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Acta Parasitologica

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Volume 63, Issue 3

Issues

Ascaridia galli isolates with ITS1-5.8rRNA-ITS2 fragment homologous to Ascaridia columbae

Joanna Urbanowicz
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Andrzej Gaweł
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kamila Bobrek
  • Corresponding author
  • Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland
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  • Other articles by this author:
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Published Online: 2018-07-04 | DOI: https://doi.org/10.1515/ap-2018-0073

Abstract

Ascaridia (A.) galli is one of the most commonly occurring nematodes in poultry worldwide, often in hens and broiler chickens. The infection with Ascaridia galli in free-range chickens was even 70%. There is not much information about A. galli genetic features. The present study was conducted to assess the genetic diversity of A. galli isolated from hens in Poland by analyzing the nucleotide sequence of the region ITS1-5.8rRNA-ITS2 and to define its homology within the family Ascaridiidae. Adult A. galli were collected from the intestines of naturally infected hens from two flocks of free-run laying hens from the Wielkopolska region in Poland. From all parasites an identical ITS1-5.8rRNA-ITS2 sequence was obtained, which was homologous in 99% with A. columbae (JQ995321.1) sequence. The high homology sequences of A. galli (KX683286) from Poland and A. columbae (JQ995321.1) isolate from the USA, support the observations of other authors suggesting that A. galli and A. columbae might be closely related. It is the first whole ITS1-5.8rRNA-ITS2 of A. galli in the GenBank database, so there is not enough data for detailed phylogenetic analysis of A. galli. Detailed genetic analysis is necessary to get better insight into the birds’ Ascaridia species.

Keywords: Ascaridia galli; ITS1-5; 8rRNA-ITS2; free-run hen

References

  • Adamson M. 1987. Phylogenetic analysis of the higher classification of the Nematoda. Canadian Journal of Zoology, 65, 1478–1482CrossrefGoogle Scholar

  • Anderson T.J. 2001. The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study. Trends in Parasitology, 17,183–188PubMedCrossrefGoogle Scholar

  • Blaxter ML., De Ley P., Garey J. R., Liu L.X., Scheldeman P., Vierstraete A., et al. 1998. A molecular evolutionary framework for the phylum Nematoda. Nature, 392, 71–75. CrossrefPubMedGoogle Scholar

  • Bazh E.K. 2013. Molecular characterization of Ascaridia galli infecting native chickens in Egypt. Parasitology Research, 112, 3223–3227. .CrossrefPubMedWeb of ScienceGoogle Scholar

  • Chadfield M.S., Permin A., Nansen P., Bisgaard M. 2001. Investigation of the parasitic nematode Ascaridia galli as a potential vector for Salmonella dissemination in broiler poultry. Parasitology Research, 87, 317–325CrossrefGoogle Scholar

  • Chilton N.B., Gasser R.B., Beveridge I. 1997. Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data. International Journal of Parasitology, 27, 1481–1494CrossrefGoogle Scholar

  • Długosz E., Wiśniewski M. 2006. Molecular diagnostic of parasites using rRNA gene sequence. Wiadomości Parazytologiczne, 52, 263–269PubMedGoogle Scholar

  • Everett E., Hwang J.C., Hwang W. 1914. The Life Cycle and Morphology of Ascaridia columbae (Gmelin, 1790) Travassos, (Nematoda:Ascarididae) in the Domestic Pigeon (Columba livia domestica). Journal of Parasitology, 50, 131–137Google Scholar

  • Höglund J., Morrison D.A., Engström A., Nejsum P., Jansson D.S. 2012. Population genetic structure of Ascaridia galli re-emerging in non-caged laying hens. Parasites and Vectors, 20, 5–9. CrossrefGoogle Scholar

  • Inglis W.G. 1983. An outline classification on the Phylum Nematoda. Australian Journal of Zoology, 31, 243–255CrossrefGoogle Scholar

  • Lin Q., Li H.M., Gao M., Wang X.Y., Ren W.X, Cong M.M., et al. 2012. Characterization of Baylisascaris schroederi from Qinling subspecies of giant panda in China by the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA. Parasitology Research, 110, 1297–1303. CrossrefPubMedWeb of ScienceGoogle Scholar

  • Liu G.H., Shao R., Li J.Y., Zhou D.H., Li H., Zhu X.Q. 2013. The complete mitochondrial genomes of three parasitic nematodes of birds: a unique gene order and insights into nematode phylogeny. BMC Genomics, 21, 414. CrossrefWeb of ScienceGoogle Scholar

  • Luna-Olivares L.A., Ferdushy T., Kyvsgaard N.C., Nejsum P., Thamsborg S.M., Roepstorff A. et al. 2012. Localization of Ascaridia galli larvae in the jejunum of chickens three days post infection. Veterinary Parasitology, 185, 186–193. CrossrefGoogle Scholar

  • Magwisha H., Kassuku A., Nyvsgaard N., Permin A. 2002. A comparison of the prevalance and burdens of helminth infections in growers and adult free-range chickens. Tropical Animal Health Production, 34, 205–214CrossrefGoogle Scholar

  • Martin-Pacho J.R., Montoya M.N., Arangna T., Toro C., Morchon C., Marcos-Atxutegi C. et al. 2005. A coprological and serological survery for the prevalence of Ascaridia spp. in Laying Hens. Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 52, 238–242. CrossrefGoogle Scholar

  • Permin, A., Bisgaard M., Frandsen F., Pearman M., Kold J., Nansen P. 1999. Prevalence of gastrointestinal helminths in different poultry production systems. British Poultry Science, 40, 439–443. CrossrefPubMedGoogle Scholar

  • Permin A., Christensen J.P, Bisgaard M. 2006. Consequences of concurrent Ascaridia galli and Escherichia coli infections in chickens. Acta Veterinaria Scandinavica, 47, 43–54PubMedCrossrefGoogle Scholar

  • Ramadan H.H., Abouznada N.Y. 1992. Morphology and life history of Ascaridia galli in the Domestic Fowl that are raised in Jeddah. Journal of King Saud UniversityScience, 4, 87–99Google Scholar

  • Schnare M.N., Collings J.C., Spencer D.F, Gray. M.W. 2000. The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor. Nucleic Acids Research, 28, 3452–3461CrossrefPubMedGoogle Scholar

  • Szelągiewicz, M., Sokół R. 1991. Parasites of poultry in a small on suburb raisings. Medycyna Weterynaryjna, 47, 208–209Google Scholar

  • Veldman G.M., Klootwijk J., Van Heerikhuizen H., Planta R.J. 1981. The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript. Nucleic Acids Research, 9, 4847–4862CrossrefPubMedGoogle Scholar

  • Zhao W.T., Guo Y.N., Zhang L.P., Li L. 2016. Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae). Acta Parasitolologica, 61, 66–73. CrossrefGoogle Scholar

About the article

Received: 2017-12-04

Revised: 2018-03-26

Accepted: 2018-04-04

Published Online: 2018-07-04

Published in Print: 2018-09-25


Citation Information: Acta Parasitologica, Volume 63, Issue 3, Pages 640–644, ISSN (Online) 1896-1851, ISSN (Print) 1230-2821, DOI: https://doi.org/10.1515/ap-2018-0073.

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