month = 68.9 ± 71.4) were sampled from 75 dogs (mean ticks per dog = 10.11 ± 5.2) and dissected under a stereomicroscope in order to visualize Cercopithifilaria spp. larvae and Hepatozoon spp. oocysts and sporocysts. R. sanguineus s.l. was the only species collected, peaking in September (n = 273) and decreasing in February 2017 (n = 39). Different larval stages of Cercopithifilaria bainae were identified in 7 out of 758 (0.93%) ticks. In addition, 4 specimens (0.53%) were positive for oocysts and free sporocysts of Hepatozoon canis. The identity of both species of parasites was molecularly confirmed. These results account for the predominance of R. sanguineus (s.l.) in domestic dogs from rural locations of the study area, as well as for the presence C. bainae and H. canis in these tick populations.

Keywords: Protozoa; filarial; dogs; tick; animal health

References

  • Aktas M., Özübek S. 2017. Transstadial transmission of Hepatozoon canis by Rhipicephalus sanguineus (Acari: Ixodidae) in field conditions. Journal of Medical Entomology, 4, 1044–1048. CrossrefWeb of ScienceGoogle Scholar

  • Almeida G.L.G., Vicente, J.J. 1984. Cercopithifilaria bainae sp. n. parasita de Canis familiaris (L.) (Nematoda, Filarioidea). Atas da Sociedade Biologica do Rio de Janeiro, 24, 18.Google Scholar

  • Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410. CrossrefPubMedGoogle Scholar

  • Andersson M.O., Chitimia-Dobler L. 2017. Detection of Cercopithifilaria bainae in western Romania. Parasitology Research, 116, 3235–3238. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Ayres M., Ayres M.J., Ayres D.L., Santos A.A.S. 2007. BioEstat 5.0: aplicações estatísticas nas áreas das ciências biológicas e médicas. Sociedade Civil Mamirauá, pp. 364. (In Spanish)Google Scholar

  • Baneth G. 2011. Perspectives on canine and feline hepatozoonosis. Veterinary Parasitology. 181, 3–11. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Baneth G., Samish M., Shkap V. 2007. Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). Journal of Parasitology, 93, 283–299. CrossrefWeb of ScienceGoogle Scholar

  • Brianti E., Otranto D., Dantas-Torres F., Weigl S., Latrofa M.S., Gaglio G., et al. 2012. Rhipicephalus sanguineus (Ixodida, Ixodidae) as intermediate host of a canine neglected filarial species with dermal microfilariae. Veterinary Parasitology, 183, 330–337. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Cunha N.C., Fonseca A.H., Rezende J., Rozental T., Favacho A.R.M., Barreira J.D., et al. 2009. First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro. Pesquisa Veterinaria Brasileira, 29, 105–108CrossrefWeb of ScienceGoogle Scholar

  • Dantas – Torres F., Figueiredo L.A., Otranto D. 2011. Seasonal variation in the effect of climate on the biology of Rhipicephalus sanguineus in southern Europe. Parasitology, 138, 527–536. CrossrefWeb of SciencePubMedGoogle Scholar

  • Dantas-Torres F. 2010. Biology and ecology of the brown dog tick, Rhipicephalus sanguineus. Parasites and Vectors, 3, 26. CrossrefGoogle Scholar

  • Dantas-Torres F., Figueiredo L.A., Brandão-Filho S.P. 2006. Rhipicephalus sanguineus (Acari: Ixodidade), the brown dog tick, parasitizing humans in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 39, 64–67. CrossrefGoogle Scholar

  • Demoner L.C., Rubini A.S., Paduan K.S., Metzger B., Antunes J.M.A.P., Martins T.F., et al. 2013. Investigation of tick vectors of Hepatozoon canis in Brazil. Ticks and Tick Borne Diseases, 4, 542–546. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Forlano M., Scofield A., Elisei C., Fernandes K.R., Ewing S.A., Massard C.L. 2005. Diagnosis of Hepatozoon spp. in Amblyomma ovale and its experimental transmission in domestic dogs in Brazil. Veterinary Parasitology, 134, 1–7. CrossrefPubMedGoogle Scholar

  • Gabrielli S., Giannelli A., Brianti E., Dantas-Torres F., Bufalini M., Fraulo M., et al. 2014. Chronic polyarthritis associated to Cercopithifilaria bainae infection in a dog. Veterinary Parasitology, 205, 401–404. CrossrefWeb of ScienceGoogle Scholar

  • Giannelli A., Ramos R.A.N., Di Paola G., Mencke N., Dantas-Torres F., Baneth G., et al. 2013. Transstadial transmission of Hepatozoon canis from larvae to nymphs of Rhipicephalus sanguineus. Veterinary Parasitology, 196, 1–5. CrossrefWeb of SciencePubMedGoogle Scholar

  • Inokuma H., Okuda M., Ohno K., Shimoda K., Onishi T. 1992. Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Veterinary Parasitology, 106, 265–271Google Scholar

  • Latrofa M.S., Dantas-Torres F., Giannelli A., Otranto D. 2014. Molecular detection of tick-borne pathogens in Rhipicephalus sanguineus group ticks. Ticks and Tick-borne Diseases, 5, 943–946. CrossrefWeb of SciencePubMedGoogle Scholar

  • Lorusso V., Dantas-Torres F., Lia R.P., Tarallo V.D., Mencke N., Capelli G., et al. 2010. Seasonal dynamics of the brown dog tick, Rhipicephalus sanguineus, on a confined dog population in Italy. Medical and Veterinary Entomology, 24, 309–315. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Maia C., Ferreira A., Nunes M., Vieira M.L., Campino L., Cardoso L. 2014. Molecular detection of bacterial and parasitic pathogens in hard ticks from Portugal. Ticks and Tick Borne Diseases, 5, 409–414. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Miranda R.L., Castro J.R., Olegário M.M.M., Beletti M.E., Mundim A.V., O’Dwyer L.H., et al. 2011. Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog. Veterinary Parasitology, 177, 392–396. CrossrefWeb of SciencePubMedGoogle Scholar

  • O’Dwyer L.H. 2011. Brazilian canine hepatozoonosis. Brazilian Journal of Veterinary Parasitology, 20, 181–193.Google Scholar

  • O’Dwyer L.H., Massard C.L., Pereira de Souza J.C. 2001. Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil. Veterinary Parasitology, 94, 143–150.Google Scholar

  • Otranto D., Brianti E., Abramo F., Gaglio G., Napoli E., Latrofa M.S., et al. 2012. Cutaneous distribution and localization of Cercopithifilaria sp. microfilariae in dogs. Veterinary Parasitology, 190, 143–150. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Otranto D., Brianti E., Dantas-Torres F., Miró G., Latrofa M.S., Mutafchiev Y., et al. 2013a. Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region. Parasitology, 140, 99–108. CrossrefWeb of ScienceGoogle Scholar

  • Otranto D., Brianti E., Dantas-Torres F., Weigl S., Latrofa M.S., Gaglio G., et al. 2011. Morphological and molecular data on the dermal microfilariae of a species of Cercopithifilaria from a dog in Sicily. Veterinary Parasitology, 182, 221–229. CrossrefWeb of SciencePubMedGoogle Scholar

  • Otranto D., Varcasia A., Solinas C., Scala A., Brianti E., Dantas-Torres F., et al. 2013b. Redescription of Cercopithifilaria bainae Almeida & Vicente, 1984 (Spirurida, Onchocercidae) from a dog in Sardinia, Italy. Parasites and Vectors, 6, 132. CrossrefGoogle Scholar

  • Patton T.G., Dietrich G., Brandt K., Dolan M.C., Piesman J., Gilmore Jr R.D. 2012. Saliva, salivary gland, and hemolymph collection from Ixodes Scapularis ticks. Journal of Visionary Experimental, 1, 6. CrossrefGoogle Scholar

  • Ramos R.A.N., Giannelli A., Carbone D., Baneth G., Dantas-Torres F., Otranto D. 2014a. Occurrence of Hepatozoon canis and Cercopithifilaria bainae in an off-host population of Rhipicephalus sanguineus sensu lato ticks. Ticks and Tick Borne Diseases, 5, 311–314. CrossrefWeb of ScienceGoogle Scholar

  • Ramos R.A.N., Giannelli A., Lia R.P., Brianti E., Tarallo V.D., Breitshwerdt E.B., et al. 2014b. Incidence of Cercopithifilaria bainae in dogs and probability of co-infection with other tickborne pathogens. PLoS One, 9, 9–11. CrossrefGoogle Scholar

  • Ramos R.A.N., Rêgo A.G.O., Firmino E.D.F., Ramos C.A.N., Carvalho G.A., Dantas-Torres F., et al. 2016. Filarioids infecting dogs in northeastern Brazil. Veterinary Parasitology, 226, 26–29. CrossrefWeb of ScienceGoogle Scholar

  • Sanger F., Nicklen S., Coulson A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of National Academy of Sciences, 74, 5463–5467CrossrefGoogle Scholar

  • Santos M.A.B., Souza I.B., Macedo L.O., Ramos C.A.N., Rego A.G.O., Alves L.C., Ramos R.A.N., Carvalho G.A. 2017. Cercopithifilaria bainae in Rhipicephalus sanguineus sensu lato ticks from dogs in Brazil. Ticks and Tick Borne Diseases, 8, 623–625. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Silva V.D.P.R. 2004. On climate variability in Northeast of Brazil. Journal of Arid Environmental, 58, 575–596CrossrefGoogle Scholar

  • Sumrandee C., Baimai V., Trinachartvanit W., Ahantarig A. 2015. Hepatozoon and Theileria species detected in ticks collected from mammals and snakes in Thailand. Ticks and Tick Borne Diseases, 6, 309–315. CrossrefWeb of SciencePubMedGoogle Scholar

  • Walker J.B., Keirans J.E., Horak I.G. 2000. The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World. Cambridge University Press, CambridgeGoogle Scholar

About the article

Received: 2018-03-23

Revised: 2018-05-02

Accepted: 2018-05-04

Published Online: 2018-07-04

Published in Print: 2018-09-25


Citation Information: Acta Parasitologica, Volume 63, Issue 3, Pages 605–608, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0069.

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